Non-invasive stem cell tracking in hindlimb ischemia animal model using bio-orthogonal copper-free click chemistry

Lee, Si Yeon; Lee, Sang‐Min; Lee, Jangwook; Yhee, Ji Young; Yoon, Hwa In; Park, Soon‐Jung; Koo, Heebeom; Moon, Sung; Lee, Hyukjin; Cho, Yong Woo; Kang, Sung Soo; Lee, Sang Yup; Kim, Kwangmeyung

doi:10.1016/j.bbrc.2016.09.132

Cited by 28 publications

(24 citation statements)

References 19 publications

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“…Several studies have reported that the labeling mechanism typically involves the adherence and diffusion of a lipophilic cyanine-based dye across the phospholipid cell membrane bilayer. Other modes include transporters, endocytosis, and phagocytosis (2,3).…”

Section: Introductionmentioning

confidence: 99%

Effect of Cell Labeling on the Function of Human Pluripotent Stem Cell-Derived Cardiomyocytes

Choi

Cho

Kim

et al. 2020

IJSC

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Cell labeling technologies are required to monitor the fate of transplanted cells in vivo and to select target cells for the observation of certain changes in vitro. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been transplanted for the treatment of heart injuries or used in vitro for preclinical cardiac safety assessments. Cardiomyocyte (CM) labeling has been used in these processes to facilitate target cell monitoring. However, the functional effect of the labeling agent on hiPSC-CMs has not been studied. Therefore, we investigated the effects of labeling agents on CM cellular functions. 3'-Dioctadecyloxacarbocyanine perchlorate (DiO), quantum dots (QDs), and a DNA plasmid expressing EGFP using Lipo2K were used to label hiPSC-CMs. We conclude that the hiPSC-CM labeling with DiO and QDs does not induce arrhythmogenic effects but rather improves the mRNA expression of cardiac ion channels and Ca 2＋ influx by L-type Ca 2＋ channels. Thus, DiO and QD labeling agents may be useful tools to monitor transplanted CMs, and further in vivo influences of the labeling agents should be investigated in the future.

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Section: Introductionmentioning

confidence: 99%

Effect of Cell Labeling on the Function of Human Pluripotent Stem Cell-Derived Cardiomyocytes

Choi

Cho

Kim

et al. 2020

IJSC

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“…Transplanted chondrocytes could be detected for 4 weeks using in vivo imaging analysis, and this labeling was found to have a minimal effect on the cartilage formation of chondrocytes. Lee et al also demonstrated that metabolic glycoengineering and the SPAAC reaction was useful for tracking transplanted stem cells [31]. Since MSCs are able to home to inflammation sites and tumor tissues in response to chemokines released from inflammation sites or tumors [46,65,66], the migration of MSCs was traced.…”

Section: Click Chemistry As a Tool For Cell Engineering In Cell Trmentioning

confidence: 99%

“…Azide groups on cell surfaces after metabolic glycoengineering gradually disappear due to the hydrolysis of glycans by neuraminidase in cells after internalization [25,30]. However, it has been reported that azide groups were detected on the cell surface for at least 14 days [31], suggesting that metabolic glycoengineering is a suitable tool for cell labeling or functionalization through click chemistry.…”

Section: Introductionmentioning

confidence: 99%

Click Chemistry as a Tool for Cell Engineering and Drug Delivery

2019

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Click chemistry has great potential for use in binding between nucleic acids, lipids, proteins, and other molecules, and has been used in many research fields because of its beneficial characteristics, including high yield, high specificity, and simplicity. The recent development of copper-free and less cytotoxic click chemistry reactions has allowed for the application of click chemistry to the field of medicine. Moreover, metabolic glycoengineering allows for the direct modification of living cells with substrates for click chemistry either in vitro or in vivo. As such, click chemistry has become a powerful tool for cell transplantation and drug delivery. In this review, we describe some applications of click chemistry for cell engineering in cell transplantation and for drug delivery in the diagnosis and treatment of diseases.

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“…It does not require genetic modifications. So far, this approach has only been used for near-Infrared fluorescence (NIR) with stem cells and tumor cells (52,53). Although NIR imaging is non-toxic and cheap, its limited spatial resolution and poor penetration through tissue complicate its use in clinical imaging.…”

Section: Metabolically Engineered Cells and Click Chemistrymentioning

confidence: 99%

Cell Tracking in Cancer Immunotherapy

et al. 2020

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The impressive development of cancer immunotherapy in the last few years originates from a more precise understanding of control mechanisms in the immune system leading to the discovery of new targets and new therapeutic tools. Since different stages of disease progression elicit different local and systemic inflammatory responses, the ability to longitudinally interrogate the migration and expansion of immune cells throughout the whole body will greatly facilitate disease characterization and guide selection of appropriate treatment regiments. While using radiolabeled white blood cells to detect inflammatory lesions has been a classical nuclear medicine technique for years, new non-invasive methods for monitoring the distribution and migration of biologically active cells in living organisms have emerged. They are designed to improve detection sensitivity and allow for a better preservation of cell activity and integrity. These methods include the monitoring of therapeutic cells but also of all cells related to a specific disease or therapeutic approach. Labeling of therapeutic cells for imaging may be performed in vitro, with some limitations on sensitivity and duration of observation. Alternatively, in vivo cell tracking may be performed by genetically engineering cells or mice so that may be revealed through imaging. In addition, SPECT or PET imaging based on monoclonal antibodies has been used to detect tumors in the human body for years. They may be used to detect and quantify the presence of specific cells within cancer lesions. These methods have been the object of several recent reviews that have concentrated on technical aspects, stressing the differences between direct and indirect labeling. They are briefly described here by distinguishing ex vivo (labeling cells with paramagnetic, radioactive, or fluorescent tracers) and in vivo (in vivo capture of injected radioactive, fluorescent or luminescent tracers, or by using labeled antibodies, ligands, or pre-targeted clickable substrates) imaging methods. This review focuses on cell tracking in specific therapeutic applications, namely cell therapy, and particularly CAR (Chimeric Antigen Receptor) T-cell therapy, which is a fast-growing research field with various therapeutic indications. The potential impact of imaging on the progress of these new therapeutic modalities is discussed.

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Non-invasive stem cell tracking in hindlimb ischemia animal model using bio-orthogonal copper-free click chemistry

Cited by 28 publications

References 19 publications

Effect of Cell Labeling on the Function of Human Pluripotent Stem Cell-Derived Cardiomyocytes

Effect of Cell Labeling on the Function of Human Pluripotent Stem Cell-Derived Cardiomyocytes

Click Chemistry as a Tool for Cell Engineering and Drug Delivery

Cell Tracking in Cancer Immunotherapy

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