Non-specific adhesion on biomaterial surfaces driven by small amounts of protein adsorption

Understanding protein adsorption phenomena at solid surfaces

“…On artificial tissue scaffolds protein adsorption is the key factor for a proper vascularization [6] whereas on biomedical implants that are in contact with the blood stream protein adsorption can lead to thrombosis [7,8]. Further, protein adsorption can trigger adhesion of particles, bacteria or cells possibly promoting inflammation cascades, or fouling processes [9][10][11][12]. In the field of analytical sciences non-specific protein adsorption on sensor surfaces, protein chips, or assay platforms is a serious problem degrading the analytical performance of the device [13].…”

Deposition of colloid particles on protein layers: Fibrinogen on mica

“…Precise kinetic measurements of fibrinogen adsorption on silicon and modified glass surfaces forming parallel-plate channels were performed using the in situ fluorescent TIRF technique by Santore and co-workers [11,14,15]. These results were supplemented with an interesting AFM determination of the kinetics of fibrinogen adsorption for a low coverage range.…”

Development of proteomic tools to study protein adsorption on a biomaterial, titanium grafted with poly(sodium styrene sulfonate)

“…Development of this strategy imply (a) to demonstrate the efficiency of the polyNaSS surface to protein adsorption, (b) to pre-treat the polyNaSS/Ti surface with various protein samples and testing them on cell response, and (c) to identify proteins adsorbed on the more responsive pre-treated polyNaSS/Ti surface. To analyse proteins adsorbed on biomaterial, different techniques such as radiolabeling [15], ellipsometry [16,17], surface plasmon resonance (SPR) sensor [18][19][20], enzyme immunoassaying [21], gold-colloid-labelled immunoassay combined with micro-bicinchoninic acid protein assay [22] have been used. However none of them led to the identification of adsorbed proteins.…”