Non-T, non-B acute lymphocytic leukemias: Cellular origin based on molecular analyses of immunoglobulin and T-cell .ALPHA.- and .BETA.-chain receptor gene rearrangements.

Correlation of morphologic and cytochemical diagnosis with flowcytometric analysis in acute leukemia

“…However, further evaluation of these cases with a secondary panel of specific markers like cyCD22 and CD79a for B-ALL and cyCD3 for T-ALL could not be done due to the non-availability of these markers. Okabe et al [20] have evaluated 15 such cases in their study and tried to classify these cases based on molecular studies that included immunoglobulin and T-cell receptor genes. Their study revealed that these cases showed gene rearrangements at J H genes and supported the view that most CALLA-positive cells are neoplastic B-cell precursors.…”

Detection of Clone-Specific Immunoglobulin Heavy Chain Genes in the Bone Marrow of B-cell-Lineage Lymphoma after Treatment

“…In this respect, it is important to determine the involvement of the bone marrow and peripheral blood in NHL. This has often been hampered because of the poor sensitivity of detection by morphological or Southern blot analysis (Okabe et al 1987), which represents the gold standard methods for clonality assessment. Polymerase chain reaction (PCR) methods are clearly more sensitive than other methods, allowing immunophenotyping and cytogenetic analysis (Bagg and Kallakury 1999).…”

Non-B, Non-T Acute Lymphoblastic Leukemia in a Cat

“…Non-B, non-T cell lymphoproliferative diseases are characterized by the absence of B-cell or T-cell-specific antigens and instead express common lymphocyte antigens. Compared to studies of non-T leukemia in human ALL (6,9), there is limited research available on non-B, non-T cell-origin lymphocytic leukemia in veterinary medicine. To date, two out of five cases of ALL in dogs (14) and only one cat have been reported for non-B, non-T lymphoma/leukemia (5).…”