Non-invasive pulsed electric field food processing: Proof-of-principle experiments

Novac, B.M.; Banakhr, Fahd; Smith, I.R.; Pécastaing, Laurent; Ruscassie, R.; Ferron, A. De; Pignolet, P.; Liu, Yi

doi:10.1109/ipmhvc.2012.6518797

Cited by 3 publications

(2 citation statements)

References 3 publications

(5 reference statements)

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“…Recent research focuses on using noninvasive electrodes, where direct contact of liquid with electrodes will be prohibited so that the major drawback of PEF treatment i.e., electrolysis will be prevented. Such efforts include adhering high permittivity ceramic cylinders with the electrodes, metal coating on electrodes etc [7,8]. This paper focuses on application of high electric field through electrodes which are immersed in water for PEF applications.…”

Section: Introductionmentioning

confidence: 99%

Electric field Analysis of Water Electrodes for Noninvasive Pulsed Electric field Applications

Ramaswamy¹,

Prabu²,

Gowrisree³

2019

IJITEE

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Electroporation is an effective phenomenon of inactivating viable pathogens present in the liquid food for pulsed electric field (PEF) applications. It is a technique which depends on applied electric field strength for causing pores on cell plasma membrane. The various parameters which affect the electroporation efficacy are, the electric field intensity, pulse width, number of pulses, pulse interval and the electrode. The electrode provides a contact between the high voltage pulse generator and the liquid food, and it plays an important role in getting the required inactivation outcome. The electric field distribution varies based on electrode designs. Parallel plate electrodes are generally used due to the uniform electric field it delivers in the inactivation area, where high possibility of microbial inactivation will occur. This paper analyses the effectiveness of round edged parallel plate electrodes immersed in water which provides uniform electric field distribution in the inactivation area. Analyses have been performed on electric field distribution through four kinds of materials such as glass, alumina, quartz and plexiglass, which contains these electrodes in the center filled with sterile water. The electrodes are circular, and edge smoothened and hence the field distribution is also analyzed on electrode edges. The distance between the electrodes including the surface material is kept at 5 mm. The diameter of the electrodes are 40 mm and the electric field simulations are implemented in ANSYS MAXWELL v 15.0. Based on results it is reported that alumina required less peak voltage for generating 20 kV/cm field strength (nominal field required for bacterial inactivation) when compared with other materials. Also alumina exhibited less reduction of field travelling through it, and resulted in 82% of field application in the inactivation area which is comparatively higher than other materials. The results indicate that alumina is highly recommended for future noninvasive pulsed electric field applications.

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Section: Introductionmentioning

confidence: 99%

Electric field Analysis of Water Electrodes for Noninvasive Pulsed Electric field Applications

Ramaswamy¹,

Prabu²,

Gowrisree³

2019

IJITEE

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“…A conceptual PEF test chamber with dielectric electrodes is discussed in [9] and [10]. In this test cell, the metal electrodes are covered by a dielectric layer.…”

Section: Introductionmentioning

confidence: 99%

Pulsed electric field assisted treatment of microorganisms for lysis

Qin

Timoshkin

Wilson

et al. 2013

2013 19th IEEE Pulsed Power Conference (PPC)

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Pulsed electric field (PEF) treatment can be used to facilitate microbial cell lysis. The present work is to investigate the effect of PEF treatment on micro-algae. One traditional PEF test cell with parallel-plane metallic electrodes and one conceptual test cell with parallel-plane dielectric electrodes are designed and built. Both test cells and the PEF system are modeled using a lump element circuit approach and the results of simulations performed using PSpice software are presented. PEF treatment of microalgae is conducted using both test cells. The efficiency of the PEF treatment is assessed by measurement of optical transmission of microalgae in growth media at different time intervals (growth curve). Also, microalgae were visual inspected using optical microscope before and after PEF treatment. It was shown that smaller number of high voltage impulses is required to achieve efficient inactivation of microalgae in the test cell with metallic electrodes as compared with the conceptual test cell with the ceramic dielectric barriers.

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Capacitive coupling increases the accuracy of cell-specific tumour disruption by electric fields

Wimberger

Köhler

Ehmoser

et al. 2020

Bioelectrochemistry

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Irreversible electroporation holds great potential for cell-specific lysis due to the size-dependent susceptibility of cells to externally imposed electric fields. Previous attempts at selective cell lysis lead to significant overlap between affected populations and struggle with inconsistent biological outcome. We propose that charge transfer at the electrode-liquid interface is responsible by inducing multifactorial effects originating from both the electric field and electrochemical reactions. A promising remedy is the coating of electrodes with a high-k dielectric layer. The resulting capacitive coupling restores the selective potential of electric field mediated lysis in a microfluidic setup. Initial experiments show the consistent depletion of erythrocytes from whole blood while leaving leukocytes intact. The same is true for the reproducible and selective depletion of Jurkat and MCF-7 cells in a mixture with leukocytes. Unexpectedly, the observed order of lysis cannot be correlated with cell size. This implies that the cellular response to capacitive coupling features a selective characteristic that is different from conventional lysis configurations..

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Non-invasive pulsed electric field food processing: Proof-of-principle experiments

Cited by 3 publications

References 3 publications

Electric field Analysis of Water Electrodes for Noninvasive Pulsed Electric field Applications

Electric field Analysis of Water Electrodes for Noninvasive Pulsed Electric field Applications

Pulsed electric field assisted treatment of microorganisms for lysis

Capacitive coupling increases the accuracy of cell-specific tumour disruption by electric fields

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