Non-radioactive, isomer-specific inositol phosphate mass determinations: high-performance liquid chromatography-micro-metal-dye detection strongly improves speed and sensitivity of analyses from cells and micro-enzyme assays

Guse, Andreas H.; Goldwich, Andreas; Weber, Karin; Mayr, Georg W.

doi:10.1016/0378-4347(95)00219-9

Cited by 45 publications

(45 citation statements)

References 19 publications

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“…NAADP mediated Ca 2ϩ release starts rapidly within a few seconds upon TCR/CD3 ligation (8). By contrast, InsP 3 -and cADPRmediated releases appear within a few minutes (39) or within tens of minutes, respectively (6). A possible reason for this complex regulatory Ca 2ϩ release network may be the need for temporal checkpoints, at which the T cell, based upon the input via the TCR/CD3 complex and diverse co-receptors, can control whether to proceed with activation or not (40).…”

Section: Discussionmentioning

confidence: 99%

NAADP-mediated Ca ²⁺ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist

Dammermann

Zhang

Nebel

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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105

159

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Section: Discussionmentioning

confidence: 99%

NAADP-mediated Ca ²⁺ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist

Dammermann

Zhang

Nebel

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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105

159

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“…These results might be explained by the following model: upon stimulation of T cells through the TCR/CD3 complex, Ins(1,4,5)P3 is rapidly formed (Guse et al, 1995) and cADPR increases more slowly (Guse et al, 1999). Opening of Ins(1,4,5)P3R close to the site of Ins(1,4,5)P3 production results in local Ca 2+ signals; expression of Ins(1,4,5)P3R is crucial for this process as knockdown of the type 1 Ins(1,4,5)P3R prevented the activation of global Ca 2+ signaling in Jurkat T cells (Jayaraman et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Amplification and propagation of pacemaker Ca2+ signals by cyclic ADP-ribose and the type 3 ryanodine receptor in T cells

Kunerth

Langhorst

Schwarzmann

et al. 2004

Journal of Cell Science

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Ligation of the T-cell receptor/CD3 complex results in global Ca2+ signals that are essential for T-cell activation. We have recently reported that these global Ca2+ signals are preceded by localized pacemaker Ca2+ signals. Here, we demonstrate for the first time for human T cells that an increase in signal frequency of subcellular pacemaker Ca2+ signals at sites close to the plasma membrane, in the cytosol and in the nucleus depends on the type 3 ryanodine receptor (RyR) and its modulation by cyclic ADP-ribose. The spatial distribution of D-myo-inositol 1,4,5-trisphosphate receptors and RyRs indicates a concerted action of both of these receptors/Ca2+ channels in the generation of initial pacemaker signals localized close to the plasma membrane. Inhibition or knockdown of RyRs resulted in significant decreases in (1) the frequency of initial pacemaker signals localized close to the plasma membrane, and (2) the frequency of localized pacemaker Ca2+ signals in the inner cytosol. Moreover, upon microinjection of cyclic ADP-ribose or upon extracellular addition of its novel membrane-permeant mimic N-1-ethoxymethyl-substituted cyclic inosine diphosphoribose, similarly decreased Ca2+ signals were observed in both type 3 RyR-knockdown cells and in control cells microinjected with the RyR antagonist Ruthenium Red. Taken together, our results show that, under physiological conditions in human T cells, RyRs play crucial roles in the local amplification and the spatiotemporal development of subcellular Ca2+ pacemaker signals.

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“…(c) Techniques for the separation of inositol phosphates (i) Liquid chromatography Several methods have been used to separate inositol phosphates, including ion-exchange (high performance liquid chromatography (HPLC) or conventional largecolumn ion-exchange) and size exclusion/gel filtration (table 3), although most have involved ion-exchange chromatography to isolate the individual congeners (Tangendjaja et al 1980;Camire & Clydesdale 1982;Graf & Dintzis 1982;Batty et al 1985;Wilson et al 1985;Cilliers & Van Niekerk 1986;Meek & Nicoletti 1986;Portilla & Morrison 1986;Horstman et al 1988;Matthews et al 1988;Mayr 1988;Minear et al 1988;Phillipy & Bland 1988;Heathers et al 1989;Taylor et al 1990;Bos et al 1991;Prestwich & Bolton 1991;Clarkin et al 1992;Rounds & Nielsen 1993;Wolters et al 1993;Nanny et al 1994Nanny et al , 1995Guse 1995;Suzumura & Kamatani 1995b). The ability of anion-exchange HPLC to separate the various inositol phosphate congeners effectively and efficiently has led to its widespread use.…”

Section: Analytical Techniques For the Determination Of Inositol Phosmentioning

confidence: 99%

Inositol phosphates in the environment

Turner¹,

Paphazy

Haygarth³

et al. 2002

Phil. Trans. R. Soc. Lond. B

675

554

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The inositol phosphates are a group of organic phosphorus compounds found widely in the natural environment, but that represent the greatest gap in our understanding of the global phosphorus cycle. They exist as inositols in various states of phosphorylation (bound to between one and six phosphate groups) and isomeric forms (e.g. myo, d-chiro, scyllo, neo), although myo-inositol hexakisphosphate is by far the most prevalent form in nature. In terrestrial environments, inositol phosphates are principally derived from plants and accumulate in soils to become the dominant class of organic phosphorus compounds. Inositol phosphates are also present in large amounts in aquatic environments, where they may contribute to eutrophication. Despite the prevalence of inositol phosphates in the environment, their cycling, mobility and bioavailability are poorly understood. This is largely related to analytical difficulties associated with the extraction, separation and detection of inositol phosphates in environmental samples. This review summarizes the current knowledge of inositol phosphates in the environment and the analytical techniques currently available for their detection in environmental samples. Recent advances in technology, such as the development of suitable chromatographic and capillary electrophoresis separation techniques, should help to elucidate some of the more pertinent questions regarding inositol phosphates in the natural environment.

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Non-radioactive, isomer-specific inositol phosphate mass determinations: high-performance liquid chromatography-micro-metal-dye detection strongly improves speed and sensitivity of analyses from cells and micro-enzyme assays

Cited by 45 publications

References 19 publications

NAADP-mediated Ca ²⁺ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist

NAADP-mediated Ca ²⁺ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist

Amplification and propagation of pacemaker Ca2+ signals by cyclic ADP-ribose and the type 3 ryanodine receptor in T cells

Inositol phosphates in the environment

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Non-radioactive, isomer-specific inositol phosphate mass determinations: high-performance liquid chromatography-micro-metal-dye detection strongly improves speed and sensitivity of analyses from cells and micro-enzyme assays

Cited by 45 publications

References 19 publications

NAADP-mediated Ca 2+ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist

NAADP-mediated Ca 2+ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist

Amplification and propagation of pacemaker Ca2+ signals by cyclic ADP-ribose and the type 3 ryanodine receptor in T cells

Inositol phosphates in the environment

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NAADP-mediated Ca ²⁺ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist

NAADP-mediated Ca ²⁺ signaling via type 1 ryanodine receptor in T cells revealed by a synthetic NAADP antagonist