Non-selective cation channels in basolateral-membrane vesicles from pars recta of rabbit kidney proximal tubule

Blokkebak-Poulsen, Jens; Sheikh, Mujeeb; Jacobsen, Christian

doi:10.1042/bj2720839

Cited by 3 publications

(3 citation statements)

References 18 publications

(20 reference statements)

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“…In previous papers we have demonstrated K ÷ and Na ÷ ion channels in membrane vesicles isolated from outer cortex (pars convoluta) and outer medulla (pars recta) of rabbit proximal tubule [6][7][8][9].…”

Section: Introductionmentioning

confidence: 94%

GTP‐binding proteins in luminal and basolateral membranes from pars convoluta and pars recta of rabbit kidney proximal tubule

et al. 1992

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The GTP-binding proteins on luminal and basolateral membrane vesicles from outer cortex (pars convoluta) and outer medulla (pars recta) of rabbit proximal tubule have b~n examined. The membrane vesicles were highly purified, as ascertained by electron microscopy, by measurements of marker enzymes, and by investigating seganental-specifie transport systems. The [~SJGTP~,S binding to vesicles, and to sodium cholatc-cxtracted proteins from vesicles, indicated that the total content of GTP-binding proteins were equally distributed on pars convoluta, pars recta luminal and basolateral membranes. The m~mbranes were ADP-ribosylated with [~:P]NAD" in the presence of pertussis toxin and cholera toxin. Gel elec~ro-phoresis revealed, for all preparations, the presence of cholera toxin [~ZP]ADP.ribosylated 42 and 45 kDa Gct, proteins, and pertussis toxin [~:P]ADP-ribosylated 41 kDa G~, 40 kDa G0::. and 41 kDa G~ proteins. The 2D electrophoresis indicated that G,,'s were not present in luminal nor in basolateral membranes of pars convoluta or pars recta of rabbit proximal tubule. G-protein; Proximal tubule

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Section: Introductionmentioning

confidence: 94%

GTP‐binding proteins in luminal and basolateral membranes from pars convoluta and pars recta of rabbit kidney proximal tubule

et al. 1992

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“…In the proximal tubule several K + channels, a non-selective ion channel, and an amiloride sensitive Na + channel have been described [3,6,8,9,17,24].…”

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confidence: 99%

“…The functional role of the nonselective cation channel is not clear [3]. The various K + channels contribute to the maintenance of a hyperpolarized membrane voltage, and experimental evidence has accumulated that at least one kind of K ÷ channel is pH regulated, and another one may be regulated by Ca 2+.…”

mentioning

confidence: 99%

Regulation of tubular transport via ion channels

1993

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Hydrostatic pressure-regulated ion transport in bladder uroepithelium

Wang

Lee

Johnson

et al. 2003

American Journal of Physiology-Renal Physiology

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The effect of hydrostatic pressure on ion transport in the bladder uroepithelium was investigated. Isolated rabbit uroepithelium was mounted in modified Ussing chambers and mechanically stimulated by applying hydrostatic pressure across the mucosa. Increased hydrostatic pressure led to increased mucosal-to-serosal Na+ absorption across the uroepithelium via the amiloride-sensitive epithelial Na+ channel. In addition to this previously characterized pathway for Na+ absorption, hydrostatic pressure also induced the secretion of Cl- and K+ into the mucosal bathing solution under short-circuit conditions, which was confirmed by a net serosal-to-mucosal flux of 36Cl- and 86Rb+. K+ secretion was likely via a stretch-activated nonselective cation channel sensitive to 100 microM amiloride, 10 mM tetraethylammonium, 3 mM Ba2+, and 1 mM Gd3+. Hydrostatic pressure-induced ion transport in the uroepithelium may play important roles in electrolyte homeostasis, volume regulation, and mechanosensory transduction.

show abstract

Non-selective cation channels in basolateral-membrane vesicles from pars recta of rabbit kidney proximal tubule

Cited by 3 publications

References 18 publications

GTP‐binding proteins in luminal and basolateral membranes from pars convoluta and pars recta of rabbit kidney proximal tubule

GTP‐binding proteins in luminal and basolateral membranes from pars convoluta and pars recta of rabbit kidney proximal tubule

Regulation of tubular transport via ion channels

Hydrostatic pressure-regulated ion transport in bladder uroepithelium

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