Non-transferrin donors of iron for heme synthesis in immature erythroid cells

Fuchs, Ota; Borová, J; Hradilek, A; Neuwirt, J

doi:10.1016/0167-4889(88)90071-7

Cited by 18 publications

(6 citation statements)

References 31 publications

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“…Further, when reticulocytes were preincubated with NH 4 Cl for 30 min prior to measuring iron incorporation from ferric citrate, iron transport was not blocked. In contrast, incorporation of iron derived from transferrin iron was inhibited by NH 4 Cl (Fuchs et al, 1988). These observations suggested that the uptake of iron from iron salts such as ferric citrate was independent of the transferrin-transferrinreceptor pathway.…”

Section: The Iron Citrate Pathway Was Independent Of Transferrincontrasting

confidence: 38%

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The alternate iron transport pathway: mobilferrin and integrin in reticulocytes

et al. 1997

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Iron transport in reticulocytes is known to occur via the well‐described transferrin‐receptor–endosome pathway. An alternative pathway for iron transport independent of transferrin has been postulated in reticulocytes and other cells. Transport of iron into reticulocytes from ferric citrate solutions was shown to be saturable and independent of transferrin. During transport of iron from ferric citrate, both cell surface integrins, and a soluble protein, mobilferrin, were labelled. This demonstrated that the reticulocyte transferrin independent pathway for iron transport involved integrins and mobilferrin similar to intestinal absorptive cells. This pathway would be expected to transport iron into cells under conditions of iron overload and was capable of providing iron for haemoglobin synthesis. Mobilferrin was also radiolabelled when radioiron labelled transferrin was incubated with reticulocytes and this occurred with a different time course than was observed following reticulocyte exposure to radiolabelled ferric citrate. This suggested that mobilferrin may serve as an intermediary in both pathways.

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Section: The Iron Citrate Pathway Was Independent Of Transferrincontrasting

confidence: 38%

“…The transport of transferrin into rat reticulocytes was inhibited by blocking antibody to the transferrin receptor (Fuchs et al, 1988). When 10 mg of monoclonal antibody was added to reticulocytes about two-thirds of the transferrin uptake was blocked (Fig 2).…”

Section: The Iron Citrate Pathway Was Independent Of Transferrinmentioning

confidence: 96%

The alternate iron transport pathway: mobilferrin and integrin in reticulocytes

et al. 1997

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“…The ability to utilize iron from simple iron salts has also been described for other cell types, e.g. reticulocytes [54][55][56], and L1210 cells [57].…”

Section: Thorstensen Unpublished Work)mentioning

confidence: 99%

The role of transferrin in the mechanism of cellular iron uptake

Thorstensen

Romslo

1990

Biochemical Journal

185

106

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INTRODUCTIONThe role of transferrin and its cell surface receptor in cellular iron metabolism has received considerable interest during the last decade. Hence, over this period several reviews covering various aspects of the topic have been published.In this review we attempt to summarize the new knowledge and controversies encountered during the last 4-5 years. The focus is on iron metabolism by the hepatocyte. We also view the concept of transferrin-cell interactions from angles which previously may not have been given particular attention, and we include some aspects of iron metabolism not normally covered by reviews of this type. Central parts of what may be regarded as well-established knowledge either have been entirely omitted, or are only briefly described or mentioned to the extent considered relevant to the particular topic discussed. Important aspects of iron metabolism such as iron adsorption, haemochromatosis, ferritin iron and iron in infection and neoplasia are not covered in the present paper. Instead, the reader is referred to one or more of the recently published reviews [1-5] and references therein.

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“…Based on studies where cells were grown in serumfree, hence Tf-free, media and in cases of iron overload disorders, it has become evident that some cells are able to obtain Fe independently of Tf and the RME pathway. For example, a number of cell types have been shown to express transport systems capable of accumulating Fe from low molecular weight Fe chelates (Brissot et al, 1985;Basset et al, 1986;Wright et al, 1986;Egyed, 1988;Fuchs et al, 1988;Morgan, 1988;Sturrock et al, 1990;Kaplan et al, 1991;Olakanmi et al, 1994). Mutant Chinese hamster ovary (CHO) cells that were unable to release Fe from Tf (Klausner et al, 1984) as well as CHO cells without TR (McGraw et al, 1987;Chan et al, 1992) were both able to take up Fe from Fe salts and grow as well as normal CHO cells.…”

Section: Introductionmentioning

confidence: 99%

A novel iron uptake mechanism mediated by GPI-anchored human p97.

et al. 1995

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The established process for iron uptake into mammalian cells involves transferrin and its receptor. Here, the role of the glycosyl‐phosphatidylinositol (GPI)‐linked transferrin homologue, melanotransferrin or p97, was studied using CHO cell lines defective in the transferrin receptor (TR) and transfected with human TR and/or human p97. The presence of p97 doubled the iron uptake, which could be explained by the binding of one atom of iron to one molecule of p97. The internalization of iron was shown to be temperature sensitive and saturated at a media iron concentration of 2.5 micrograms/ml with a Vmax of 0.1 pmol Fe/10(6) cell/min and a Km of 2.58 microM for p97. Treatment of the cells with either phosphatidylinositol‐phospholipase C or monoclonal antibodies against p97 resulted in over a 50% reduction and a 47% increase in the iron uptake respectively. These data identify p97 as a unique cell surface GPI‐anchored, iron binding protein involved in the transferrin‐independent uptake of iron in mammals.

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Non-transferrin donors of iron for heme synthesis in immature erythroid cells

Cited by 18 publications

References 31 publications

The alternate iron transport pathway: mobilferrin and integrin in reticulocytes

The alternate iron transport pathway: mobilferrin and integrin in reticulocytes

The role of transferrin in the mechanism of cellular iron uptake

A novel iron uptake mechanism mediated by GPI-anchored human p97.

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