Non-micronized and micronized curcumin do not prevent the behavioral and neurochemical effects induced by acute stress in zebrafish

Sachett, Adrieli; Gallas‐Lopes, Matheus; Benvenutti, Radharani; Marcon, Matheus; Linazzi, Amanda Martins; Aguiar, Gean Pablo S.; Herrmann, Ana Paula; Oliveira, J. Vladimir; Siebel, Anna Maria; Piato, Ângelo

doi:10.1101/2021.10.11.463974

Cited by 2 publications

(1 citation statement)

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“…Micronization, a process that uses a supercritical fluid to change physicochemical aspects of substances, has been shown to improve the biological effects of substances such as N-acetylcysteine, resveratrol, and curcumin [5,[25][26][27][28]. In our previous studies, both curcumin and micronized curcumin (10 mg/kg, intraperitoneal) were unable to block the behavioral effects of acute restraint stress (90 minutes) [29] and unpredictable chronic stress (14 days) [28], while robust antioxidant effects were observed for micronized but not for its conventional preparation in adult zebrafish [28].…”

Section: Introductionmentioning

confidence: 97%

Micronized Curcumin Causes Hyperlocomotion in Zebrafish Larvae

et al. 2022

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Zebrafish larvae have been widely used in neuroscience and drug research and development. In the larval stage, zebrafish present a broad behavioral repertoire and physiological responses similar to adults. Curcumin (CUR), a major component of Curcuma longa L. (Zingiberaceae), has demonstrated the ability to modulate several neurobiological processes relevant to mental disorders in animal models. However, the low bioavailability of this compound can compromise its in vivo biological potential.Interestingly, it has been shown that micronization can increase the biological effects of several compounds. Thus, in this study, we compared the effects of acute exposure for 30 minutes to the following solutions: water (control), 0.1% DMSO (vehicle), 1 μM CUR, or 1 μM micronized curcumin (MC) in zebrafish larvae 7 days post-fertilization (dpf). We analyzed locomotor activity (open tank test), anxiety (light/dark test), and avoidance behavior (aversive stimulus test). Moreover, we evaluated parameters of oxidative status (thiobarbituric acid reactive substances and non-protein thiols levels).MC increased the total distance traveled and absolute turn angle in the open tank test.There were no significant differences in the other behavioral or neurochemical outcomes. The increase in locomotion induced by MC may be associated with a stimulant effect on the central nervous system, which was evidenced by the micronization process.

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Section: Introductionmentioning

confidence: 97%

Micronized Curcumin Causes Hyperlocomotion in Zebrafish Larvae

et al. 2022

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Micronized curcumin causes hyperlocomotion in zebrafish larvae

Sachett

Benvenutti

Reis

et al. 2021

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Zebrafish larvae have been widely used in neuroscience and drug research and development. In the larval stage, zebrafish present a broad behavioral repertoire and physiological responses similar to adults. Curcumin (CUR), a major component of Curcuma longa L. (Zingiberaceae), has demonstrated the ability to modulate several neurobiological processes relevant to mental disorders in animal models. However, the low bioavailability of this compound can compromise its in vivo biological potential. Interestingly, it has been shown that micronization can increase the biological effects of several compounds. Thus, in this study, we compared the effects of acute exposure for 30 minutes to the following solutions: water (control), 0.1% DMSO (vehicle), 1 μM CUR, or 1 μM micronized curcumin (MC) in zebrafish larvae 7 days post-fertilization (dpf). We analyzed locomotor activity (open tank test), anxiety (light/dark test), and avoidance behavior (aversive stimulus test). Moreover, we evaluated parameters of oxidative status (thiobarbituric acid reactive substances and non-protein thiols levels). MC increased the total distance traveled and absolute turn angle in the open tank test. There were no significant differences in the other behavioral or neurochemical outcomes. The increase in locomotion induced by MC may be associated with a stimulant effect on the central nervous system, which was evidenced by the micronization process.

show abstract

Non-micronized and micronized curcumin do not prevent the behavioral and neurochemical effects induced by acute stress in zebrafish

Cited by 2 publications

References 47 publications

Micronized Curcumin Causes Hyperlocomotion in Zebrafish Larvae

Micronized Curcumin Causes Hyperlocomotion in Zebrafish Larvae

Micronized curcumin causes hyperlocomotion in zebrafish larvae

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