Non-toxic plant metabolites regulate<i>Staphylococcus</i>viability and biofilm formation: a natural therapeutic strategy useful in the treatment and prevention of skin infections

Morán, A.; Gutiérrez, Sergio; Martı́nez-Blanco, Honorina; Ferrero, Miguel Ángel Martín; Monteagudo-Mera, Andrea; Rodrı́guez-Aparicio, Leandro B.

doi:10.1080/08927014.2014.976207

Cited by 53 publications

(41 citation statements)

References 48 publications

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“…Biofilm accumulation by S. epidermidis was much higher than for S. aureus , in all cases. This agrees with previous observations that S. epidermidis is a more prolific biofilm former than S. aureus (Morán et al ). After 48 h, S. epidermidis developed thick, highly structured biofilm matrices on all surface types, and covered a significant portion of the surface area, especially on the nanosmooth, 50 and 100 nm diameter pore surfaces.…”

Section: Resultssupporting

confidence: 93%

Nanoporous anodic alumina reduces Staphylococcus biofilm formation

Feng

Cheng

Worobo

et al. 2019

Lett Appl Microbiol

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Staphylococcus epidermidis and Staphylococcus aureus, two bacterial strains commonly associated with biofilm‐related medical infections and food poisoning, can rapidly colonize biotic and abiotic surfaces. The present study investigates the ability of anodic alumina surfaces with nanoporous surface topography to minimize the attachment and biofilm formation mediated by these pathogenic bacterial strains. Early attachment and subsequent biofilm development were retarded on surfaces with nanopores of 15–25 nm in diameter compared to surfaces with 50–100 nm pore diameter and nanosmooth surfaces. After 30 min of incubation in nutritive media, the biomass accumulation per unit surface area was 2·93 ± 1·72 µm3 µm‐2 for the 15 nm, 3·49 ± 1·97 µm3 µm‐2 for the 25 nm, as compared to 14·04 ± 6·39 µm3 µm‐2 for the nanosmooth, 11·88 ± 9·72 µm3 µm‐2 for the 50 nm and 12·09 ± 11·84 µm3 µm‐2 for the 100 nm surfaces respectively. These findings suggest that anodic alumina with small size nanoscale pores could reduce the incidence of staphylococcal biofilms and infections, and shows promise as a material for a variety of medical applications and food contact surfaces. Significance and Impact of the Study This paper reports on a simple, robust and scientifically sound method to reduce attachment and biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis to abiotic surfaces using a carefully designed nanoscale topography. This approach can help to reduce the incidence of staphylococcal biofilms and infections without imposing selective stresses on bacteria, thus preventing the creation of resistant strains.

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Section: Resultssupporting

confidence: 93%

Nanoporous anodic alumina reduces Staphylococcus biofilm formation

Feng

Cheng

Worobo

et al. 2019

Lett Appl Microbiol

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“…44 reported that MRSA strains were not inhibited by soy isoflavones, including genistein and daidzein (up to 100 μg/mL), and Morán et al . 45 found no MIC for genistein up to 2 mg/mL against S . aureus CECT 59.…”

Section: Discussionmentioning

confidence: 94%

Looking to nature for a new concept in antimicrobial treatments: isoflavonoids from Cytisus striatus as antibiotic adjuvants against MRSA

Abreu

Coqueiro

Sultan³

et al. 2017

Sci Rep

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The spread of multidrug-resistant Staphylococcus aureus strains, including methicillin-resistant S. aureus (MRSA), has shortened the useful life of anti-staphylococcal drugs enormously. Two approaches can be followed to address this problem: screening various sources for new leads for antibiotics or finding ways to disable the resistance mechanisms to existing antibiotics. Plants are resistant to most microorganisms, but despite extensive efforts to identify metabolites that are responsible for this resistance, no substantial progress has been made. Plants possibly use multiple strategies to deal with microorganisms that evolved over time. For this reason, we searched for plants that could potentiate the effects of known antibiotics. From 29 plant species tested, Cytisus striatus clearly showed such an activity and an NMR-based metabolomics study allowed the identification of compounds from the plant extracts that could act as antibiotic adjuvants. Isoflavonoids were found to potentiate the effect of ciprofloxacin and erythromycin against MRSA strains. For the structure-activity relationship (SAR), 22 isoflavonoids were assessed as antibiotic adjuvants. This study reveals a clear synergy between isoflavonoids and the tested antibiotics, showing their great potential for applications in the clinical therapy of infections with antibiotic-resistant microorganisms such as MRSA.

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“…SIC of eugenol (≤50 μg/mL) can significantly inhibit the growth of Staphylococcus aureus and Staphylococcus epidermidis (Morán et al., ). When S. carnosus is treated with eugenol (6 to 8.7 μL/mL) for 0, 3, 4, 6, and 8 hr, the highest antibacterial activity is detected after the addition of eugenol for 3 to 4 hr (Manrique, Gibis, Schmidt, & Weiss, ).…”

Section: The Antibacterial Actions and Mechanisms Of Clove Oil And Eumentioning

confidence: 99%

Progress on the Antimicrobial Activity Research of Clove Oil and Eugenol in the Food Antisepsis Field

Zhou

Wei

2018

Journal of Food Science

108

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As potential and valuable antiseptics in the food industry, clove oil and its main effective composition eugenol show beneficial advantages on antibacterial and antifungal activity, aromaticity, and safety. Researches find that both clove oil and eugenol express significantly inhibitory effects on numerous kinds of food source microorganisms, and the mechanisms are associated with reducing the migratory and adhesion and inhibiting the synthesis of biofilm and various virulence factors of these microorganisms. Clove oil and eugenol are generally regarded as safe in vivo experiments. However, they may express certain cytotoxicity on fibroblasts and other cells in vitro. Studies on the quality and additive standard of clove oil and eugenol should be strengthened to promote the antiseptic effects of them in the food antiseptic field.

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Non-toxic plant metabolites regulateStaphylococcusviability and biofilm formation: a natural therapeutic strategy useful in the treatment and prevention of skin infections

Cited by 53 publications

References 48 publications

Nanoporous anodic alumina reduces Staphylococcus biofilm formation

Nanoporous anodic alumina reduces Staphylococcus biofilm formation

Looking to nature for a new concept in antimicrobial treatments: isoflavonoids from Cytisus striatus as antibiotic adjuvants against MRSA

Progress on the Antimicrobial Activity Research of Clove Oil and Eugenol in the Food Antisepsis Field

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