Non-rapid eye movement sleep determines resilience to social stress

Bush, Brittany J; Donnay, Caroline; Andrews, Eva-Jeneé A; Lewis-Sanders, Darielle; Gray, Cloe L; Qiao, Zhimei; Brager, Allison J.; Johnson, Hadiya; Brewer, Hamadi; Sood, Sahil; Saafir, Talib; Benveniste, Morris; Paul, Ketema N.; Ehlen, J. Christopher

doi:10.7554/elife.80206

Cited by 11 publications

(7 citation statements)

References 63 publications

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“…Measures beyond gross sleep-wake state measures may reveal more nuanced and biologically relevant changes in the sleep researcher’s experiments. As we show using the Bush and colleague’s dataset, [12,13] their pharmacogenetic treatment increased NREMS bouts, but not duration. These differences can mark important differences between various psychiatric and neurological diseases.…”

Section: Discussionmentioning

confidence: 90%

SleepInvestigatoR: A flexible R function for analyzing scored sleep in rodents

Gamble,

Williams,

McKenna

et al. 2024

Preprint

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Analyzing scored sleep is a fundamental prerequisite to understanding how sleep changes between health and disease. Classically, this is accomplished by manually calculating various measures (e.g., percent of non-rapid eye movement sleep) from a collection of scored sleep files. This process can be tedious and error prone especially when studies include a large number of animals or involve long recording sessions. To address this issue, we present SleepInvestigatoR, a versatile tool that can quickly organize and analyze multiple scored sleep files into a single output. The function is written in the open-source statistical language R and has a total of 25 parameters that can be set to match a wide variety of experimenter needs. SleepInvestigatoR delivers a total of 22 unique measures of sleep, including all measures commonly reported in the rodent literature. A simple plotting function is also provided to quickly graph and visualize the scored data. All code is designed to be implemented with little formal coding knowledge and step-by-step instructions are provided on the corresponding GitHub page. Overall, SleepInvestigatoR provides the sleep researcher a critical tool to increase efficiency, interpretation, and reproducibility in analyzing scored rodent sleep.

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Section: Discussionmentioning

confidence: 90%

SleepInvestigatoR: A flexible R function for analyzing scored sleep in rodents

Gamble,

Williams,

McKenna

et al. 2024

Preprint

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“…NREMs are linked to the brain’s function in clearing Aβ. Conversely, stress causes fragmentation of NREMs, disrupts sleep continuity and can lead to cognitive impairment and anxiety ( Benkirane et al, 2022 ; Bush et al, 2022 ). In our study, increased NREM duration after SDS may be a key factor in relieving anxiety.…”

Section: Discussionmentioning

confidence: 99%

Activation of ventral tegmental area vesicular GABA transporter (Vgat) neurons alleviates social defeat stress-induced anxiety in APP/PS1 mice

Yao

Li²,

Kora³

et al. 2023

Front. Aging Neurosci.

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IntroductionAlzheimer’s disease (AD) is a progressive neurodegenerative disease that results in cognitive impairment and is often accompanied by anxiety. In this study, we investigated whether the activation of VTAVgat neurons could reduce anxiety in APP/PS1 mice. We hypothesized that acute social defeat stress (SDS) would lead to anxiety in APP/PS1 mice, and that the activation of VTAVgat neurons would alleviate this anxiety.MethodsWe exposed APP/PS1 mice to acute SDS and assessed anxiety using the open field test and elevated plus-arm test. Activated VTAVgat neurons was tested by cfos staining. Sleep quality was detected using electroencephalogram after SDS or non-SDS procedure. Sleep duration, sleep latency, and non-rapid eye movement (NREM) percentage were analyzed. VTAVgat neurons were chemogenetically activated by deschloroclozapine.ResultsOur results showed that acute SDS led to anxiety in APP/PS1 mice, as evidenced by increased anxiety-related behaviors in the open field and elevated plus-arm tests. Activation of VTAVgat neurons by SDS led to an increase in sleep duration, primarily due to a decrease in sleep latency and an increase in NREMs. However, the quality of sleep was poor. Chemogenetical activation of VTAVgat neurons improved sleep quality and relieved SDS-induced anxiety. Furthermore, the anxiety state correlated negatively with sleep duration and NREM percentage and correlated positively with theta power density in APP/PS1 mice.DiscussionOur study provides evidence that the activation of VTAVgat neurons alleviates SDS-induced anxiety in APP/PS1 mice, suggesting that poor sleep quality may exacerbate anxiety in AD. These findings may have important implications for the treatment of anxiety in AD, as targeting VTAVgat neurons could be a potential therapeutic approach.

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“…Implantation was performed with the animals under pentobarbital anesthesia (50 mg/kg, i.p.). C57BL/6N mice were also chosen for recording and data analysis through EEG surgery, following established procedures and methods in previous reports [29,30]. To enable electroencephalogram (EEG) measurements, the head mount was precisely placed with its front edge 3.0 mm anterior to the bregma.…”

Section: Eeg Surgery Recording and Data Analysismentioning

confidence: 99%

Aralia continentalis Root Enhances Non-Rapid Eye Movement Sleep by Activating GABAA Receptors

Yoon,

Lim,

Jung

et al. 2023

Nutrients

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Aralia continentalis exhibits various biological activities; however, their sleep-promoting effects have not been previously reported. In this study, we evaluated the hypnotic effects and sleep–wake profiles of A. continentalis root (KS-126) using a pentobarbital-induced sleep-acceleration test and polysomnographic recordings. Additionally, we investigated the molecular mechanism of KS-126 through patch-clamp electrophysiology. Our polysomnographic recordings revealed that KS-126 not only accelerated the onset of non-rapid eye movement sleep (NREMS) but also extends its duration. Considering the temporal dynamics of the sleep–wake stages, during the initial and subsequent periods KS-126 extended NREMS duration and decreased wakefulness, thereby enhancing sleep-promoting effects. Furthermore, the assessment of sleep quality via analysis of electroencephalogram power density indicated that KS-126 did not significantly alter sleep intensity. Finally, we found that KS-126 enhanced GABAA receptor-mediated synaptic responses in primary hippocampal neurons, leading to an increase in the percentage of the GABA current. This effect was not affected by the selective benzodiazepine receptor antagonist flumazenil, but was entirely inhibited by the GABAA receptor antagonist bicuculline. In conclusion, KS-126 extends the duration of NREMS without altering its intensity by prolonging GABAergic synaptic transmission, which modulates GABAA receptor function.

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Non-rapid eye movement sleep determines resilience to social stress

Cited by 11 publications

References 63 publications

SleepInvestigatoR: A flexible R function for analyzing scored sleep in rodents

SleepInvestigatoR: A flexible R function for analyzing scored sleep in rodents

Activation of ventral tegmental area vesicular GABA transporter (Vgat) neurons alleviates social defeat stress-induced anxiety in APP/PS1 mice

Aralia continentalis Root Enhances Non-Rapid Eye Movement Sleep by Activating GABAA Receptors

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