Non-structure protein ORF1ab (NSP8) in SARS-CoV-2 contains potential γδT cell epitopes

Du, Bin; Guo, Yang; Li, Gang; Zhu, Yinian; Wang, Yunfu; Xi, Xueyan

doi:10.3389/fmicb.2022.936272

Cited by 2 publications

(2 citation statements)

References 36 publications

(41 reference statements)

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“…39 Transfected Vd2 TCRs from COVID-19 patients activated in response to epitopes that were present on non-structural protein 8 (NSP8), a protein encoded in the SARS-CoV-2 genome. 40 However, we did not find any evidence of disease severity being associated with expansions of the similar TCRs in COVID-19. We showed that CD27 À Vd2 effector T cell proportions correlated with disease severity.…”

Section: Discussioncontrasting

confidence: 66%

“…In addition, another study confirmed these results and also showed that these cells had enhanced cytolytic potential, killed influenza‐infected cells (A and B) and utilized a public Vγ9 TCR CDR3 paired to various Vδ2 CDR3s 39 . Transfected Vδ2 TCRs from COVID‐19 patients activated in response to epitopes that were present on non‐structural protein 8 (NSP8), a protein encoded in the SARS‐CoV‐2 genome 40 . However, we did not find any evidence of disease severity being associated with expansions of the similar TCRs in COVID‐19.…”

Section: Discussionmentioning

confidence: 70%

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Circulating effector γδ T cell populations are associated with acute coronavirus disease 19 in unvaccinated individuals

et al. 2023

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Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection causes severe coronavirus disease 2019 (COVID‐19) in a small proportion of infected individuals. The immune system plays an important role in the defense against SARS‐CoV‐2, but our understanding of the cellular immune parameters that contribute to severe COVID‐19 disease is incomplete. Here, we show that populations of effector γδ T cells are associated with COVID‐19 in unvaccinated patients with acute disease. We found that circulating CD27negCD45RA+CX3CR1+ Vδ1effector cells expressing Granzymes (Gzms) were enriched in COVID‐19 patients with acute disease. Moreover, higher frequencies of GzmB+ Vδ2+ T cells were observed in acute COVID‐19 patients. SARS‐CoV‐2 infection did not alter the γδ T cell receptor repertoire of either Vδ1+ or Vδ2+ subsets. Our work demonstrates an association between effector populations of γδ T cells and acute COVID‐19 in unvaccinated individuals.

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Section: Discussioncontrasting

confidence: 66%

Section: Discussionmentioning

confidence: 70%

Circulating effector γδ T cell populations are associated with acute coronavirus disease 19 in unvaccinated individuals

et al. 2023

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Longitudinal dynamic single-cell mass cytometry analysis of peripheral blood mononuclear cells in COVID-19 patients within 6 months after viral RNA clearance

Zhou,

Zhao,

et al. 2024

BMC Infect Dis

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This study investigates the longitudinal dynamic changes in immune cells in COVID-19 patients over an extended period after recovery, as well as the interplay between immune cells and antibodies. Leveraging single-cell mass spectrometry, we selected six COVID-19 patients and four healthy controls, dissecting the evolving landscape within six months post-viral RNA clearance, alongside the levels of anti-spike protein antibodies. The T cell immunophenotype ascertained via single-cell mass spectrometry underwent validation through flow cytometry in 37 samples. Our findings illuminate that CD8 + T cells, gamma-delta (gd) T cells, and NK cells witnessed an increase, in contrast to the reduction observed in monocytes, B cells, and double-negative T (DNT) cells over time. The proportion of monocytes remained significantly elevated in COVID-19 patients compared to controls even after six-month. Subpopulation-wise, an upsurge manifested within various T effector memory subsets, CD45RA + T effector memory, gdT, and NK cells, whereas declines marked the populations of DNT, naive and memory B cells, and classical as well as non-classical monocytes. Noteworthy associations surfaced between DNT, gdT, CD4 + T, NK cells, and the anti-S antibody titer. This study reveals the changes in peripheral blood mononuclear cells of COVID-19 patients within 6 months after viral RNA clearance and sheds light on the interactions between immune cells and antibodies. The findings from this research contribute to a better understanding of immune transformations during the recovery from COVID-19 and offer guidance for protective measures against reinfection in the context of viral variants.

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Non-structure protein ORF1ab (NSP8) in SARS-CoV-2 contains potential γδT cell epitopes

Cited by 2 publications

References 36 publications

Circulating effector γδ T cell populations are associated with acute coronavirus disease 19 in unvaccinated individuals

Circulating effector γδ T cell populations are associated with acute coronavirus disease 19 in unvaccinated individuals

Longitudinal dynamic single-cell mass cytometry analysis of peripheral blood mononuclear cells in COVID-19 patients within 6 months after viral RNA clearance

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