Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture

Sahu, Neety; Pal, Shilpa; Sapru, Sunaina; Kundu, Joydip; Talukdar, Sarmistha; Singh, Nitu; Yao, Juming; Kundu, Subhas C.

doi:10.1155/2016/7461041

Cited by 33 publications

(26 citation statements)

References 49 publications

(72 reference statements)

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“…For proteins like sericin, different IR wavenumber absorption peaks due to CO stretching vibration of amide I groups represent different major secondary structures. The peak at around 1630 cm −1 was representative of crystalline β‐sheet structures, which was in accordance with previous findings on native sericin structure . Amide I representing absorption bands in the region 1630 and 1660 cm −1 were suggestive of additional amorphous random coil structures .…”

Section: Resultssupporting

confidence: 91%

“…Lopez‐Rubio et al reported increased integrity of starch based films on application of microfibrillar cellulose . Although starch has its intrinsic plasticizing properties on certain materials, sericin is known to form composites only after addition of cross‐linkers . It could be inferred from this observation that starch could partially exert its plasticizing effect on sericin.…”

Section: Resultsmentioning

confidence: 99%

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Development of Novel 2D Composites of Silk Sericin and Rice Starch and Application as Bio‐Compatible Scaffold for Cell Culturing

Dutta

Devi

2018

Starch Stärke

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Sericin and starch are waste materials of the silk and rice milling industries, respectively. Owing to their suitable physicochemical, mechanical and functional properties, the two materials are thermo‐mechanically conjugated and cast as films. Sericin forms a microfibrillar network structure which binds with gelatinized starch. Addition of crosslinkers, namely glycerol and polyethylene glycol results in smoother textured films, higher crystallinity, and thermal resistance as studied using XRD, FTIR, DSC, and TGA. Native crystalline patterns of both starch and sericin are lost during composite formation. Newer crystalline lamellae formation with enhanced crystallinity than the native materials is evident from XRD and DSC. Comparative analysis indicated polyethylene glycol to be a better cross linker than glycerol for the composite formation. Biocompatibility studies using L929 mice fibroblast cell lines demonstrated that the films supported normal growth and proliferation of cells without any toxic effect. This is well supported by fluorescence imaging and MTT assays. Enzymatic biodegradability using protease enzymes implied negligible weight loss of the films, strengthening their prospect to be manifested in cell culturing applications.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Development of Novel 2D Composites of Silk Sericin and Rice Starch and Application as Bio‐Compatible Scaffold for Cell Culturing

Dutta

Devi

2018

Starch Stärke

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“…Sericin is removed from the SF fibers by a degumming process, which is normally carried out under boiling alkaline conditions [ 53 ]. Researchers continuously work on improving the degumming process which typically requires reagents and organic solvents to obtain higher quality of pure SF.…”

Section: Sources Of Silk and Silk Fibroinmentioning

confidence: 99%

Silk Fibroin as a Functional Biomaterial for Tissue Engineering

Sun

Gregory

Tomeh

et al. 2021

IJMS

224

194

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Tissue engineering (TE) is the approach to combine cells with scaffold materials and appropriate growth factors to regenerate or replace damaged or degenerated tissue or organs. The scaffold material as a template for tissue formation plays the most important role in TE. Among scaffold materials, silk fibroin (SF), a natural protein with outstanding mechanical properties, biodegradability, biocompatibility, and bioresorbability has attracted significant attention for TE applications. SF is commonly dissolved into an aqueous solution and can be easily reconstructed into different material formats, including films, mats, hydrogels, and sponges via various fabrication techniques. These include spin coating, electrospinning, freeze drying, physical, and chemical crosslinking techniques. Furthermore, to facilitate fabrication of more complex SF-based scaffolds with high precision techniques including micro-patterning and bio-printing have recently been explored. This review introduces the physicochemical and mechanical properties of SF and looks into a range of SF-based scaffolds that have been recently developed. The typical TE applications of SF-based scaffolds including bone, cartilage, ligament, tendon, skin, wound healing, and tympanic membrane, will be highlighted and discussed, followed by future prospects and challenges needing to be addressed.

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“…Sericin, the “glue” on the surface of the silk fibers, has been proven to be one of the perfect substitutes for serum[ 26 ]. Sericin has the ability to promote the attachment and proliferation of mammalian cells[ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Novel sericin-based hepatocyte serum-free medium and sericin’s effect on hepatocyte transcriptome

Huang¹,

Peng²,

Li³

et al. 2018

WJG

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AIMTo develop a novel hepatocyte serum-free medium based on sericin, and to explore the effect of sericin on the hepatocyte transcriptome.METHODSA controlled trial comparing novel serum-free medium and other media: C3A cells were cultured in our novel serum-free medium, HepatoZYME, complete medium (DMEM/F12 with 100 mL/L FBS), and DMEM/F12, and then cell attachment, proliferation, and function as well as the biocompatibility of the media were assessed. A comparative study of serum-free media with or without 2 mg/mL sericin: the effect of sericin on C3A growth was assessed by cell viability and proliferation, the effect of sericin on C3A cell cycle distribution was determined by flow cytometry, and the effect of sericin on the C3A transcriptome was assessed by gene-chip array and RT-qPCR.RESULTSMore C3A cells attached to the plate containing our serum-free medium than to those containing HepatoZYME and DMEM/F12 at 24 h post-seeding. Both the viability and proliferation rate of C3A cells in sericin-based serum-free medium were superior to those of cells in HepatoZYME and DMEM/F12 (P < 0.001). The content of albumin and urea in our serum-free medium was significantly higher than that in HepatoZYME and DMEM/F12 throughout the whole culture period (P < 0.001) and was similar to that in complete medium at day 3, 4, and 5. In part 2, cell viability and proliferation were greater in the presence of 2 mg/mL sericin (P < 0.001), as was the proportion of cells in S phase (16.21% ± 0.98% vs 12.61% ± 0.90%, P < 0.01). Gene-chip array analysis indicated that the expression of CCR6, EGFR, and FOS were up-regulated by 2 mg/mL sericin, and RT-qPCR revealed that the expression of CCR6, EGFR, FOS, AKT1, JNK1, NFkB1, MMP-9, MEK2, ERK1/2 and MYC was up-regulated by 2 mg/mL sericin (P < 0.05).CONCLUSIONWe developed a novel hepatocyte serum-free medium. Sericin probably enhances cell attachment through the CCR6-Akt-JNK-NF-κB pathway and promotes cell proliferation through CCR6-mediated activation of the ERK1/2-MAPK pathway.

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Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture

Cited by 33 publications

References 49 publications

Development of Novel 2D Composites of Silk Sericin and Rice Starch and Application as Bio‐Compatible Scaffold for Cell Culturing

Development of Novel 2D Composites of Silk Sericin and Rice Starch and Application as Bio‐Compatible Scaffold for Cell Culturing

Silk Fibroin as a Functional Biomaterial for Tissue Engineering

Novel sericin-based hepatocyte serum-free medium and sericin’s effect on hepatocyte transcriptome

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