Non-Ionizing Label-Free Photoacoustic Imaging of Bones

Park, Eun-Yeong; Lee, Dong-Hyun; Lee, Chang-Ho; Kim, Chulhong

doi:10.1109/access.2020.3020559

Cited by 18 publications

(14 citation statements)

References 36 publications

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“…Those tissues considered “optical absorbers” such as blood and bone water are ideal for PA imaging where PA imaging can overcome limitations when using ultrasound alone (where the collected signal is reflected from the tissue layers and limits the achievable penetration depth). PA imaging can be acquired in situ , ex vivo, and in vivo ( Park et al, 2020 ) although in situ and ex vivo is often optimal due to competing signal with surrounding blood flow. The molecular vibrational state of water is affected based on whether it is bound or unbound in a tissue.…”

Section: Imaging and Spectral Methods To Quantify Bone Watermentioning

confidence: 99%

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

2022

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Water constitutes roughly a quarter of the cortical bone by volume yet can greatly influence mechanical properties and tissue quality. There is a growing appreciation for how water can dynamically change due to age, disease, and treatment. A key emerging area related to bone mechanical and tissue properties lies in differentiating the role of water in its four different compartments, including free/pore water, water loosely bound at the collagen/mineral interfaces, water tightly bound within collagen triple helices, and structural water within the mineral. This review summarizes our current knowledge of bone water across the four functional compartments and discusses how alterations in each compartment relate to mechanical changes. It provides an overview on the advent of- and improvements to- imaging and spectroscopic techniques able to probe nano-and molecular scales of bone water. These technical advances have led to an emerging understanding of how bone water changes in various conditions, of which aging, chronic kidney disease, diabetes, osteoporosis, and osteogenesis imperfecta are reviewed. Finally, it summarizes work focused on therapeutically targeting water to improve mechanical properties.

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Section: Imaging and Spectral Methods To Quantify Bone Watermentioning

confidence: 99%

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

2022

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“…With PAI, anatomical structures are easily visualized, and functional information such as blood oxygenation, oxygen saturation, blood flow, and metabolism can be obtained [ 18 , 19 ]. Owing to its flexible imaging system configuration, which allows a specific resolution and penetration depth, PAI is used in various biomedical applications [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. PA computed tomography (PACT) focuses on reconstructing an image from one-dimensional radial information defining the relationship between the PA source and the detector position.…”

Section: Introductionmentioning

confidence: 99%

In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy

Thao

Yoo

Park

et al. 2021

Sensors

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Vascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of PDT in mouse ear melanoma tumors. In particular, quantitative vasculature evaluation was conducted based on Hessian filter segmentation. Three-dimensional morphological PAM and depth-resolved images before and after PDT treatment were acquired. In addition, five quantitative vasculature parameters, including the PA signal, vessel diameter, vessel density, perfused vessel density, and vessel complexity, were analyzed to evaluate the influence of PDT on four different areas: Two melanoma tumors, and control and normal vessel areas. The quantitative and qualitative results successfully demonstrated the potential of the proposed PAM-based quantitative approach to evaluate the effectiveness of the PDT method.

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“…These benefits overcome the limited resolution of USI [ 18 , 19 , 20 ]. Moreover, PAM is a compact, inexpensive, non-ionized, label-free, functional, and real-time imaging modality [ 21 , 22 , 23 , 24 , 25 ]. It particularly aids the visualization of the distribution of intrinsic molecules in the body, such as hemoglobin, lipid, melanin, and proteins [ 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Pilot Study: Quantitative Photoacoustic Evaluation of Peripheral Vascular Dynamics Induced by Carfilzomib In Vivo

Thao

Chu

et al. 2021

Sensors

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Carfilzomib is mainly used to treat multiple myeloma. Several side effects have been reported in patients treated with carfilzomib, especially those associated with cardiovascular events, such as hypertension, congestive heart failure, and coronary artery disease. However, the side effects, especially the manifestation of cardiovascular events through capillaries, have not been fully investigated. Here, we performed a pilot experiment to monitor peripheral vascular dynamics in a mouse ear under the effects of carfilzomib using a quantitative photoacoustic vascular evaluation method. Before and after injecting the carfilzomib, bortezomib, and PBS solutions, we acquired high-resolution three-dimensional PAM data of the peripheral vasculature of the mouse ear during each experiment for 10 h. Then, the PAM maximum amplitude projection (MAP) images and five quantitative vascular parameters, i.e., photoacoustic (PA) signal, diameter, density, length fraction, and fractal dimension, were estimated. Quantitative results showed that carfilzomib induces a strong effect on the peripheral vascular system through a significant increase in all vascular parameters up to 50%, especially during the first 30 min after injection. Meanwhile, bortezomib and PBS do not have much impact on the peripheral vascular system. This pilot study verified PAM as a comprehensive method to investigate peripheral vasculature, along with the effects of carfilzomib. Therefore, we expect that PAM may be useful to predict cardiovascular events caused by carfilzomib.

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Non-Ionizing Label-Free Photoacoustic Imaging of Bones

Cited by 18 publications

References 36 publications

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy

Pilot Study: Quantitative Photoacoustic Evaluation of Peripheral Vascular Dynamics Induced by Carfilzomib In Vivo

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