Non-mammalian vertebrate embryos as models in nanomedicine

Giannaccini, Martina; Cuschieri, Alfred; Dente, Luciana; Raffa, Vittoria

doi:10.1016/j.nano.2013.09.010

Cited by 36 publications

(27 citation statements)

References 119 publications

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“…As nanotubes are being developed for numerous applications, understanding the implications of embryonic exposure to nanotubes is critical for developing safe nanotechnologies and toxicity analysis (Campagnolo et al 2012; Giannaccini et al 2014). Here, we observed a recovery pathway for developing Xenopus embryos to expel high aspect ratio nanotubes dispersed with membrane active Pluronic F127 that were injected 12.5 ± 7.5 μm below the embryo membrane at the one-to-two cell stage.…”

Section: Discussionmentioning

confidence: 99%

Developing Xenopus embryos recover by compacting and expelling single wall carbon nanotubes

Holt

Shawky

Dahl

et al. 2015

J of Applied Toxicology

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Single-wall carbon nanotubes are high aspect ratio nanomaterials that are being developed for use in materials, technological and biological applications due to their high mechanical stiffness, optical properties, and chemical inertness. Because of their prevalence, it is inevitable that biological systems will be exposed to nanotubes, yet studies of the effects of nanotubes on developing embryos have been inconclusive and are lacking for single-wall carbon nanotubes exposed to the widely studied model organism Xenopus laevis (African clawed frog). Microinjection of experimental substances into the Xenopus embryo is a standard technique for toxicology studies and cellular lineage tracing. Here we report the surprising finding that superficial (12.5 ± 7.5 μm below the membrane) microinjection of nanotubes dispersed with Pluronic F127 into one-to-two cell Xenopus embryos resulted in the formation and expulsion of compacted, nanotube-filled, punctate masses, at the blastula to mid-gastrula developmental stages, which we call “boluses”. Such expulsion of microinjected materials by Xenopus embryos has not been reported before and is dramatically different from the typical distribution of the materials throughout the progeny of the microinjected cells. Previous studies of microinjections of nanomaterials such as nanodiamonds, quantum dots or spherical nanoparticles report that nanomaterials often induce toxicity and remain localized within the embryos. In contrast, our results demonstrate an active recovery pathway for embryos after exposure to Pluronic F127-coated nanotubes, which we speculate is due to a combined effect of the membrane activity of the dispersing agent, Pluronic F127, and the large aspect ratio of nanotubes.

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Section: Discussionmentioning

confidence: 99%

Developing Xenopus embryos recover by compacting and expelling single wall carbon nanotubes

Holt

Shawky

Dahl

et al. 2015

J of Applied Toxicology

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“…For animal model study the zebra-fish (Danio rerio) was used, following recent works where non-mammalian vertebral animals are used as models in nanomedicine research [43]. Danio rerio were obtained from the facility established at the Department of Biology, University of Aveiro, Portugal.…”

Section: Zebra-fish Modelmentioning

confidence: 99%

Core–shell superparamagnetic nanoparticles with interesting properties as contrast agents for MRI

Carvalho

Domingues

Gonçalves

2015

Materials Chemistry and Physics

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h i g h l i g h t sCoreeshell nanoparticles with iron oxide cores and different coatings were obtained. The new nanosystems are efficient as negative contrast agent for MRI. The efficiency is discussed relating to the different coatings properties. Contrast enhancement is quantified in phantoms MRI and validated in an animal model. The potentiality of these new coreeshell NPs as negative CA for MRI is demonstrated and quantified. The longitudinal and transverse relaxivities of NPs with three different coating compositions were studied at a 7 T magnetic field: silica (TEOS), (3-aminopropyl) triethoxysilane (APTES) and (3-glycidoxypropyl) methyldiethoxysilane (GPTMS).Clearly, it was found that the coreeshell NPs efficiency as CA was strongly depend on the SPIONs coating.All the three coreeshell NPs studied presented a very small effect on the longitudinal relaxation time but a pronounced one on the transverse relaxation time, leading to a very high transverse longitudinal relaxivities ratio, decisive for their efficiency as negative CA for MRI.The effect of the coreeshell NPs on the MRI contrast enhancement is obtained and quantified in a set of MRI of agar phantoms obtained at 7 T magnetic field and with a imaging gradient field of 1.6 T/m. The coreeshell NPs were tested in Zebra-fish (Danio rerio) animal model. Zebra-fish MRI were obtained with animals injected with the three coreeshell NPs and the contrast enhancement validated.

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“…Zebrafish are prolific breeders, their embryos are transparent, develop rapidly, and offer multiple phenotypic and biochemical endpoints for assessing potential toxicity beyond general mortality (Bugel et al, 2014; Fako & Furgeson, 2009; Lin et al, 2013; Truong et al, 2011). In addition, the zebrafish genome is closely related to the human genome and many biological pathways are conserved between the species (Giannaccini et al, 2014). There have been numerous reports assessing the effects of CNTs on embryonic zebrafish development, but they often offer conflicting results and conclusions.…”

Section: Introductionmentioning

confidence: 99%

Toxicity assessment and bioaccumulation in zebrafish embryos exposed to carbon nanotubes suspended in Pluronic® F-108

et al. 2015

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Carbon nanotubes (CNTs) are often suspended in Pluronic® surfactants by sonication, which may confound toxicity studies because sonication of surfactants can create degradation products that are toxic to mammalian cells. Here, we present a toxicity assessment of Pluronic® F-108 with and without suspended CNTs using embryonic zebrafish as an in vivo model. Pluronic® sonolytic degradation products were toxic to zebrafish embryos just as they were to mammalian cells. When the toxic Pluronic® fragments were removed, there was little effect of pristine multi-walled CNTs (pMWNTs), carboxylated MWNTs (cMWNTs) or pristine single-walled carbon nanotubes (pSWNTs) on embryo viability and development, even at high concentrations. A gel electrophoretic method coupled with Raman imaging was developed to measure the bioaccumulation of CNTs by zebrafish embryos, and dose-dependent uptake of CNTs was observed. These data indicate that embryos accumulate pMWNTs, cMWNTs and pSWNTs yet there is very little embryo toxicity.

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Non-mammalian vertebrate embryos as models in nanomedicine

Cited by 36 publications

References 119 publications

Developing Xenopus embryos recover by compacting and expelling single wall carbon nanotubes

Developing Xenopus embryos recover by compacting and expelling single wall carbon nanotubes

Core–shell superparamagnetic nanoparticles with interesting properties as contrast agents for MRI

Toxicity assessment and bioaccumulation in zebrafish embryos exposed to carbon nanotubes suspended in Pluronic® F-108

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