Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of U251SP glioblastoma

Kurake, Naoyuki; Ishikawa, Kenji; Tanaka, Hiromasa; Hashizume, Hiroshi; Nakamura, Kae; Kajiyama, Hiroaki; Toyokuni, Shinya; Kikkawa, Fumitaka; Mizuno, Masaaki; Hori, Masaru

doi:10.1016/j.abb.2018.12.001

Cited by 33 publications

(20 citation statements)

References 56 publications

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“…Our previous studies have shown that the application of nonthermal argon gas plasma under appropriate conditions can effectively optimize the germination rate of soybeans (7), the development of chicken embryos (8), the growth of chickens (9) and the quality of sperm (10) by regulating redox homeostasis, energy metabolism and DNA methylation. Recently, due to its stability, low cost, and easy application to targeted tissues with excellent effect, a plasma-activated solution [mainly including plasma-activated water and plasma-activated medium without cells (PAM)] has been applied to various cancer cell lines, including those of glioblastoma (11), colon cancer (12), and breast cancer and lung cancer (13).…”

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confidence: 99%

“…NTP treatment of CT26 colorectal cancer cells induced T-cell responses through targeting guanylyl cyclase C (17). In glioblastoma cells, PAM with high concentrations of H 2 O 2 and NO 2 induced down-regulation of the AKT and extracellular signalregulated kinase (ERK) signaling pathways, inhibition of the glycolysis pathway and enhancement of the pentose phosphate pathway (11,18). Prostate cancer cells, with a background of high energy metabolism and higher oxidative conditions, cause them to be more vulnerable to plasmainduced injury (19).…”

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confidence: 99%

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Non-thermal Plasma-activated Medium Induces Apoptosis of Aspc1 Cells Through the ROS-dependent Autophagy Pathway

Xing

Sun

Liu

et al. 2019

In Vivo

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Background/Aim: Numerous studies on various cancer cell lines have reported that direct exposure to nonthermal plasma treatment using plasma-activated medium (PAM) can be applied as a novel technology for cancer therapy. In this study, we investigated the inhibitory effects of PAM on Aspc1 pancreatic cancer cells and the mechanisms responsible for the cell death observed. Materials and Methods: A colony-formation, sphereformation, wound-healing and transwell assays, immunocytochemistry and western blot analysis were used monitor effects of PAM. Results: PAM induced a greater cytotoxic effect in pancreatic cancer cells compared to that induced in NIH3T3 cells and 293T cells, and significantly inhibited colony and sphere formation, and cell migration of Aspc1 cells. Furthermore, PAM treatment increased the accumulation of reactive oxygen species (ROS) and reduced the mitochondrial membrane potential in Aspc1 cells. In addition, PAM treatment down-regulated the AKT serine/threonine kinase 1/signal transducer and activator of transcription 3 signaling pathway and induced ROSdependent cellular autophagy. Conclusion: Our findings suggest that PAM can induce apoptosis of Aspc1 cells through ROS-dependent autophagy and may be a candidate for use in pancreatic cancer therapeutics.

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confidence: 99%

mentioning

confidence: 99%

Non-thermal Plasma-activated Medium Induces Apoptosis of Aspc1 Cells Through the ROS-dependent Autophagy Pathway

Xing

Sun

Liu

et al. 2019

In Vivo

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“…Further in vitro experiments that utilized gas plasma-oxidized liquids for cancer treatment made further suggestions for plasma effects playing an important role in the formation of cell death dependent on autophagy [50,52], the downregulation of survival proteins that are involved in the AKT pathway [87,88], targeting cell organelles like mitochondria and the endoplasmic reticulum [89][90][91], and redox-regulated changes in cell pathway activation and gene expression [92][93][94][95]. Moreover, alteration of the metastatic capacities of tumor cells [60,96] and their morphology, oxidation of the membrane [76,97,98], senescence (through intracellular zink) [36,45], and disturbances in the cells' metabolic profile [47] are examples of promising research. Out of several in vivo experiments, there are only three that were performed in syngeneic cancer animal models.…”

Section: Mechabisms Of Action and Selection Of Suitable Liquidsmentioning

confidence: 99%

Gas Plasma-Oxidized Liquids for Cancer Treatment: Preclinical Relevance, Immuno-Oncology, and Clinical Obstacles

Freund

Bekeschus

2021

IEEE Trans. Radiat. Plasma Med. Sci.

