Non-toxic and toxic subclones obtained from a toxic clonal culture of Alexandrium tamarense (Dinophyceae): Toxicity and molecular biological feature

Cho, Yuko; Hiramatsu, Kazuhisa; Ogawa, Motoo; Omura, Takuo; Ishimaru, Takashi; Oshima, Yasukatsu

doi:10.1016/j.hal.2008.02.008

Cited by 39 publications

(33 citation statements)

References 42 publications

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“…minutum (McCauley et al, 2009), using microsatellite markers. This, in combination with the results of strains with differing toxin production following intraspecific mating experiments (Cho et al, 2008), suggests that significant population level differences in toxin production may also exist within A. tamarense (Group V). Further studies using microsatellite markers on multiple clonal cultures of this strain may help to determine whether toxin production is restricted to one particular population of this species, which may allow for the design of predictive genetic tools for the identification of this population.…”

Section: Discussionmentioning

confidence: 74%

“…The non-toxic characteristics of one strain of A. tamarense, an axenic non-toxic subclone of a toxic strain, were confirmed at the attomole per cell level. Three out of nine toxic subclones of this same strain became non-toxic over a relatively short period of time (4-6 years), while the other toxic subclones retained their toxicity and the non-toxic subclones remained non-toxic (Cho et al, 2008).…”

Section: Discussionmentioning

confidence: 94%

“…Mating experiments have found that subclones of a toxic clonal strain of A. tamarense (Group IV) can be non-toxic (Cho et al, 2008). The non-toxic characteristics of one strain of A. tamarense, an axenic non-toxic subclone of a toxic strain, were confirmed at the attomole per cell level.…”

Section: Discussionmentioning

confidence: 95%

See 2 more Smart Citations

A reinvestigation of saxitoxin production and sxtA in the ‘non-toxic’ Alexandrium tamarense Group V clade

et al. 2012

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

A reinvestigation of saxitoxin production and sxtA in the ‘non-toxic’ Alexandrium tamarense Group V clade

et al. 2012

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“…Yé pré mian et al, 2007) and marine environments for the STXs producers Alexandrium and Gymnodinium (e.g. Cho et al, 2008;Touzet et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Evidence for saxitoxins production by the cyanobacterium Aphanizomenon gracile in a French recreational water body

et al. 2010

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“…While it is impossible to rule out a laboratory labelling error, in this case both the toxic and non-toxic counterpart have been confirmed to possess the same morphological features, identical ribosomal rDNA type (Martins et al 2004) and similar bacterial communities at a species level, but with several types unique to each culture (Palacios et al 2006). The authors proposed that spontaneous mutation was an alternative explanation for the observed loss of toxicity, and this suggestion is partially borne out by the work and observations of Cho et al (2008).…”

Section: Bacterial Community Influence On Dinoflagellate Cell Toxicitymentioning

confidence: 92%

Bacterial diversity of Gymnodinium catenatum and its relationship to dinoflagellate toxicity

Green

Hart

Blackburn

et al. 2010

Aquat. Microb. Ecol.

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Gymnodinium catenatum Graham (Dinophyceae) is one of several marine dinoflagellates responsible for outbreaks of paralytic shellfish poisoning (PSP), a problem that is considered to be increasing globally. Bacteria associated with these dinoflagellates have been implicated as potentially involved with the production of PSP toxins, and this study sought to identify whether there was a link between the toxicity of G. catenatum laboratory cultures and the diversity of the associated bacterial community. Bacterial 16S rRNA gene clone libraries were constructed and sequenced to identify the bacterial diversity of 7 G. catenatum cultures of 2 contrasting toxicity levels. Phylogenetic membership and community structure were examined, including the use of UniFrac, F ST and LIB-COMPARE. No statistically significant differences that distinguished between toxic and low-toxicity G. catenatum cultures were identified in the bacterial community membership or structure. Furthermore, no coherent phylogenetic group of bacteria was observed to co-associate with culture toxicity. However, observed variation in bacterial diversity and community structure was based on the geographic origin of the G. catenatum cultures. Overall, while it was not possible to identify an apparent link between bacterial diversity and the toxicity of G. catenatum cultures, we suggest, on the balance of this study and others, that bacterial influence on PSP toxin production may be indirect and mediated by the effects of the bacterial community on algal physiology. KEY WORDS: Gymnodinium catenatum · Paralytic shellfish toxins · 16S rDNA bacterial diversity · Algae-associated bacteria Resale or republication not permitted without written consent of the publisher

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Non-toxic and toxic subclones obtained from a toxic clonal culture of Alexandrium tamarense (Dinophyceae): Toxicity and molecular biological feature

Cited by 39 publications

References 42 publications

A reinvestigation of saxitoxin production and sxtA in the ‘non-toxic’ Alexandrium tamarense Group V clade

A reinvestigation of saxitoxin production and sxtA in the ‘non-toxic’ Alexandrium tamarense Group V clade

Evidence for saxitoxins production by the cyanobacterium Aphanizomenon gracile in a French recreational water body

Bacterial diversity of Gymnodinium catenatum and its relationship to dinoflagellate toxicity

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