Non-photochemical quenching in four unicellular algae with different light-harvesting and xanthophyll-cycle pigments

Casper-Lindley, Catharina; Björkman, Olle

doi:10.1007/978-94-011-3953-3_534

Cited by 57 publications

(89 citation statements)

References 7 publications

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“…The de-epoxidation state of the xanthophyll cycle pigments, given by the de-epoxidation ratio DT:(DD þ DT) is typically correlated with nonphotochemical quenching (Casper-Lindley & Bjorkman, 1998). However, we found that SternVolmer coefficient SV m increased continuously throughout the 36 h following the shift from 50 to 800 mmol photons m À2 s…”

Section: Xanthophyll Cycle In E Huxleyimentioning

confidence: 57%

Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Harris

Scanlan

Geider

2009

European Journal of Phycology

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A non-calcifying strain (Ply 92) of Emiliania huxleyi (Lohman) Hay et Mohler, was subjected to reciprocal step changes of photon flux density (PFD) between 50 and 800 mmol photons m À2 s À1 to establish the timescales of photoacclimation.Photoacclimation in E. huxleyi involved adjustment of cellular light harvesting pigment contents, but not cellular ribulose bisphosphate carboxylase-oxygenase (RuBisCO) or cellular RNA contents. There was considerable variability in both the magnitude and the rate of the response of physiological and biophysical variables to the step changes in PFD. The slowest response was observed in cellular chl a content, and the chl a/carbon ratio. Acclimation of these variables appeared to be due to dilution of pigment pools rather than to pigment turnover. Changes in the chl a-specific light absorption coefficient accompanied changes in cellular chl a content. Following the shifts in PFD, the efficiency of excitation energy transfer from the photosystem II antennae to reaction centres II (RCII), as assessed from F Changes in non-photochemical excitation energy quenching were correlated with the relative and absolute abundance of the xanthophyll cycle pigments diadinoxanthin and diatoxanthin, which appeared to be reversibly interconverted with fucoxanthin. Thus, acclimation to PFD involved co-ordinated adjustment of the quantum efficiency of RCII, the efficiency of excitation energy transfer from the light-harvesting pigment bed to RCII, and cellular light absorption on overlapping time scales.

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Section: Xanthophyll Cycle In E Huxleyimentioning

confidence: 57%

Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Harris

Scanlan

Geider

2009

European Journal of Phycology

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“…Maximum F v /F m reported for green algae is around 0.83, approaching values measured in higher plants, whereas F v /F m of diatoms usually does not exceed 0.75 (e.g. Bu¨chel & Wilhelm, 1993;Geel et al, 1997;Casper-Lindley & Bjo¨rkman, 1998;Flameling & Kromkamp, 1998;Koblı´zˇek et al, 2001). Nevertheless, values for F v /F m approaching 0.8 are occasionally reported for diatoms in field samples (Villareal, 2004) as well as in cultures (Forster & Martin-Je´ze´quel, 2005).…”

Section: Field Measurementsmentioning

confidence: 97%

Photosynthetic characteristics of the phytoplankton in the Scheldt estuary: community and single-cell fluorescence measurements

Dijkman

Kromkamp

2006

European Journal of Phycology

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Estuaries present a very dynamic environment for phytoplankton with large changes in salinity occurring over relatively short distances, usually with high turbidity. We investigated the photosynthetic characteristics of phytoplankton in the Scheldt estuary (Belgium and the Netherlands) along a salinity gradient using community and single-cell fluorescence measurements. Single-cell fluorescence measurements offer the opportunity to study differences between species that were formerly possible only using uni-algal cultures. The objective was to study differences in the photochemical efficiency of photosystem II (F v /F m ) between different species or different genera (when identification to the species level was not possible) and to investigate the effect of salinity on individual species. The difference in F v /F m between species was small, but often significant (ANOVA, p < 0.05), and was found to be a true characteristic of species, as opposed to being caused by stochastic processes affecting cells of all species more or less equally. High algal mortality is implied by a large decline of biomass from freshwater towards higher salinity, as well as a complete change in the phytoplankton composition. Nevertheless, cells with low F v /F m , which would indicate decreased health, were rare. F v /F m of 93% of the measured cells was between 0.5 and 0.8. There was no obvious relation between F v /F m and species abundance. In laboratory studies, green algae are often reported to have a higher F v /F m than diatoms, but we found no indication of this in the Scheldt estuary.

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“…The amplitude and kinetics of the whole NPQ process and the importance of each component (Fig. 3b) can be extremely divergent between taxa, especially among microalgal groups (Casper-Lindley and Bjorkman 1998;Hill et al 2005;Juneau and Harrison 2005), and even between species within a taxonomic group Lavaud et al 2007). For example, qE shows high amplitude and fast onset in diatoms and brown macroalgae, while being of minor importance in most of the green microalgae (Finazzi et al 2006) and cyanobacteria (Kirilovsky 2007).…”

Section: Effect Of Light Stress On Fluorescence Signatures and Their mentioning

confidence: 99%

Fluorescence as a Tool to Understand Changes in Photosynthetic Electron Flow Regulation

Ralph

Wilhelm

Lavaud

et al. 2010

Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications

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Non-photochemical quenching in four unicellular algae with different light-harvesting and xanthophyll-cycle pigments

Cited by 57 publications

References 7 publications

Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Photosynthetic characteristics of the phytoplankton in the Scheldt estuary: community and single-cell fluorescence measurements

Fluorescence as a Tool to Understand Changes in Photosynthetic Electron Flow Regulation

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