“Non-parallel Transport” of Enzyme Protein by the Pancreas

Ss, Ratnam

doi:10.1038/213460a0

Cited by 58 publications

(20 citation statements)

References 10 publications

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“…Adelson & Rothman (1974) observed an increase in the ratio of chymotrypsinogen to lipase in the perfused rabbit pancreas stimulated with the peptide chymodenin. Similarly, preferential discharge of trypsinogen over chymotrypsinogen was observed in the in vitro rabbit pancreas stimulated with pancreozymin but not methacholine (Rothman, 1967). Scheele & Palade (1975) however found parallel release of seven secretary enzymes from guinea-pig pancreatic lobules under both basal as well as carbamoylcholine or caruelin stimulated conditions.…”

Section: Resultsmentioning

confidence: 98%

parallel secretion of secretory proteins and calcium by the rat parotid gland.

Kanagasuntheram¹,

Lim²

1981

The Journal of Physiology

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SUMMARY1. The secretion of amylase, deoxyribonuclease, ribonuclease, protein and Ca2+ by the rat parotid gland in vitro was studied.2. Isoproterenol and carbamoyleholine elicited a parallel discharge of amylase, deoxyribonuclease, ribonuclease and protein over a 40 min time period.3. The composition of the secretion was independent of the secretogogue used for stimulation. When gland slices from the same animal were stimulated with isoproterenol, adrenaline, phenylephrine or carbamoyleholine, secretary enzymes and protein were secreted in constant proportions.4. 45Ca injected intraperitoneally 16 h before stimulation with either isoproterenol or carbamoyleholine was released in parallel with amylase and protein.5. The relative proportions of amylase, ribonuclease, deoxyribonuclease, protein and Ca present in isolated parotid gland secretary granules was identical to that of isoproterenol stimulated gland secretion.6. It is concluded that the secretary proteins and Ca2+ are discharged in constant proportions by the rat parotid gland regardless of the mode of stimulation or the rate of secretion. The similarity in the composition of gland secretion and granule contents also suggests that enzymes and Ca2+ are released by exocytosis and not by diffusion across the apical plasma membrane.

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Section: Resultsmentioning

confidence: 98%

parallel secretion of secretory proteins and calcium by the rat parotid gland.

Kanagasuntheram¹,

Lim²

1981

The Journal of Physiology

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“…Besides, most of the experiments that have demonstrated adaptation of the exocrine pancreas to the diet, have mainly been done in anaesthetized animal (LaBella et al, 1981;Tseng et al, 1982) in perfused organs or in vitro preparations of the gland (Rothman, 1967(Rothman, , 1976Adelson & Rothman, 1975;Palade, 1975;Rothman & Wilking, 1978;Dagorn, 1979) whereas experiments in conscious animals, under nearly physiological conditions, are scarce.…”

Section: Discussionmentioning

confidence: 99%

Absence of Rapid Adaptation of the Exocrine Pancreas of Conscious Dogs to Diets Enriched in Fat or Carbohydrates

Mañas¹,

Yago²,

Quiles³

et al. 1996

Archives of Physiology and Biochemistry

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“…Although most experimental evidence suggests that enzymes are secreted in parallel from the pancreas (Tartakoff, Jamieson, Scheele & Palade, 1975), this evidence has been challenged (Rothman, 1975). Non-88P PHYSIOLOGICAL SOCIETY, MARCH 1976 parallel transport from the in-vitro rabbit pancreas was reported following pancreozymin (Vitrum) stimulation, with an increased trypsinogen/chymotrypsinogen ratio, an effect not produced by methacholine (Rothman, 1967). This work has now been repeated using methacholine, pancreozymin (CCK-PZ, GIH Lab., Karolinska Institute, Stockholm) and the C-terminal octa-peptide of pancreozymin (Sincalide, SQ 19,844, E. R. Squibb).…”

Section: Rzsultsmentioning

confidence: 99%

Proceedings of the Physiological Society, 19‐20 March 1976, University College London Meeting: Communications

1976

The Journal of Physiology

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SEl 7EHThe interpretation of the discharge of muscle spindles recorded during normal movements (Cody, Harrison & Taylor, 1975) In cats anaesthetized with chloralose, the superior colliculus-was exposed and, taking its mid point as a reference, the mesencephalic nucleus explored in the rostrocaudal direction, 2-3 mm from the mid line. The electrodes were glass-coated tungsten with 25 gm tip exposed and impedance 5 MD at 1*7 kHz. At positions of maximal unitary response to jaw opening, the recording electrode was used for stimulating with paired stimuli separated by 2 msec repeated every 3 sec. Reflex responses were sought by averaging the e.m.g. in jaw-closing muscles. They were found only when stimulating 1 mm or more caudal to the reference point and increased progressivly towards the caudal end. Excitatory effects could be observed with stimuli as small as 1 V in the caudal part, while rostrally, stimuli up to 30 V were without effect.Thus a limited proportion of the jaw-closing muscle spindles project to their synergist motoneurones, in contrast to the widespread distribution found by Mendell & Henneman (1971) in cat hind limb muscles.

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“Non-parallel Transport” of Enzyme Protein by the Pancreas

Cited by 58 publications

References 10 publications

parallel secretion of secretory proteins and calcium by the rat parotid gland.

parallel secretion of secretory proteins and calcium by the rat parotid gland.

Absence of Rapid Adaptation of the Exocrine Pancreas of Conscious Dogs to Diets Enriched in Fat or Carbohydrates

Proceedings of the Physiological Society, 19‐20 March 1976, University College London Meeting: Communications

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