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Gas plasmas, often referred to as cold physical plasma, are currently being investigated for their potential to serve as anti-cancer agents. Along similar lines, gas plasma-oxidized liquids as a carrier for reactive oxygen species have found their way into pre-clinical research. This review focuses on in vivo studies that utilized such gas plasma-oxidized liquids for cancer therapies. These pre-clinical tumor models, treatment modalities, and types of liquids that were used are summarized and critically discussed. Among these studies, significant results were observed, indicating the potential of oxidative liquids to serve as an anticancer treatment. However, several steps have to be taken to enhance the quality and translational capacities of this approach in order to gain clinical acceptance for possible future cancer therapies. The most crucial steps include not only a careful selection of suitable liquids, with respect to their approval as medical products, but also the consideration of orthotopic and immunocompetent animal tumor models. This would increase the relevance of such studies and simultaneously allow studying the contribution of the most potent of all anti-cancer effectors, the immune system.

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“…Именно с нарушением последних двух процессов авторы [63] связали деплецию НАД + под действием продуктов ХП. Действительно, подавление гликолиза и снижение уровня внутриклеточного НАД + были зарегистрированы при действии ХП в различных клетках, что потенцировалось применением ингибиторов гликолиза [64,65].…”

Section: влияние хп на метаболизм клетокunclassified

Chemical mechanisms of non-thermal plasma action on cells

Оловянникова

Makarenko

Лычковская

et al. 2020

Fundamentalʹnaâ i kliničeskaâ medicina

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Non-thermal plasma (NTP) in the air around the cell layer or biological tissues is considered as a generator of reactive oxygen and nitrogen species, ions, and solvated/aquated electrons. This review covers current understanding on the effects of NTP in living systems, with the focus on the role of free radicals and other NTP-generated particles in the chemical modification of biomacromolecules and regulation of signal transduction. We summarise recent data on the impact of NTP-originated products on intracellular redox balance, mitochondrial biogenesis, cell membranes and organelles. In addition, we discuss the transport of NTP products across the biological membranes. Since the expression of numerous transporter systems differs at various stages of development, distinct cell lines, and in pathological conditions, experiments on NTP effects should be designed in various models for the assessment of cell- and tissue-specific response. Notably, NTP effects are observed throughout the whole tissue even when particles are generated at the surface. Special attention is paid to the NTP-treated solutions (phosphate buffered saline, Ringer’s solution, cell culture medium) as their composition and pH can be significantly altered. However, these data also suggest novel opportunities for the application of NTP and NTP-treated solutions in biomedicine. Studies on the mechanisms of NTP action on biological systems should contain analysis of events coupled to generation and accumulation of reactive oxygen and nitrogen species, neutral compounds, solvated electrons, and detection of new cellular targets of their action. This would allow developing of efficient and safe protocols for NTP applications in biology and medicine.

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Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of U251SP glioblastoma

Cited by 33 publications

References 56 publications

Non-thermal Plasma-activated Medium Induces Apoptosis of Aspc1 Cells Through the ROS-dependent Autophagy Pathway

Non-thermal Plasma-activated Medium Induces Apoptosis of Aspc1 Cells Through the ROS-dependent Autophagy Pathway

Gas Plasma-Oxidized Liquids for Cancer Treatment: Preclinical Relevance, Immuno-Oncology, and Clinical Obstacles

Chemical mechanisms of non-thermal plasma action on cells

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