Non‐pathogenic association of L‐form bacteria<i>(pseudomonas syringae pv. phaseolicola)</i>with bean plants<i>(Phaseolus vulgaris</i>L.) and its potential for biocontrol of halo blight disease

Amijee, F.; Allans, E.J.; Waterhouse, Rosemary N.; Glover, Lesley Anne; Paton, Alan

doi:10.1080/09583159209355234

Cited by 27 publications

(35 citation statements)

References 11 publications

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“…However, streptomycetes can also be forced to produce single cells. L-forms have been shown to associate with plants acting as biocontrol agents (Amijee, Allans, Waterhouse, Glover, & Paton, 1992;Innes & Allan, 2001). While protoplasts cannot propagate, streptomycetes can also form so-called L-forms (Innes & Allan, 2001).…”

Section: A Special Case: Streptomyces L-formsmentioning

confidence: 99%

“…While protoplasts cannot propagate, streptomycetes can also form so-called L-forms (Innes & Allan, 2001). Biocontrol activity was shown for Pseudomonas and Bacillus species (Amijee et al, 1992;Walker, Ferguson, Booth, & Allan, 2002;Waterhouse, Buhariwalla, Bourn, Rattray, & Glover, 1996), but could also be true for streptomycetes, which naturally produce a large arsenal of antifungal and antimicrobial compounds (Hopwood, 2007). Subsequent cultivations in osmotically balanced media can lead to the acquisition of mutations that allow these cells to propagate without their cell wall, even in the absence of the inducing agents (i.e., penicillin and lysozyme; Innes & Allan, 2001;Leaver, Dominguez-Cuevas, Coxhead, Daniel, & Errington, 2009;Mercier, Kawai, & Errington, 2013;Errington, 2013).…”

Section: A Special Case: Streptomyces L-formsmentioning

confidence: 99%

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Morphogenesis of Streptomyces in Submerged Cultures

Dissel

Claessen

Wezel

2014

Advances in Applied Microbiology

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Members of the genus Streptomyces are mycelial bacteria that undergo a complex multicellular life cycle and propagate via sporulation. Streptomycetes are important industrial microorganisms, as they produce a plethora of medically relevant natural products, including the majority of clinically important antibiotics, as well as a wide range of enzymes with industrial application. While development of Streptomyces in surface-grown cultures is well studied, relatively little is known of the parameters that determine morphogenesis in submerged cultures. Here, growth is characterized by the formation of mycelial networks and pellets. From the perspective of industrial fermentations, such mycelial growth is unattractive, as it is associated with slow growth, heterogeneous cultures, and high viscosity. Here, we review the current insights into the genetic and environmental factors that determine mycelial growth and morphology in liquid-grown cultures. The genetic factors include cell-matrix proteins and extracellular polymers, morphoproteins with specific roles in liquid-culture morphogenesis, with the SsgA-like proteins as well-studied examples, and programmed cell death. Environmental factors refer in particular to those dictated by process engineering, such as growth media and reactor set-up. These insights are then integrated to provide perspectives as to how this knowledge can be applied to improve streptomycetes for industrial applications.

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Section: A Special Case: Streptomyces L-formsmentioning

confidence: 99%

Section: A Special Case: Streptomyces L-formsmentioning

confidence: 99%

Morphogenesis of Streptomyces in Submerged Cultures

Dissel

Claessen

Wezel

2014

Advances in Applied Microbiology

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“…1994). In particular, bacterial L‐forms have been shown to have the potential to act as plant biocontrol agents (Amijee et al . 1992; Waterhouse et al .…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, the Ps. syringae L‐form/bean symbiosis evoked a plant response to the biocontrol agent indicative of induced resistance (Amijee et al . 1992).…”

Section: Introductionmentioning

confidence: 99%

Induction, growth and antibiotic production of Streptomyces viridifaciens L-form bacteria

Innes

Allan

2001

J Appl Microbiol

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Aims: To induce, cultivate and investigate the characteristics of L-form bacteria derived from the ®lamentous actinomycete Streptomyces viridifaciens. Methods and Results: L-forms were induced in a liquid medium supplemented with lysozyme and penicillin. A stable culture which no longer required inducing agents but could still revert, was obtained by the twelfth subculture. The speci®c growth rate of stable L-forms was faster (0á751) than unstable L-forms (0á361). After the exponential growth phase, the cell diameter continued to increase, as did the percentage of vacuoles. Morphologically, the Lforms appeared as spherical bodies with no signs of differentiation and were sensitive to osmotic stress, indicating removal of the cell wall. The L-forms produced secondary metabolites although much lower levels of antibiotic were assayed in the L-forms compared with the cell walled forms. Conclusions: Stable L-form bacteria were induced from S. viridifaciens and their growth characterized. The L-forms produced secondary metabolites. Signi®cance and Impact of the Study: Stable Streptomyces L-forms were induced and have potential as biocontrol agents.

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“…The first commercial products of this approach are already in the market, but only on a small scale (7,61). Foliar biological control (apart from prevention of ice nucleation, [65]) receives minimal attention today, and very few studies relate to pathovars of P. syringae (4,53). For example, in the last conference of Plant Growth-promoting Rhizobacteria of 1994 in Australia, most of the papers concerned biocontrol agents, yet none out of over 80 studies presented referred to foliar biocontrol.…”

Section: (Iii) Biological Control By Antagonistic Microorganismsmentioning

confidence: 99%

Alternative Strategies for Controlling Plant Diseases Caused by Pseudomonas syringae

Bashan¹

1997

Developments in Plant Pathology

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PLANT DISEASESThe most common strategy for controlling diseases caused by Pseudomonas syringae is, as it has been for more than 5 decades, to spray bactericides. These mainly include a variety of copper compounds or other heavy metals, with or without various combinations of fungicides or other pest-control chemicals. Spraying with antibiotics or other organic bactericides have also been used, but to a lesser extent. Unfortunately, these strategies have never been satisfactory, resulting in heavy crop losses during severe epidemics. Over the last years, pathogenic strains that are resistant to copper spraying have been detected globally and are threatening the continuation of this strategy. Three different strategies are slowly being introduced into the field, (i) seed disinfection by heat or a combination of heat and bactericides, (ii) biological control by antagonistic microorganisms, and (iii) soil solarization especially against pathogens that spend part of their life cycle in the soil. Additional minor strategies include: (i) spraying infested plants with natural-occuring antibacterial compounds, and (ii) various techniques of sustainable agriculture without the use of chemicals (organic farming).

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Non‐pathogenic association of L‐form bacteria(pseudomonas syringae pv. phaseolicola)with bean plants(Phaseolus vulgarisL.) and its potential for biocontrol of halo blight disease

Cited by 27 publications

References 11 publications

Morphogenesis of Streptomyces in Submerged Cultures

Morphogenesis of Streptomyces in Submerged Cultures

Induction, growth and antibiotic production of Streptomyces viridifaciens L-form bacteria

Alternative Strategies for Controlling Plant Diseases Caused by Pseudomonas syringae

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