Non-malignant B cells and chronic lymphocytic leukemia cells induce a pro-survival phenotype in CD14+ cells from peripheral blood

Abstract

References

Nurse like cells: chronic lymphocytic leukemia associated macrophages

“…After 14 days of co-culture, CD14 + adherent cells were first analyzed for their morphology. In case of samples from CLL patients or HD donors, we found large, round and adherent cells (figure 1a), as described by Bhattacharya and coll [9]. No difference in morphology between CLL-NLC and HD-NLC was shown.…”

“…In vitro, these cells were generated with a co culture of normal CD14 + cells with normal B lymphocytes and called healthy donors NLC (HD-NLC). Finally, these authors showed that HD-NLC can support the in vitro normal B lymphocytes survival [9] as CLL-NLC with CLL cells survival. However, in this study, the generation of HD-NLC and CLL-NLC was performed at a ratio not observed in the CLL patients, with one CD14 + cell for two B cells.…”

“…CLL-NLC are characterized by a high level of expression of CD68 [4] and CD163 [8], and by a gene expression pattern resembling that of Tumor Associated Macrophages (TAM) [8]. In 2011, two publications described cells derived from CD14 + cells of healthy donors (HD) expressing CD68, displaying a similar morphology [9], and a gene expression profile close to CLL-NLC [10]. In vitro, these cells were generated with a co culture of normal CD14 + cells with normal B lymphocytes and called healthy donors NLC (HD-NLC).…”

Inhibiting B-Cell Receptor Signaling Pathways in Chronic Lymphocytic Leukemia

“…In the lymphatic tissues, CLL cells interact with various neighbor cells, such as CD68 + monocyte-derived nurselike cells (NLCs) [6,7], actin/SMA + mesenchymal stromal cells (MSCs) [8], and CD4 + T cells [4,9]. These accessory cells provide growth signals and survival signals to the CLL cells that are largely contact-dependent (Fig.…”

Coming full circle: 70 years of chronic lymphocytic leukemia cell redistribution, from glucocorticoids to inhibitors of B-cell receptor signaling

“…3 For survival and expansion, CLL cells rely on external signals from the microenvironment and normally undergo spontaneous apoptosis in tissue culture unless they are cocultured with stromal cells. 2 In the lymphatic tissues, CLL cells interact with various stromal cells, such as CD68 ϩ nurselike cells (NLC), [4][5][6] smooth muscle actin-positive mesenchymal stromal cells, 7 and CD4 ϩ T cells. 8,9 By inference from in vitro studies, we assume that stromal cells provide growth and survival signals to the CLL cells that are largely contact-dependent and can cooperate with intrinsic oncogenic lesions.…”