Non-toxic phototriggered gene transfection by PAMAM-porphyrin conjugates

Shieh, Ming‐Jium; Peng, Cheng‐Liang; Lou, Pei‐Jen; Chiu, C.-S.; Tsai, Tsiao-Yu; Hsu, Chih-Yu; Yeh, Chen‐Yu; Lai, Ping‐Shan

doi:10.1016/j.jconrel.2008.03.024

Cited by 94 publications

(52 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1-3 Specifically, phototriggered theranostics, combining phototherapies (for example, photodynamic, 4,5 photothermal, 6,7 and phototriggered chemo 8,9 / gene therapy 10 ) with real-time photodiagnostics (for example, bioluminescence, 11 fluorescence, 12,13 and photoacoustic imaging 14,15 ) have been actively pursued due to the advantages of spatiotemporal selectivity, non-ionizing radiation and specificity for disease destruction. Among them, near-infrared (NIR) light-triggered photodynamic theranostics, in which nanoparticles are used as photodynamic therapy (PDT) agents, have recently been receiving much attention due to deep penetration and simultaneous generation of reactive oxygen species (ROS) upon the NIR light irradiation, leading to ablation of cancer cells as a result.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional upconversion–nanoparticles–trismethylpyridylporphyrin–fullerene nanocomposite: a near-infrared light-triggered theranostic platform for imaging-guided photodynamic therapy

Guan

Dong

et al. 2015

NPG Asia Mater

View full text Add to dashboard Cite

Photodynamic therapy (PDT) is an excellent therapeutic modality for various malignant and nonmalignant cancers. This approach utilizes reactive oxygen species generated through the reaction between photosensitizer and oxygen in tissues upon light irradiation to achieve effective treatment. However, limited penetration depth and oxygen-deficient microenvironment hinder the efficiency of PDT. In this work, we design a multifunctional near-infrared (NIR)-triggered theranostic agent based on upconversion-nanoparticles-Polyoxyethylene bis (amine)-trismethylpyridylporphyrin-fullerene nanocomposite (UCNP-PEG-FA/ PC 70 ) for imaging (fluorescence/upconversion luminescence/magnetic resonance imaging)-guided photodynamic therapy. In this multimodal nanocompsite, UCNPs are employed as light transducers to convert NIR light into ultraviolet-visible light to activate PC 70 to generate singlet oxygen ( 1 O 2 ) even under low-oxygen conditions. Meanwhile, the upconversion emission, magnetic resonance imaging and fluorescence signal coming from UCNPs and PC 70 nanocomposite enable UCNP-PEG-FA/PC 70 to act as a multimodal imaging diagnostic agent, which facilitates the imaging-guided PDT. Furthermore, folate-mediated active targeting would enhance the accumulation of multifunctional hybrid in tumor. In vitro as well as in vivo results suggest that this smart nanocomposite is promising as an NIR light-triggered and -targeted theranostic platform for imaging-guided PDT of cancer, which may provide a solution to the bottleneck problems of PDT, namely, limited penetration depth and oxygen-deficient microenvironment.

show abstract

Section: Introductionmentioning

confidence: 99%

Multifunctional upconversion–nanoparticles–trismethylpyridylporphyrin–fullerene nanocomposite: a near-infrared light-triggered theranostic platform for imaging-guided photodynamic therapy

Guan

Dong

et al. 2015

NPG Asia Mater

View full text Add to dashboard Cite

show abstract

“…They have been used in light-activated cancer treatment (photodynamic therapy) [1], in sensor design due to their fluorescent and electrochemical properties [2] and in gene therapy [3], they are the functional elements of light harvesting systems [4] and they could be used as artificial endonucleases [5].…”

Section: Introductionmentioning

confidence: 99%

Syntheses and DNA binding of new cationic porphyrin–tetrapeptide conjugates

Mező

Herényi

Habdas

et al. 2011

Biophysical Chemistry

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT AbstractRecently cationic porphyrin-peptide conjugates were synthesized to enhance the cellular uptake of porphyrins or deliver the peptide moiety to the close vicinity of nucleic acids. DNA binding of such compounds was not systematically studied yet.We synthesized two new porphyrin-tetrapeptide conjugates which can be considered as a typical monomer unit corresponding to the branches of porphyrin-polymeric branched chain polypeptide conjugates. Tetra-peptides were linked to the tri-cationic meso-tri A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT Graphical AbstractResearch Highlights We synthesized two new porphyrin-tetrapeptide conjugates. DNA binding of porphyrin conjugates was studied with spectroscopic methods. Peptide conjugates of cationic porphyrins bind to DNA by two distinct binding modes. Binding modes can be identified as intercalation and external binding.

show abstract

“…It was later employed as a tool to improve the cellular delivery of a large variety of bioactive macromolecules and nucleic acids including pDNA, siRNA and oligonucleotides (Selbo, Weyergang et al 2010). Examples of photosensitizers that are used in non-viral nucleic acid delivery including disulfonated meso-tetraphenylporphine (TPPS2a) (Prasmickaite, Høgset et al 2000;Kloeckner, Prasmickaite et al 2004;Maurice-Duelli, Ndoye et al 2004;Ndoye, Merlin et al 2004;Ndoye, Dolivet et al 2006;Oliveira, Fretz et al 2007;Boe, Longva et al 2008;Raemdonck, Naeye et al 2009;Bøe, Saebøe-Larssen et al 2010), disulfonated aluminium phthalocyanine (AlPcS2a) (Berg, Prasmickaite et al 2003;Hellum, Høgset et al 2003;Ndoye, Dolivet et al 2006;Yip, Weyergang et al 2007), Zincphthalocyanine (Zn-Pc) dendrimer (Nishiyama, Iriyama et al 2005;Arnida, Nishiyama et al 2006) and 5,10,15-tri(4-acetamidophenyl)-20-mono(4-carboxyl-phenyl)porphyrin (TAMCPP) conjugated to G4 PAMAM dendrimer (Shieh, Peng et al 2008). To employ PCI in clinical applications, the penetration of light into the deep tissue is an important issue (Oliveira, Fretz et al 2007).…”

Section: Photochemical Internalization (Pci)mentioning

confidence: 99%