Non-specific adsorption of protein to microfluidic materials

Rashid, Sabaa; Ward-Bond, Jesse; Krupin, Oleksiy; Berini, Pierre

doi:10.1016/j.colsurfb.2021.112138

Cited by 9 publications

(6 citation statements)

References 20 publications

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“…In this work, Bovine Serum Albumin (BSA) was used as a nonspecific protein model because it can easily attack many materials through nonspecific interactions [ 47 ]. Figure 2 a shows the effect of the length of the PEG midblock on anti-nonspecific adsorption.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the optimal synthesis conditions of AmZPP x were as follows: molecular weight of PEG midblock, 5000; DA-PEG 5000 -DA concentration, 0.2 mM; and contact time, 30 min. In this work, Bovine Serum Albumin (BSA) was used as a nonspecific protein model because it can easily attack many materials through nonspecific interactions [47]. Figure 2a shows the effect of the length of the PEG midblock on anti-nonspecific adsorption.…”

Section: Synthesis Of Amzpp Xmentioning

confidence: 99%

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Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

et al. 2023

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Immobilized angiotensin-converting enzyme (ACE) is a promising material for the rapid screening of antihypertensive drugs, but the nonspecific adsorption is a serious problem in separation processes involving complex biological products. In this study, triblock copolymers with dopamine (DA) block as anchors and PEG block as the main body (DA-PEGx-DA) were attached to an immobilized ACE (ACE@mZIF-8/PDA, AmZP) surface via the “grafting to” strategy which endowed them with anti-nonspecific adsorption. The influence of DA-PEGx-DA chain length on nonspecific adsorption was confirmed. The excellent specificity and reusability of the obtained ACE@mZIF-8/PDA/DA-PEG5000-DA (AmZPP5000) was validated by screening two known ACE inhibitory peptides Val-Pro-Pro (VPP, competitive inhibitory peptides of ACE) and Gly-Met-Lys-Cys-Ala-Phe (GF-6, noncompetitive inhibitory peptides of ACE) from a mixture containing active and inactive compounds. These results demonstrate that anchored polymer loops are effective for high-recognition selectivity and AmZPP5000 is a promising compound for the efficient separation of ACE inhibitors in biological samples.

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Section: Resultsmentioning

confidence: 99%

Section: Synthesis Of Amzpp Xmentioning

confidence: 99%

Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

et al. 2023

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“…The contact angle of Fe 3 O 4 @SiO 2 –ILI-01 was only 15.90°. BSA is of high abundance (roughly 10 mg/mL) in cell culture media and is commonly used as the indicator to investigate the surface adsorption characteristics . Thus, the nonspecific adsorption of Fe 3 O 4 @SiO 2 –ILI-01 was evaluated with BSA (10 mg/mL).…”

Section: Results and Discussionmentioning

confidence: 99%

“…BSA is of high abundance (roughly 10 mg/mL) in cell culture media and is commonly used as the indicator to investigate the surface adsorption characteristics. 32 Thus, the nonspecific adsorption of Fe 3 O 4 @SiO 2 −ILI-01 was evaluated with BSA (10 mg/mL). As shown in Figure S6a, after three washes with 1 mL of washing buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.0005% Tween 20, 2 mM CaCl 2 ), the remaining BSA in the supernatant was unnoticeable in the bands of SDS-PAGE.…”

Section: ■ Introductionmentioning

confidence: 99%

High Throughput and Noninvasive Exosomal PD-L1 Detection for Accurate Immunotherapy Response Prediction via Tim4-Functionalized Magnetic Core–Shell Metal–Organic Frameworks

Wang,

Liu,

Zhang

et al. 2023

Anal. Chem.

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“…As a sticky protein, BSA was often used as a simulated pollutant for ultrafiltration membrane antifouling tests. 67 The results of the three-cycle filtration of BSA solution and pure water are presented in Figure 7a. The membranes were washed by pure water for 1 h after each cycle of the 3 cycles.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Full-Coverage Spongy HEAA/PES Composite Ultrafiltration Membrane with High Selectivity and Antifouling Performances

Chen

Zhu

Chen

et al. 2023

ACS Appl. Polym. Mater.

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Selectivity and antifouling of ultrafiltration (UF) membranes are major challenges in the separation and purification of biological proteins. This work reported that full-coverage spongy poly(ether sulfone) (PES) ultrafiltration membranes assisted by the self-cross-linking of N-hydroxyethyl acrylamide (HEAA) were fabricated via a phase inversion method at room temperature. The pore structure of the UF membrane from finger-like to coverage of the spongy structure occurred by increasing the content of HEAA, which is beneficial for size-selective separation of biomolecules. The optimized M4 membrane showed a high surface porosity with a uniform pore size of 30 nm, resulting in more than 99% protein rejection rate for immunoglobulin G (IgG) but less than 20% rejection for bovine serum albumin, confirming that the molecular weight cutoff is 100k Da. Furthermore, the fabricated PES composite ultrafiltration membranes showed excellent antifouling performance and flux recovery efficiency for proteins due to the hydrophilic group of HEAA. This study provides an intelligent strategy for fouling prevention and selective isolation of proteins.

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Non-specific adsorption of protein to microfluidic materials

Cited by 9 publications

References 20 publications

Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

Anchoring of Polymer Loops on Enzyme-Immobilized Mesoporous ZIF-8 Enhances the Recognition Selectivity of Angiotensin-Converting Enzyme Inhibitory Peptides

High Throughput and Noninvasive Exosomal PD-L1 Detection for Accurate Immunotherapy Response Prediction via Tim4-Functionalized Magnetic Core–Shell Metal–Organic Frameworks

Full-Coverage Spongy HEAA/PES Composite Ultrafiltration Membrane with High Selectivity and Antifouling Performances

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