Non-programmable cryopreservation of sperm from industrial-farmed Murray cod (Maccullochella p peelii)

Liu, Meijian; Yuan, Sheng; Zhao, Zi-Ming; Liu, Ming; Lin, Apeng; Gong, Qian

doi:10.1016/j.aquaculture.2021.736811

Cited by 2 publications

(1 citation statement)

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“…Other recent studies have surprisingly achieved higher fertilisation rates in cryopreserved sperm than expected through fresh sperm using relatively fast cooling rates [103; Appendix 4]. Moderate cooling rates over liquid nitrogen [16] can be achieved with simple Styrofoam apparatus [203,318]. The cooling rate depends on the height of the straws above the liquid nitrogen, the material of the apparatus and box, and how long the LN2 is in the container before sperm freezing.…”

Section: Appendix 3 Sperm Cryopreservationmentioning

confidence: 99%

Ecological Civilization COP 15 and Amphibian Sustainability through Reproduction Technologies, Biobanking, and Conservation Breeding Programs (RBCs)

Browne,

Luo,

Wang

et al. 2023

Preprint

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Intergenerational Justice entitles the maximum retention of Earth’s Biodiversity. The United Nations COP 15 committed to the protection of 30% of Earth’s terrestrial environments to provide Biospheric Sustainability. However, proactive amphibian sustainability needs support from amphibian Reproduction Technologies, Biobanking, and Conservation Breeding Programs [RBCs]. These technologies include the reliable practice of stimulation of reproduction and the collection of sperm and oocytes, refrigerated and cryopreserved sperm storage, in vitro fertilization. Emerging technologies promise the perpetuation of species solely from biobanked biomaterials. However, although exemplary national programs for amphibian RBCs are now established in the USA and Australia, these potentials are not fully realised resulting in unacceptable biodiversity loss. To fully realise these potentials an independent and democratic global organisation is proposed to, 1) focus on the bioregions with the highest amphibian biodiversity, 2) maximise regional inclusion in international initiatives, and 3) provide for maximum community engagement and financial resources. We present the first overall multidisciplinary review of amphibian RBCs, including their geopolitical, cultural, and scientific background, along with their development and application to economically, efficiently, and reliably, perpetuate amphibian biodiversity. We provide genetic models, the potential for diploid cell cryopreservation to perpetuate species, along with significant new information on methodologies and techniques, and standardise nomenclature for specificity in publications and media consistency.

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Section: Appendix 3 Sperm Cryopreservationmentioning

confidence: 99%