Limestone aquifers provide the main drinking
water resources of southern Italy. Due to cattle grazing
and/or manure spreading, these aquifers are often characterized
by microbial contamination of groundwater. The
aim of this paper is to summarize the results obtained
during a 10-year research carried out in experimental field
sites in southern Italy, analyzing (1) the influence of the
topsoil of pyroclastic origin on the migration of microbial
cells from the ground towards the groundwater, and then on
the groundwater vulnerability, (2) the influence of the
rainfall regime on the breakthrough at the springs, (3) the
reliability of thermotolerant coliforms and fecal enterococci
as bacterial indicators of microbial contamination,
and (4) the effectiveness of microorganisms as natural
tracers for some hydrogeological purposes. The results
obtained showed that fecal enterococci are a more reliable
indicator than thermotolerant coliforms for detecting contamination
and that the entity and distribution over time of
microbial contamination of fecal origin are influenced by
several factors, such as precipitation regime, thermal
regime and existence and thickness of the topsoil of
pyroclastic origin. Moreover, the migration of a significant
amount of bacterial cells through the topsoil and the
underlying carbonate rocks allows the utilization of
microorganisms as effective natural tracers, to be coupled
with other classic tracers to study the recharge and the flow
processes. In a broader perspective, these results can be
used to optimize the investigations in other hydrogeological
scenarios, with emphasis on those where different water
types coexist and interact in same aquifer systems