Non-scanning CARS microscopy using wide-field geometry

Toytman, I.; Cohn, Keith; Smith, T. I.; Simanovskii, D.; Palanker, Daniel

doi:10.1117/12.701617

Cited by 3 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the major competitor to point-wise illumination approachs, a number of wide-field CARS 3 4 5 6 7 configurations were reported receiving information of a multitude of microscopic sample locations while distributing the excitation power over a 3D volume in the range of several micrometers in every direction. Due to the narrow directional distribution of excitation k-vectors offered, the reported configurations require samples with negligible axial extension.…”

mentioning

confidence: 99%

Bessel beam CARS of axially structured samples

Heuke

Zheng

Akimov

et al. 2015

Sci Rep

View full text Add to dashboard Cite

We report about a Bessel beam CARS approach for axial profiling of multi-layer structures. This study presents an experimental implementation for the generation of CARS by Bessel beam excitation using only passive optical elements. Furthermore, an analytical expression is provided describing the generated anti-Stokes field by a homogeneous sample. Based on the concept of coherent transfer functions, the underling resolving power of axially structured geometries is investigated. It is found that through the non-linearity of the CARS process in combination with the folded illumination geometry continuous phase-matching is achieved starting from homogeneous samples up to spatial sample frequencies at twice of the pumping electric field wave. The experimental and analytical findings are modeled by the implementation of the Debye Integral and scalar Green function approach. Finally, the goal of reconstructing an axially layered sample is demonstrated on the basis of the numerically simulated modulus and phase of the anti-Stokes far-field radiation pattern.

show abstract

mentioning

confidence: 99%

Bessel beam CARS of axially structured samples

Heuke

Zheng

Akimov

et al. 2015

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Because vibrational modes of molecules are dependent on chemical bonds and local molecular arrangements, vibrational spectroscopies reveal molecular fingerprints, the mapping of which produces label-free imaging modalities, such as coherent anti-Stokes Raman spectroscopy (CARS) [1][2][3][4][5][6], stimulated Raman scattering [7], spontaneous Raman [8,9], and Fourier-transform infrared absorption spectroscopy (IRAS) [10][11][12][13][14][15][16][17][18][19]. However, the spatial resolution of these methods is restricted by diffraction to values between λ and λ/2.…”

Section: Introductionmentioning

confidence: 99%

A framework for far-field infrared absorption microscopy beyond the diffraction limit

Silien¹,

Liu²,

Hendaoui³

et al. 2012

Opt. Express

View full text Add to dashboard Cite

A framework is proposed for infrared (IR) absorption microscopy in the far-field with a spatial resolution below the diffraction limit. The sub-diffraction resolution is achieved by pumping a transient contrast in the population of a selected vibrational mode with IR pulses that exhibit alternating central minima and maxima, and by probing the corresponding absorbance at the same wavelength with adequately delayed Gaussian pulses. Simulations have been carried out on the basis of empirical parameters emulating patterned thin films of octadecyltrichlorosilane and a resolution of 250 nm was found when probing the CH 2 stretches at 3.5 μm with pump energies less than ten times the vibrational saturation threshold. with video-rate coherent anti-Stokes Raman scattering microscopy," Proc. Natl. Acad. Sci. U.S.A. 102(46), 16807-16812 (2005). 5. M. Balu, G. Liu, Z. Chen, B. J. Tromberg, and E. O. Potma, "Fiber delivered probe for efficient CARS imaging of tissues," Opt. Express 18(3), 2380-2388 (2010). 6. I. Toytman, K. Cohn, T. Smith, D. Simanovskii, and D. Palanker, "Non-scanning CARS microscopy using widefield geometry," Proc. SPIE 6442, 64420D, 64420D-7 (2007). 7. C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, "Labelfree biomedical imaging with high sensitivity by stimulated Raman scattering microscopy," Science 322(5909), 1857-1861 (2008 ©2012 Optical Society of America

show abstract

“…Among those, optical vibrational spectroscopies such as coherent anti-Stokes Raman spectroscopy (CARS) [1][2][3][4][5][6], stimulated Raman scattering [7], spontaneous Raman [8,9], and Fourier-transform infrared absorption spectroscopy (IRAS) [10-20] reveal molecular fingerprints because the vibrational modes of molecules are dependent on chemical bonds and local molecular arrangements. Thus label-free imaging is readily achieved by mapping spatially localized spectra.…”

Section: Introductionmentioning

confidence: 99%

High resolution imaging with differential infrared absorption micro-spectroscopy

et al. 2013

View full text Add to dashboard Cite

Although confocal infrared (IR) absorption micro-spectroscopy is well established for far-field chemical imaging, its scope remains restricted since diffraction limits the spatial resolution to values a little above half the radiation wavelength. Yet, the successful implementations of below-the-diffraction limit far-field fluorescence microscopies using saturated irradiation patterns for example for stimulated-emission depletion and saturated structured-illumination suggest the possibility of using a similar optical patterning strategy for infrared absorption mapping at high resolution. Simulations are used to show that the simple mapping of the difference in transmitted/reflected IR energy between a saturated vortexshaped beam and a Gaussian reference with a confocal microscope affords the generation of high-resolution vibrational absorption images. On the basis of experimentally relevant parameters, the simulations of the differential absorption scheme reveal a spatial resolution better than a tenth of the wavelength for incident energies about a decade above the saturation threshold. The saturated structured illumination concepts are thus expected to be compatible with the establishment of point-like point-spread functions for measuring the absorbance of samples with a scanning confocal microscope recording the differential transmission/reflection.

show abstract

Non-scanning CARS microscopy using wide-field geometry

Cited by 3 publications

References 11 publications

Bessel beam CARS of axially structured samples

Bessel beam CARS of axially structured samples

A framework for far-field infrared absorption microscopy beyond the diffraction limit

High resolution imaging with differential infrared absorption micro-spectroscopy

Contact Info

Product

Resources

About