Non-scanning motionless fluorescence three-dimensional holographic microscopy

Rosen, Joseph; Brooker, Gary

doi:10.1038/nphoton.2007.300

Cited by 385 publications

(265 citation statements)

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“…Similarly, various point-of-care diagnostic devices have been developed and among them optical imaging and sensing techniques are highly advantageous as they can provide real-time, highresolution and highly sensitive quantitative information, potentially assisting rapid and accurate diagnosis. [30][31][32][33][34][35][36][37][38][39][40] To date, a number of optical techniques have been proposed for point-of-care diagnostics such as in vitro optical devices, [41][42][43][44][45][46][47][48][49][50][51][52][53] including portable optical imaging systems, optical microscopes integrated to cell phones or in vivo optical devices, [54][55][56][57][58][59][60][61][62][63] involving confocal microscopy, microendoscopy and optical coherence tomography techniques. Among these approaches, lens-free computational on-chip imaging 64 has been an emerging technique that can eliminate the need for bulky and costly optical components while also preserving (or even enhancing in certain cases) the image resolution, field of view and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Handheld high-throughput plasmonic biosensor using computational on-chip imaging

et al. 2014

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We demonstrate a handheld on-chip biosensing technology that employs plasmonic microarrays coupled with a lens-free computational imaging system towards multiplexed and high-throughput screening of biomolecular interactions for point-of-care applications and resource-limited settings. This lightweight and field-portable biosensing device, weighing 60 g and 7.5 cm tall, utilizes a compact optoelectronic sensor array to record the diffraction patterns of plasmonic nanostructures under uniform illumination by a single-light emitting diode tuned to the plasmonic mode of the nanoapertures. Employing a sensitive plasmonic array design that is combined with lens-free computational imaging, we demonstrate label-free and quantitative detection of biomolecules with a protein layer thickness down to 3 nm. Integrating large-scale plasmonic microarrays, our on-chip imaging platform enables simultaneous detection of protein mono-and bilayers on the same platform over a wide range of biomolecule concentrations. In this handheld device, we also employ an iterative phase retrieval-based image reconstruction method, which offers the ability to digitally image a highly multiplexed array of sensors on the same plasmonic chip, making this approach especially suitable for high-throughput diagnostic applications in field settings.

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Section: Introductionmentioning

confidence: 99%

Handheld high-throughput plasmonic biosensor using computational on-chip imaging

et al. 2014

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“…This review concentrates on the technique of incoherent digital holography based on single-channel incoherent interferometers, which we have been involved in their development recently [16][17][18][19] . The type of hologram discussed here is the digital Fresnel hologram, which means that a hologram of a single point has the form of the well-known FZP.…”

Section: General Properties Of Fresnel Hologramsmentioning

confidence: 99%

“…The discussed incoherent digital hologram is dubbed Fresnel incoherent correlation hologram (FINCH) [16][17][18] . The FINCH is actually based on a single channel onaxis incoherent interferometer.…”

Section: General Properties Of Fresnel Hologramsmentioning

confidence: 99%

“…Various aspects of the FINCH have been described in Refs. [16][17][18][19], including FINCH of reflected white light 16 , FINCH of fluorescence objects 17 , a FINCH-based holographic fluorescence microscope 18 and finally a hologram recorder in a mode of a synthetic aperture 19 . We briefly review these works in the current section.…”

Section: Fresnel Incoherent Correlation Holographymentioning

confidence: 99%

“…Besides a constant term, the hologram expression contains two terms of correlation between an object and a quadratic phase, Z-dependent, function. In order to remain with a single correlation term out of the three terms, we follow the usual procedure of on-axis digital holography 14,[16][17][18][19] . Three holograms of the same object are recorded each of which with a different phase constant.…”

Section: Fresnel Incoherent Correlation Holographymentioning

confidence: 99%

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Review of three-dimensional holographic imaging by Fresnel incoherent correlation holograms

Rosen

Katz

Brooker

2010

3D Res

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Holographic imaging offers a reliable and fast method to capture the complete 3-D information of the scene from a single perspective. We review our recently proposed single-channel optical system for generating digital Fresnel holograms of 3-D real-existing objects illuminated by incoherent light. In this motionless holographic technique, light is reflected, or emitted, from a 3-D object, propagates through a spatial light modulator (SLM), and is recorded by a digital camera. The SLM is used as a beam-splitter of the single-channel incoherent interferometer, such that each spherical beam originated from each object point is split into two spherical beams with two different curve radiuses. Incoherent sum of the entire interferences between all the couples of spherical beams creates the Fresnel hologram of the observed 3-D object. When this hologram is reconstructed in the computer, the 3D properties of the object are revealed.

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