Non-protein coding RNA-based genosensor with quantum dots as electrochemical labels for attomolar detection of multiple pathogens

Vijian, Dinesh; Chinni, Suresh V.; Yin, Lee Su; Lertanantawong, Benchaporn; Surareungchai, Werasak

doi:10.1016/j.bios.2015.10.057

Cited by 56 publications

(23 citation statements)

References 45 publications

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“…QDs labeling offers the potential to develop multiplexed determination architectures, based on the separated oxidation potentials of the different QD tags [17,38,67,68,69,70]. On the other hand, metal NP-labeled voltammetric transducers are not able to perform multi-analyte electrochemical bioassays in a single run applying different NP labels.…”

Section: Multiplexed Detection Of Dna Sequences Exploiting Nps As mentioning

confidence: 99%

“…A smart biosensor for the simultaneous detection of sequences of Vibrio cholerae , Salmonella sp., and Shigella sp., developed by Vijian et al [67]. CdS, ZnS, and PbS QDs conjugated with DNA probes that were specific to each target analyte were used as labels and sandwich-hybridization assays were applied (Figure 8).…”

Section: Multiplexed Detection Of Dna Sequences Exploiting Nps As mentioning

confidence: 99%

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Electrochemical DNA Biosensors Based on Labeling with Nanoparticles

Kokkinos

2019

Nanomaterials

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This work reviews the field of DNA biosensors based on electrochemical determination of nanoparticle labels. These labeling platforms contain the attachment of metal nanoparticles (NPs) or quantum dots (QDs) on the target DNA or on a biorecognition reporting probe. Following the development of DNA bioassay, the nanotags are oxidized to ions, which are determined by voltammetric methods, such as pulse voltammetry (PV) and stripping voltammetry (SV). The synergistic effects of NPs amplification (as each nanoprobe releases a large number of detectable ions) and the inherent sensitivity of voltammetric techniques (e.g., thanks to the preconcentration step of SV) leads to the construction of ultrasensitive, low cost, miniaturized, and integrated biodevices. This review focuses on accomplishments in DNA sensing using voltammetric determination of nanotags (such as gold and silver NPs, and Cd- and Pb-based QDs), includes published works on integrated three electrode biodevices and paper-based biosystems, and discusses strategies for multiplex DNA assays and signal enhancement procedures. Besides, this review mentions the electroactive NP synthesis procedures and their conjugation protocols with biomolecules that enable their function as labels in DNA electrochemical biosensors.

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Section: Multiplexed Detection Of Dna Sequences Exploiting Nps As mentioning

confidence: 99%

Section: Multiplexed Detection Of Dna Sequences Exploiting Nps As mentioning

confidence: 99%

Electrochemical DNA Biosensors Based on Labeling with Nanoparticles

Kokkinos

2019

Nanomaterials

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“…and Shigella sp. ; with high potential for monitoring multiple pathogens in environmental samples [120]. …”

Section: Enhancing Performancementioning

confidence: 99%

Molecular Techniques for the Detection of Organisms in Aquatic Environments, with Emphasis on Harmful Algal Bloom Species

Medlin

Orozco

2017

Sensors

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Molecular techniques to detect organisms in aquatic ecosystems are being gradually considered as an attractive alternative to standard laboratory methods. They offer faster and more accurate means of detecting and monitoring species, with respect to their traditional homologues based on culture and microscopic counting. Molecular techniques are particularly attractive when multiple species need to be detected and/or are in very low abundance. This paper reviews molecular techniques based on whole cells, such as microscope-based enumeration and Fluorescence In-Situ Hybridization (FISH) and molecular cell-free formats, such as sandwich hybridization assay (SHA), biosensors, microarrays, quantitative polymerase chain reaction (qPCR) and real time PCR (RT-PCR). Those that combine one or several laboratory functions into a single integrated system (lab-on-a-chip) and techniques that generate a much higher throughput data, such as next-generation systems (NGS), were also reviewed. We also included some other approaches that enhance the performance of molecular techniques. For instance, nano-bioengineered probes and platforms, pre-concentration and magnetic separation systems, and solid-phase hybridization offer highly pre-concentration capabilities. Isothermal amplification and hybridization chain reaction (HCR) improve hybridization and amplification techniques. Finally, we presented a study case of field remote sensing of harmful algal blooms (HABs), the only example of real time monitoring, and close the discussion with future directions and concluding remarks.

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“…A reporter nanomaterial is a nanomaterial that can be used as electrochemical, colorimetric, fluorescent, or other types of signal molecule. Metal NPs [ 29 ], metallic oxide NPs [ 30 , 31 ] and QDs [ 32 , 33 ] are known to function as electrochemical reporter (stripping voltammetry). On the other hand, metal nanoclusters [ 34 , 35 ], QDs [ 36 , 37 ] and up-conversion NPs [ 38 ] can emit fluorescence that can influenced by quencher, change in structure or environment [ 39 ].…”

Section: Different Functional Roles Of Nanomaterials In Food Safetmentioning

confidence: 99%

Recent Progresses in Nanobiosensing for Food Safety Analysis

Yang

Huang

Zhu

et al. 2016

Sensors

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With increasing adulteration, food safety analysis has become an important research field. Nanomaterials-based biosensing holds great potential in designing highly sensitive and selective detection strategies necessary for food safety analysis. This review summarizes various function types of nanomaterials, the methods of functionalization of nanomaterials, and recent (2014–present) progress in the design and development of nanobiosensing for the detection of food contaminants including pathogens, toxins, pesticides, antibiotics, metal contaminants, and other analytes, which are sub-classified according to various recognition methods of each analyte. The existing shortcomings and future perspectives of the rapidly growing field of nanobiosensing addressing food safety issues are also discussed briefly.

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Non-protein coding RNA-based genosensor with quantum dots as electrochemical labels for attomolar detection of multiple pathogens

Cited by 56 publications

References 45 publications

Electrochemical DNA Biosensors Based on Labeling with Nanoparticles

Electrochemical DNA Biosensors Based on Labeling with Nanoparticles

Molecular Techniques for the Detection of Organisms in Aquatic Environments, with Emphasis on Harmful Algal Bloom Species

Recent Progresses in Nanobiosensing for Food Safety Analysis

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