Non-systemic metamorphosis in male millipede appendages: long delayed, reversible effect of an early localized positional marker?

Drago, Leandro; Fusco, Giuseppe; Minelli, Alessandro

doi:10.1186/1742-9994-5-5

Cited by 15 publications

(17 citation statements)

References 10 publications

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“…Standard genotyping methods often cannot be easily applied to FFPE specimens. Particularly, formalin fixation may cause extensive DNA damage, including cross-linking and fragmentation [ 12 , [14] , [15] , [16] , [17] , [18] ]. Several techniques for the molecular diagnosis of HPV, ranging from conventional PCR methods, Real Time PCR, to hybrid capture and microarrays has been proposed [ 6 , [19] , [20] , [21] ].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR

et al. 2018

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“…This, however, is not unexpected because sex-specific traits of panarthropods regularly develop after embryogenesis during post-embryonic or even post-larval developmental stages, or they are difficult to spot on morphological grounds. Examples include the initiation of sex-specific sexual behavior, the Drosophila melanogaster sex combs, the sex brushes of related drosophilids, the bulbi of male spiders, the development of the male phenotype in water fleas, the gonopods in male myriapods, and gonadal differentiation in general ([103] Coddington 1990, [104] Drago et al 2008, [13] Kato et al 2011, [105] Rice et al 2018). In the future, it would be interesting to study expression of Dmrt genes in different sex-specific tissues of larval, juvenile and adult sex-specific structures in order to get a better understanding of the general role these genes may play in sexual differentiation.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis and embryonic expression of panarthropod Dmrt genes

2019

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Background One set of the developmentally important Doublesex and Male-abnormal-3 Related Transcription factors (Dmrt) is subject of intense research, because of their role in sex-determination and sexual differentiation. This likely non-monophyletic group of Dmrt genes is represented by the Drosophila melanogaster gene Doublesex ( Dsx ), the Caenorhabditis elegans Male-abnormal-3 ( Mab-3 ) gene, and vertebrate Dmrt1 genes. However, other members of the Dmrt family are much less well studied, and in arthropods, including the model organism Drosophila melanogaster , data on these genes are virtually absent with respect to their embryonic expression and function. Results Here we investigate the complete set of Dmrt genes in members of all main groups of Arthropoda and a member of Onychophora, extending our data to Panarthropoda as a whole. We confirm the presence of at least four families of Dmrt genes (including Dsx -like genes) in Panarthropoda and study their expression profiles during embryogenesis. Our work shows that the expression patterns of Dmrt11E, Dmrt93B, and Dmrt99B orthologs are highly conserved among panarthropods. Embryonic expression of Dsx -like genes, however, is more derived, likely as a result of neo-functionalization after duplication. Conclusions Our data suggest deep homology of most of the panarthropod Dmrt genes with respect to their function that likely dates back to their last common ancestor. The function of Dsx and Dsx -like genes which are critical for sexual differentiation in animals, however, appears to be much less conserved. Electronic supplementary material The online version of this article (10.1186/s12983-019-0322-0) contains supplementary material, which is available to authorized users.

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“…Moreover, the morpho-anatomical information that emerges from these data provide an important basis for the study of post-embryonic expression patterns of genes putatively involved this kind of metamorphosis, as suggested by Drago et al [6]. …”

Section: Discussionmentioning

confidence: 99%

Structural aspects of leg-to-gonopod metamorphosis in male helminthomorph millipedes (Diplopoda)

et al. 2011

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BackgroundIn the adult males of helminthomorph millipedes, one or two pairs of legs in the anterior part of the trunk are strongly modified into sexual appendages (gonopods) used for sperm transfer during the copula. Gonopods differentiate in an advanced phase of post-embryonic development, in most cases as replacement for the walking legs of the seventh trunk ring, as these first regress to tiny primordia, to eventually develop into gonopods at a subsequent stadium. These extremely localized but dramatic changes have been described as a non-systemic metamorphosis. In the present study we describe morphological and anatomical changes of trunk ring VII associated with non-systemic metamorphosis in four helminthomorph species.ResultsAs documented here for the first time by means of traditional histology methods and new techniques based on confocal laser scanning microscopy, the external modifications caused by non-systemic metamorphosis are associated to a huge rearrangement of internal anatomy, mostly due to the development of gonopod apodemes and extrinsic muscles.ConclusionsInternal changes in the seventh trunk ring, locally leading to the dorsal displacement of the ventral nerve cord and the digestive tract, are modulated in a taxon-specific manner, and are very conspicuous in the blaniulids Nopoiulus kochii and Blaniulus guttulatus, with likely major functional consequences.

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Non-systemic metamorphosis in male millipede appendages: long delayed, reversible effect of an early localized positional marker?

Cited by 15 publications

References 10 publications

Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR

Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR

Phylogenetic analysis and embryonic expression of panarthropod Dmrt genes

Structural aspects of leg-to-gonopod metamorphosis in male helminthomorph millipedes (Diplopoda)

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