Non‐lactose‐fermenting uropathogenic
            <i>Escherichia coli</i>
            from dogs: virulence profile characterization

Siqueira, Franciele Maboni; Carli, Sílvia De; Lopes, Cassiane Elisabete; Machado, Letícia Rocha; Vieira, Tatiana Regina; Pöppl, Álan Gomes; Cardoso, Marisa; Zaha, Arnaldo

doi:10.1111/lam.13454

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…( Nicoletti et al, 1988 ; Hossain, 2012 ; Chang et al, 2014 ; Yaratha et al, 2017 ; Johnson et al, 2019 ). Non-lactose fermenting (NLF) E. coli can be identified as colourless, transparent colonies on MacConkey agar and by negative lactose (sugar) fermentation tests ( Hossain, 2012 ; Siqueira et al, 2021 ). Various strains of E. coli are equipped to cause different forms of enteric and extraintestinal infections in human hosts with varying propensities ( Kaper et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Non-lactose fermenting Escherichia coli: Following in the footsteps of lactose fermenting E. coli high-risk clones

Mazumder

Hussain²,

Phelan³

et al. 2022

Front. Microbiol.

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Multi-resistant pathogenic strains of non-lactose fermenting Escherichia coli (NLF E. coli) are responsible for various intestinal and extraintestinal infections. Although several studies have characterised such strains using conventional methods, they have not been comprehensively studied at the genomic level. To address this gap, we used whole-genome sequencing (WGS) coupled with detailed microbiological and biochemical testing to investigate 17 NLF E. coli from a diagnostic centre (icddr,b) in Dhaka, Bangladesh. The prevalence of NLF E. coli was 10%, of which 47% (8/17) exhibited multi-drug resistant (MDR) phenotypes. All isolates (17/17) were confirmed as E. coli and could not ferment lactose sugar. WGS data analysis revealed international high-risk clonal lineages. The most prevalent sequence types (STs) were ST131 (23%), ST1193 (18%), ST12 (18%), ST501 (12%), ST167 (6%), ST73 (6%) and ST12 (6%). Phylogenetic analysis corroborated a striking clonal population amongst the studied NLF E. coli isolates. The predominant phylogroup detected was B2 (65%). The blaCTX-M-15 extended-spectrum beta-lactamase gene was present in 53% of isolates (9/17), whilst 64.7% (11/17) isolates were affiliated with pathogenic pathotypes. All extraintestinal pathogenic E. coli pathotypes demonstrated β-hemolysis. Our study underscores the presence of critical pathogens and MDR clones amongst non-lactose fermenting E. coli. We suggest that non-lactose fermenting E. coli be considered equally capable as lactose fermenting forms in causing intestinal and extraintestinal infections. Further, there is a need to undertake systematic, unbiased monitoring of predominant lineages amongst non-lactose fermenting E. coli that would help in better treatment and prevention strategies.

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Section: Introductionmentioning

confidence: 99%

Non-lactose fermenting Escherichia coli: Following in the footsteps of lactose fermenting E. coli high-risk clones

Mazumder

Hussain²,

Phelan³

et al. 2022

Front. Microbiol.

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Comparative genetic and pathogenic approaches of Escherichia coli isolated simultaneously from pyometra and urine of bitches

Lopes,

Weyh,

Moni

et al. 2024

Vet Res Commun

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Escherichia coli isolated from pyometra and cystitis in the same animal exhibit a wide phenotypic similarity

Moni,

Dias,

Lopes

et al. 2024

Journal of Applied Microbiology

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Aims Pyometra and cystitis caused by Escherichia coli are common diseases identified in canine or feline females. The origin of pyometra infection remains uncertain, and effective prevention strategies for this disease are still unknown. This study aimed to provide a phenotypic characterisation, including antimicrobial resistance and virulence profiles, of Endometrial Pathogenic (EnPEC) and Uropathogenic (UPEC) E. coli strains isolated simultaneously from the same animal. Methods and Results Sixteen E. coli strains, from eight different animals, were analysed in this study. The antimicrobial susceptibility profile of EnPEC and UPEC strains was determined using the disc diffusion method, which showed a similar susceptibility profile among strains (EnPEC and UPEC) from the same animal. The virulence profile of the strains was assessed through biofilm formation, as well as serum resistance abilities. EnPEC and UPEC strains from the same animal exhibited slight variations in their virulence and antimicrobial resistance capabilities. Overall, most of the strain pairs showed a high similarity in their ability to establish biofilms and survive in serum complement activity. Conclusions Overall, strains of E. coli isolated from both pyometra and cystitis in the same animal, despite presenting distinct clinical diseases, exhibit a wide phenotypic similarity, suggesting a common origin for the strains.

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Non‐lactose‐fermenting uropathogenic Escherichia coli from dogs: virulence profile characterization

Cited by 4 publications

References 29 publications

Non-lactose fermenting Escherichia coli: Following in the footsteps of lactose fermenting E. coli high-risk clones

Non-lactose fermenting Escherichia coli: Following in the footsteps of lactose fermenting E. coli high-risk clones

Comparative genetic and pathogenic approaches of Escherichia coli isolated simultaneously from pyometra and urine of bitches

Escherichia coli isolated from pyometra and cystitis in the same animal exhibit a wide phenotypic similarity

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