Non-structural carbohydrate status and CO2 exchange rate of apple fruitlets at the time of abscission influenced by shade, NAA or BA

Stopar, M.; Resnik, M.; Pongrac, Vida Žnidaršič

doi:10.1016/s0304-4238(00)00169-2

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…A significant increase of Suc concentration in abscising fruitlets with respect to the NAFs was also found (Fig. 6) promptly after the treatment, as demonstrated previously by Stopar et al (2001). Accumulation of Suc is often reported as a reaction to sugar starvation and has generally been considered to be an adaptive response to the stress condition (Roitsch, 1999).…”

Section: Selection Of De Genes In the Seedsupporting

confidence: 79%

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

et al. 2010

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Apple (Malus 3 domestica) represents an interesting model tree crop for studying fruit abscission. The physiological fruitlet drop occurring in this species can be easily magnified by using thinning chemicals, such as benzyladenine (BA), to obtain fruits with improved quality and marketability. Despite the economic importance of this process, the molecular determinants of apple fruitlet abscission are still unknown. In this research, BA was used to obtain fruitlet populations with different abscission potentials to be analyzed by means of a newly released 30K oligonucleotide microarray. RNAs were extracted from cortex and seed of apple fruitlets sampled over a 4-d time course, during which BA triggers fruit drop, and used for microarray hybridization. Transcriptomic profiles of persisting and abscising fruitlets were tested for statistical association with abscission potential, allowing us to identify molecular signatures strictly related to fruit destiny. A hypothetical model for apple fruitlet abscission was obtained by putting together available transcriptomic and metabolomic data. According to this model, BA treatment would establish a nutritional stress within the tree that is primarily perceived by the fruitlet cortex whose growth is blocked by resembling the ovary growth inhibition found in other species. In weaker fruits, this stress is soon visible also at the seed level, likely transduced via reactive oxygen species/sugar and hormones signaling cross talk, and followed by a block of embryogenesis and the consequent activation of the abscission zone.

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Section: Selection Of De Genes In the Seedsupporting

confidence: 79%

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

et al. 2010

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“…In addition, another significant long-term effect of auxin was the repression of fruit energy metabolism. The auxin-mediated restriction on respiration has been observed in apple [ 58 ] and loquat fruitlets [ 59 ], but reports examining the direct effects of auxin on energy metabolism are still lacking. Based on our transcriptome sequencing results, the inhibition of energy metabolism by auxin was also a long-term effect.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive RNA-Seq Analysis on the Regulation of Tomato Ripening by Exogenous Auxin

Tao

et al. 2016

PLoS ONE

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Auxin has been shown to modulate the fruit ripening process. However, the molecular mechanisms underlying auxin regulation of fruit ripening are still not clear. Illumina RNA sequencing was performed on mature green cherry tomato fruit 1 and 7 days after auxin treatment, with untreated fruit as a control. The results showed that exogenous auxin maintained system 1 ethylene synthesis and delayed the onset of system 2 ethylene synthesis and the ripening process. At the molecular level, genes associated with stress resistance were significantly up-regulated, but genes related to carotenoid metabolism, cell degradation and energy metabolism were strongly down-regulated by exogenous auxin. Furthermore, genes encoding DNA demethylases were inhibited by auxin, whereas genes encoding cytosine-5 DNA methyltransferases were induced, which contributed to the maintenance of high methylation levels in the nucleus and thus inhibited the ripening process. Additionally, exogenous auxin altered the expression patterns of ethylene and auxin signaling-related genes that were induced or repressed in the normal ripening process, suggesting significant crosstalk between these two hormones during tomato ripening. The present work is the first comprehensive transcriptome analysis of auxin-treated tomato fruit during ripening. Our results provide comprehensive insights into the effects of auxin on the tomato ripening process and the mechanism of crosstalk between auxin and ethylene.

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“…Fruit is a very strong metabolic sink, and fruit set is tightly related to the availability of carbohydrates [ 7 , 8 , 38 ]. Shortage of carbohydrates resulting in excessive fruit drop has been reported in apple [ 39 , 40 ], citrus [ 41 ], and longan [ 42 ]. CPPU has been demonstrated to regulate carbohydrate allocation [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of CPPU on Carbohydrate and Endogenous Hormone Levels in Young Macadamia Fruit

Zeng

Yang

et al. 2016

PLoS ONE

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N-(2-Chloro-4-pyridyl)-N′-phenylurea (CPPU) is a highly active cytokinin-like plant growth regulator that promotes chlorophyll biosynthesis, cell division, and cell expansion. It also increases fruit set and accelerates fruit enlargement. However, there has been no report about the effect of CPPU on fruit development and its physiological mechanism in macadamia. In this study, we investigated the effect of CPPU treatment at early fruit development via foliar spray or raceme soaking at 20 mg·L-1 on fruit set and related physiology in macadamia. Changes in carbohydrate contents and endogenous hormones in leaves, bearing shoots and fruit were also examined. Results showed that CPPU significantly reduced young fruit drop and delayed the wave of fruit drop by 1–2 weeks. The treatment significantly decreased the contents of total soluble sugars and starch in the leaves, but increased them in the bearing shoots and total soluble sugars in the husk (pericarp) and seeds. These findings suggested that CPPU promoted carbohydrate mobilization from the leaves to the fruit. In addition, CPPU increased the contents of indole-3-acetic acid (IAA), gibberellin acid (GA3), and zeatin riboside (ZR) and decreased the abscisic acid (ABA) in the husk. Therefore, CPPU treatment reduced the early fruit drop by increasing carbohydrate availability and by modifying the balance among endogenous hormones.

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Non-structural carbohydrate status and CO2 exchange rate of apple fruitlets at the time of abscission influenced by shade, NAA or BA

Cited by 12 publications

References 27 publications

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

Comprehensive RNA-Seq Analysis on the Regulation of Tomato Ripening by Exogenous Auxin

Effect of CPPU on Carbohydrate and Endogenous Hormone Levels in Young Macadamia Fruit

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