Non-periodic oscillatory deformation of an actomyosin microdroplet encapsulated within a lipid interface

Nishigami, Yukinori; Ito, Hiroaki; Sato, Seiji; Ichikawa, Masatoshi

doi:10.1038/srep18964

Cited by 18 publications

(10 citation statements)

References 45 publications

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“…1C). Cup shapes are reminiscent of the ones of buckled spherical capsules under an osmotic shock (5,9) and the wrinkled shapes are reminiscent of that of a liposome under acto-myosin contraction (19,20). Again, we confirm that naked liposomes subjected to a decrease in volume (osmotic shock) display dynamical thermal shape undulations (Supplementary movie 2).…”

Section: Effect Of An Osmotic Shock On Liposomes Coated Withsupporting

confidence: 68%

Actin shells control buckling and wrinkling of biomembranes

et al. 2019

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Section: Effect Of An Osmotic Shock On Liposomes Coated Withsupporting

confidence: 68%

Actin shells control buckling and wrinkling of biomembranes

et al. 2019

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“…Using the toolbox of synthetic biology, several studies have shown the internal dynamics of the cytoskeleton leading to a contraction or deformation of the cell rim. [8][9][10][11] The autonomous motion of the entire minimal cell, however, is more difficult to achieve. For example, autonomous motion was obtained via reconstitution of fluid flows initiated by kinesin activity and collective dynamics of microtubule bundles.…”

Section: Introductionmentioning

confidence: 99%

Autonomous Directional Motion of Actin‐Containing Cell‐Sized Droplets

Haller

Jahnke

Weiss

et al. 2020

Advanced Intelligent Systems

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Cell motility is potentially the most apparent distinction of living matter, serving an essential purpose in single cells and multicellular organisms alike. Thus, the bottom‐up reconstitution of autonomous motion of cell‐sized compartments remains an exciting but challenging goal. Herein, actin‐driven Marangoni flows are engineered to generate rotational and translational motility of surfactant‐stabilized emulsion droplets. The interaction between actin filaments and the negatively charged block‐copolymer Krytox is identified as the driving force for Marangoni flows at the droplet interface. Tuning the actin–Krytox interplay, sustained autonomous unidirectional droplet rotation with 1.7 rot h−1 is achieved. Ultimately, this rotational motion is transformed into a translational rolling motion by introducing interactions between the droplets and the surface of the observation chamber. Accordingly, translational motility of actin‐containing droplets at velocities of 0.061 ± 0.014 μm s−1 is reported herein and an overall displacement of several hundreds of micrometers within 30 min is observed. These self‐propelled systems with biologically active molecules demonstrate how motility could be implemented for synthetic cells.

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“…Besides being potentially relevant to explain cell swimming in bulk, our model may also serve as a framework to design contractility-powered self-motile synthetic actomyosin droplets in the lab (50). Directly testable predictions of our work include the speedup of motion with increasing contractility and stiffness and the emergence of circular motion in bulk.…”

Section: Discussionmentioning

confidence: 84%

Actomyosin Contraction Induces In-Bulk Motility of Cells and Droplets

Goff

Liebchen

Marenduzzo

2020

Biophysical Journal

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Cell crawling on two-dimensional surfaces is a relatively well-understood phenomenon that is based on actin polymerization at a cell's front edge and anchoring on a substrate, allowing the cell to pull itself forward. However, some cells, such as cancer cells invading a three-dimensional matrigel, can also swim in the bulk, where surface adhesion is impossible. Although there is strong evidence that the self-organized engine that drives cells forward in the bulk involves myosin, the specific propulsion mechanism remains largely unclear. Here, we propose a minimal model for in-bulk self-motility of a droplet containing an isotropic and compressible contractile gel, representing a cell extract containing a disordered actomyosin network. In our model, contraction mediates a feedback loop between myosin-induced flow and advection-induced myosin accumulation, which leads to clustering and locally enhanced flow. The symmetry of such flow is then spontaneously broken through actomyosin-membrane interactions, leading to self-organized droplet motility relative to the underlying solvent. Depending on the balance between contraction, diffusion, detachment rate of myosin, and effective surface tension, this motion can be either straight or circular. Our simulations and analytical results shed new light on in-bulk myosin-driven cell motility in living cells and provide a framework to design a novel type of synthetic active matter droplet potentially resembling the motility mechanism of biological cells.

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Non-periodic oscillatory deformation of an actomyosin microdroplet encapsulated within a lipid interface

Cited by 18 publications

References 45 publications

Actin shells control buckling and wrinkling of biomembranes

Actin shells control buckling and wrinkling of biomembranes

Autonomous Directional Motion of Actin‐Containing Cell‐Sized Droplets

Actomyosin Contraction Induces In-Bulk Motility of Cells and Droplets

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