Non‐steroidal and steroidal anti‐inflammatory drugs vary in their modulation of dendritic cell function in the elicitation phase of allergic contact dermatitis

Abstract

The role of dendritic cells (DCs) in allergic contact dermatitis has been clearly demonstrated for the induction phase. However, the situation during the elicitation phase is very complex within a distinct inflammatory response. This study was performed to exploit DC migration in the elicitation phase in a mouse model of allergic contact dermatitis and to evaluate the effects of steroidal and non-steroidal anti-inflammatory drugs (NSAIDs) on DC migration through skin in the elicitation phase of allergic contact dermatitis. Topically and systemically administered acetylsalicylic acid (ASA) did not reduce the inflammatory response. However, systemically administered ASA significantly reduced the DC migration to the draining lymph node. In contrast, topically administered indomethacin reduced the inflammatory response, but had only minor effects on DC migration, whereas diflorasone diacetate reduced both inflammatory reaction and DC migration. Thus, NSAIDs may differ in their inhibitory action in immunological inflammation.

The selective glucocorticoid receptor agonist mapracorat displays a favourable safety–efficacy ratio for the topical treatment of inflammatory skin diseases in dogs

“…The observation that glucocorticoids mediate only a partial inhibition of LPS‐induced cytokine release from murine BMDC is corroborated by former findings, where the highly potent topical steroid (WHO class 1) diflorasone diacetate was tested in the same system, leading to similar maximal inhibitory potential .…”

Postelicitation model of allergic contact dermatitis for predicting the efficacy of topical drugs

“…Moreover, these naturally occurring compounds in UV‐exposed skin most probably lack any serious side‐effect, in contrast to corticosteroids and several NSAIDs. Interestingly, a study revealed that the immunological mechanisms of anti‐inflammation by corticosteroids in the elicitation phase differ from non‐steroidal drugs (4), such as the imidazolic drug candidates.…”

“…NSAIDs). To that end, mice are sensitized with contact sensitizers, such as 2,4,6‐trinitrochlorobenzene (picryl chloride)(1), 2,4‐dinitrofluorobenzene (2), toluene‐2,4‐diisocyanate (3,4) or 4‐ethoxymethylen‐2‐phenyl‐2‐oxazolin‐5‐one (oxazolone)(5), or p‐phenylenediamine or its metabolites, which are the most frequently encountered contact sensitizers in humans (6). Here, in this study, the frequently used contact sensitizer oxazolone was used.…”

Oral administration of paeoniflorin attenuates allergic contact dermatitis by inhibiting dendritic cell migration and Th1 and Th17 differentiation in a mouse model

“…The cartilage-free dorsal halves of mouse ear skin at 24 h after the third sensitization with DNCB were split and cultured in 24-well plates as previously described with minor revisions [23,24]. The mouse ear skin was placed onto a table made of sieves with the epidermal side facing upwards.…”