Non‐surgical embryo transfer technique and recipient mare pregnancy rate

Card, Claire

doi:10.1136/vr.k3700

Cited by 1 publication

(1 citation statement)

References 13 publications

(31 reference statements)

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“…Embryo transfer equipment for rhinoceros cows could involve using Wilsher forceps developed for the non-surgical mare ET ( Card 2018 ) to fixate and straighten the cervix and a specially designed semi-rigid catheter to overcome the long folded cervix through which a smaller diameter ET catheter may be passed to dispose fresh or frozen–thawed embryos. In the elephant, a minimally invasive access through the cranial part of the vestibulum as described previously for AI ( Schmitt 2006 ) may be an option to ensure access to the cervix and placement of the embryo into the ipsilateral horn of ovulation.…”

Section: Advanced Art – Future Perspectivesmentioning

confidence: 99%

Comparative reproduction of the female horse, elephant and rhinoceros: implications for advancing assisted reproductive technologies

Meuffels-Barkas,

Wilsher,

Allen

et al. 2023

Reproduction and Fertility

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Recent loss of rhinoceros subspecies has renewed interest in using more advanced assisted reproductive technologies (ART) in rhinoceroses and elephants. Currently, only semen collection, semen preservation and artificial insemination (AI) have been used repeatedly with success in these species. Although ovum pick-up (OPU) and intra-cytoplasmic sperm injection (ICSI) have been reported in rhinoceroses, the techniques are not yet optimised. In contrast, multiple ART applications are routinely used in the horse. Since elephant and rhinoceroses share some reproductive features with equids, we postulate that procedures such as OPU, ICSI, in vitro fertilisation (IVF) and embryo transfer (ET), which are well established in the horse, may represent a basis to develop protocols for endangered pachyderms. In this review, we summarize current knowledge on reproductive physiology relevant to ART. We discuss the current state of ART in all three families and the requirements for the successful implementation of OPU, ICSI, IVF and ET in these species.

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Section: Advanced Art – Future Perspectivesmentioning

confidence: 99%