Non-lithographic patterning of phage-displayed peptides with wrinkled elastomers

Abstract

The development of controlled patterning of phage (viruses) could expand opportunities for both fundamental studies and creating various materials platforms. Inducing the elastomeric instability of PDMS film provides a nonlithographic, tuneable, controlled method for generating micro/nanoscale wrinkle patterns. Phage display has emerged as a powerful method for selecting peptides that possess enhanced selectivity and binding affinity toward a variety of targets. In this report, we demonstrate the non-lithographic patterning of phage-displayed peptides with wrinkled elastomers. Our results show that the phage-displayed peptides can be patterned on specific locations in controlled and tuneable ways, be transferred to other substrates and induce the self-assembly of hybrid materials. We anticipate that these results could open up exciting opportunities in fundamental studies and in applications ranging from sensors, hybrid materials, self-assembly, surface and interface, to micro/nanoelectronics.

Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel

“…In this paper we demonstrate how to pattern PDMS and PAm hydrogel topographically via Parylene C as a bottom mask for PDMS and top mask for PAm hydrogel. PDMS wrinkled and anisotropic surface at nano and microscale was obtained using simple spin coating and avoiding complicated techniques actually proposed such as soft lithography 7 or elastomer pre-stretching 30 . Parylene C masks fabricated as described previously 6 were exploited for a new technique to pattern PAm hydrogel.…”

Identification of Functional Peptide Sequences to Lead the Design of Precision Polymers

“…The method provided easy access to patterned surfaces with different functionalities via a standard office laser printer and may contribute to the development of low‐cost diagnostic devices, enabling potential inexpensive point of care production. Swaminathan et al used phage display to select peptide sequences which show significant adsorption differences to effectively discriminate between silica glass and PDMS . These peptides offer a material selective coating system, where structures that originate from micro contact printing could be functionalized with fluorescence dyes or more complex bioactive labels.…”

A mild deposition of metallic materials on a plastic film enabled by phage display peptides

“…21 Recently, we also have identied phage displayed peptides for the iden-tication of graphene, 22 small molecule ink, 22 epoxy, 20 and PDMS polymer. 23,24 In this communication, we report for the rst time the identication of peptide binding motifs for PET via phage display technology and the mild deposition of metallic materials on PET with the designed bifunctional peptide. The selected phage displayed peptides are shown to bind to PET surfaces.…”

“…It has the PET-binding motif (DEYCCNN) screened from a phage display library and a silver (Ag) synthesis motif (NPSSLFRYLPSD). 24,25 A PET substrate (1 cm  1 cm) was incubated with the bifunctional peptides (10 ml, 50 mM) to immobilize the peptides on PET. The bifunctional peptide-immobilized PET was incubated with 30 mM silver nitrate for 3 days, which resulted in the growth of Ag nanoparticles on the PET surface.…”