Non-ionic surfactant-assisted controlled release of oxyresveratrol on dendritic fibrous silica for topical applications

Kerdmuanglek, Fonkaeo; Chomtong, Thitikorn; Boonsith, Suthida; Chutimasakul, Threeraphat; Iemsam-arng, Jayanant; Thepwatee, Sukanya

doi:10.1016/j.jcis.2023.05.050

Cited by 6 publications

(4 citation statements)

References 80 publications

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“…Eczema is one of the most common dermatological disorders that requires both immediate treatment and a long-term management strategy. ORV has long been reported as an antioxidant reagent with potential dermatological applications [ 2 , 30 ]. In addition, it has anti-proliferative effects on cancer cell lines as well as anti-inflammatory effects on macrophages and human microglial cells [ 4 , 6 , 8 , 9 , 10 ].…”

Section: Discussionmentioning

confidence: 99%

Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice

et al. 2023

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Oxyresveratrol (ORV) is one of the novel antioxidants having been extensively studied in recent years. One of the main sources of ORV is Artocarpus lakoocha, which has been used in traditional medicine in Thailand for decades. However, the role of ORV in skin inflammation has not been clearly demonstrated. Therefore, we investigated the anti-inflammatory effects of ORV on dermatitis model. The effect of ORV was examined on human immortalized and primary skin cells exposed to bacterial components including peptidoglycan (PGN) and lipopolysaccharide (LPS) and 2,4-Dinitrochlorobenzene (DNCB)-induced dermatitis mouse model. PGN and LPS were used to induce inflammation on immortalized keratinocytes (HaCaT) and human epidermal keratinocytes (HEKa). We then performed MTT assay, Annexin V and PI assay, cell cycle analysis, real-time PCR, ELISA and Western blot in these in vitro models. H&E staining, immunohistochemistry (IHC) staining with CD3, CD4 and CD8 markers were used to evaluate the effects of ORV in in vivo model of skin inflammation using BALB/c mice. Pretreatment of HaCaT and HEKa cells with ORV inhibited pro-inflammatory cytokine production through inhibition of NF-κB pathway. In DNCB-induced dermatitis mouse model, ORV treatment reduced lesion severity, and skin thickness and numbers of CD3, CD4 and CD8 T cells in the sensitized skin of mice. In conclusion, it has been demonstrated that ORV treatment can ameliorate inflammation in the in vitro models of skin inflammation and in vivo models of dermatitis, suggesting a therapeutic potential of ORV for treatment of skin diseases particularly eczema.

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Section: Discussionmentioning

confidence: 99%

Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice

et al. 2023

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“…This conclusion was supported by both TG and DTA results, as shown in figures 4 and 5. The TG curve for DFNS exhibited a weight loss of approximately 10% only in the region below 100 °C, indicating a typical water evaporation process [42,50]. The TG curve for DFNS@KM showed a weight loss of around 75%, indicating a high loading amount and drug degradation due to the higher thermal stability of inorganic silica carriers than organic guest molecules.…”

Section: Characterization Of Dfns@kmmentioning

confidence: 98%

“…These nanoparticles exhibited a specific surface area of 692 m 2 g −1 and a pore volume of 1.12 cm 3 g −1 . They also employed the ethanol evaporation technique to co-load oxyresveratrol with a non-ionic surfactant onto KCC-1, achieving a drug release efficiency of over 80% [42]. In our study, we chose to utilize 1-pentanol as a replacement for cyclohexane as the oil solvent due to its higher water solubility and increased stability.…”

Section: Introductionmentioning

confidence: 99%

A study on the pesticides-loading capacity of dendritic fibrous nano silica synthesized from 1-pentanol-water microemulsion with a low oil-water ratio

Liu,

Chen

et al. 2023

Nanotechnology

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Dendritic fibrous nano silica (DFNS) represents an optimal carrier material for pesticide constituents, due to its radial accessibility channels and high specific surface area. A low-energy methodology for synthesizing DFNS at a low volume ratio of oil to water is provided by employing 1-pentanol as the oil solvent in the microemulsion synthesis system, renowned for its remarkable stability and exceptional solubility. The DFNS@KM nano-pesticide was fabricated using a diffusion supported loading (DiSupLo) method and kresoxim-methyl (KM) as the template drug. Findings from FT-IR, XRD, TG, DTA, and BET analyses revealed the physical adsorption of KM onto the synthesized DFNS without any chemical bonding, with KM mainly existing in an amorphous state within the channels. High-performance liquid chromatography (HPLC) measurements demonstrated that the loading amount and encapsulation efficiency of DFNS@KM were primarily dependent on the KM to DFNS ratio, with minimal effects observed from loading temperature and time. The loading amount and encapsulation efficiency of DFNS@KM were found to be 63.09% and 84.12%, respectively. Furthermore, DFNS effectively prolonged the release of KM, with a cumulative release rate of 85.43% over 180 hours. The successful loading of pesticide components into DFNS synthesized with a low oil-to-water ratio provides theoretical support for the industrialization of nano-pesticides, with significant implications for enhancing pesticide utilization, reducing pesticide dosage, augmenting agricultural efficiency, and promoting sustainable agricultural development.

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“…In addition, eczema is one of the most common dermatological disorders that requires both immediate treatment and a long-term management strategy. ORV has long been reported as an antioxidant reagent with potential dermatological applications (93,94). In addition, it has anti-proliferative effects on various cancer cell lines (HepG2, MCF-7, HT-19, HSC-3, HN-8 and HN-30) as well as anti-inflammatory effects on macrophages (RAW264.7) and human microglial cells (HMC3) (8,9,76,85,86).…”

Section: Discussionmentioning

confidence: 99%

Effect of Oxyresveratrol on Inflammatory Skin Diseases||

Tran

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Background:�Oxyresveratrol (ORV) is one of the novel antioxidants having been extensively studied in recent years. One of the main sources of ORV is�Artocarpus lakoocha, which has been used in traditional medicine in Thailand for decades. However, the role of ORV in skin inflammation has not been clearly demonstrated. Therefore, we investigated the anti-inflammatory effects of ORV on dermatitis model. Objectives:�To determine the effect and mechanism of ORV on anti-proliferation, anti-inflammation and anti-Staphyloccocus aureus�with keratinocytes and��to clarify the treating efficacy of ORV on skin inflammatory mouse model. Materials and methods:�The effect of ORV was examined on human immortalized and primary skin cells exposed to bacterial components including peptidoglycan (PGN) and lipopolysaccharide (LPS). PGN and LPS were used to induce inflammation on immortalized keratinocytes (HaCaT) and human epidermal keratinocytes (HEKa). We then performed MTT assay, Annexin V and PI assay, cell cycle analysis, real-time PCR, ELISA and Western blot in these�in vitro�models. On mouse model, 2,4-Dinitrochlorobenzene�(DNCB)�was used to induce dermatitis. H&E staining, immunohistochemistry (IHC) staining with CD3, CD4 and CD8 markers were used to evaluate the effects of ORV in�in vivo�model of skin inflammation using BALB/c mice. Disc diffusion method on�Staphyloccocus aureus�was used to test the anti-bacterial effect of ORV.� Results:�Treatment with ORV on HaCaT cells showed the anti-proliferation effect through inducing apoptosis by activating caspase-3. Pretreatment of HaCaT and HEKa cells with ORV inhibited pro-inflammatory cytokine production through inhibition of NF-?B pathway. In DNCB-induced dermatitis mouse model, ORV treatment reduced severity of skin lesion, and skin thickness and numbers of CD3, CD4 and CD8 T cells in the sensitized skin of mice. The combination of ORV and several antibiotics at the dosage using on keratinocytes showed the synergestic effect on�anti-Staphyloccocus aureus�through the increase of inhibiting zone.� Conclusion:�ORV treatment can ameliorate inflammation in the in vitro models of skin inflammation and in vivo models of dermatitis, suggesting a therapeutic potential of ORV for treatment of skin diseases particularly eczema. ORV can facilitate the anti-bacterial property of antibiotics on�Staphylococcus aureus.�

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Non-ionic surfactant-assisted controlled release of oxyresveratrol on dendritic fibrous silica for topical applications

Cited by 6 publications

References 80 publications

Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice

Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice

A study on the pesticides-loading capacity of dendritic fibrous nano silica synthesized from 1-pentanol-water microemulsion with a low oil-water ratio

Effect of Oxyresveratrol on Inflammatory Skin Diseases||

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