Non-invasive primate head restraint using thermoplastic masks

Drucker, Caroline B.; Carlson, Monica L.; Toda, Kohji; DeWind, Nicholas K.; Platt, Michael L.

doi:10.1016/j.jneumeth.2015.06.013

Cited by 28 publications

(15 citation statements)

References 37 publications

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“…A three-dimensional printed angular adaptor was also used to adjust the penetration angle to maximize the collinearity of the saccade vectors obtained by stimulation (see below “Neurophysiological recordings”). Head restraint was realized by fitting a thermoplastic mask individually for each animal 59 .…”

Section: Methodsmentioning

confidence: 99%

Sensorimotor transformation elicits systematic patterns of activity along the dorsoventral extent of the superior colliculus in the macaque monkey

2019

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The superior colliculus (SC) is an excellent substrate to study sensorimotor transformations. To date, the spatial and temporal properties of population activity along its dorsoventral axis have been inferred from single electrode studies. Here, we recorded SC population activity in non-human primates using a linear multi-contact array during delayed saccade tasks. We show that during the visual epoch, information appeared first in dorsal layers and systematically later in ventral layers. During the delay period, the laminar organization of low-spiking rate activity matched that of the visual epoch. During the pre-saccadic epoch, spiking activity emerged first in a more ventral layer, ~ 100 ms before saccade onset. This buildup of activity appeared later on nearby neurons situated both dorsally and ventrally, culminating in a synchronous burst across the dorsoventral axis, ~ 28 ms before saccade onset. Collectively, these results reveal a principled spatiotemporal organization of SC population activity underlying sensorimotor transformation for the control of gaze.

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Section: Methodsmentioning

confidence: 99%

Sensorimotor transformation elicits systematic patterns of activity along the dorsoventral extent of the superior colliculus in the macaque monkey

2019

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“…Drucke et al 27 used thermoplastic masks as a noninvasive mold to restrain a primate head and to enable a wide range of neuroscientific experiments. They indicated that these masks allowed for the acquisition of clearer MRI images.…”

Section: Discussionmentioning

confidence: 99%

A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments

Rencai

Hou

Wang

et al. 2021

Sci Rep

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This study aimed to develop and validate a novel rabbit fixator made from a thermoplastic mask for awake imaging experiments. When heated in a hot-water bath at 65–70 °C for 2–5 min, the thermoplastic mask became soft and could be molded to fit over the entire body of an anesthetized rabbit (4 ml of 3% pentobarbital sodium solution by intramuscular injection). Twenty rabbits were randomly divided into fixator (n = 10) and anesthesia (n = 10) groups. The animals’ vital signs, stress hormones (cortisol and adrenaline), and subjective image quality scores for the computed tomography (CT), positron emission tomography (PET), and magnetic resonance imaging (MRI) scanning were measured and compared. Phantom CT, MRI and PET studies were performed to assess the performance with and without the thermoplastic mask by using image agents at different concentrations or with different radioactivity. The respiration rate (RR), systolic blood pressure (SBP), diastolic blood pressure (DBP), peripheral capillary oxygen saturation (SpO2) and body temperature (T) decreased after anesthesia (all P < 0.05) but did not significantly decrease after fixation (all P > 0.05). The heart rate (HR), cortisol and adrenaline did not significantly decrease after either anesthesia or fixation (all P > 0.05). The subjective image quality scores for the CT and MRI images of the head, thorax, liver, kidney, intestines and pelvis and the subjective image quality scores for the PET images did not significantly differ between the two groups (all P > 0.05). For all examined organs except the muscle, 18F-FDG metabolism was lower after fixation than after anesthesia, and was almost identical of liver between two groups. The phantom study showed that the CT values, standard uptake values and MR T2 signal values did not differ significantly with or without the mask (all P > 0.05). A novel rabbit fixator created using a thermoplastic mask could be used to obtain high-quality images for different imaging modalities in an awake and near-physiological state.

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“…A 3-D printed angular adaptor was also used to adjust the penetration angle to maximize the collinearity of the saccade vectors obtained by stimulation (see below Neurophysiological recordings ). Head restraint was realized by fitting a thermoplastic mask individually for each animal 74 .…”

Section: Methodsmentioning

confidence: 99%

Time-course of population activity along the dorsoventral extent of the superior colliculus during delayed saccade tasks

Massot

Jagadisan

Gandhi

2019

Preprint

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20The superior colliculus (SC) is an excellent substrate to study functional organization of 21 sensorimotor transformations. We used linear multi-contact array recordings to analyze the spatial and 22 temporal properties of population activity along the SC dorsoventral axis during delayed saccade tasks. 23During the visual epoch, information appeared first in dorsal layers and systematically later in ventral layers. 24In the ensuing delay period, the laminar organization of low-spiking rate activity matched that of the visual 25 epoch. During the pre-saccadic epoch, spiking activity emerged first in a more ventral layer, ~100ms before 26 saccade onset. This buildup of activity appeared later on nearby neurons situated both dorsally and 27 ventrally, culminating in a synchronous burst across the dorsoventral axis, ~28ms before saccade onset. 28 Stimulation of individual contacts on the laminar probe produced saccades of similar vectors. Collectively, 29 the results reveal a principled spatiotemporal organization of SC population activity underlying sensorimotor 30 transformation for the control of gaze. 31 32 Introduction 33Our interactions with the environment are mediated via brain networks that transform sensory 34 signals to motor actions at the appropriate time. In the context of gaze control, this sensorimotor 35 transformation entails processing of incoming visual information and generating a movement command to 36 appropriately redirect the line of sight. The superior colliculus (SC) in the midbrain modulates its activity in 37 response to both stimulus presentation and movement generation, as well as during the interval between 38 the two events. Like cortex, the SC is composed of distinct layers. Its superficial layers are predominantly 39 driven by visual processing structures like the retina and primary visual cortex, while its deeper layers 40 communicate a broad spectrum of information with many cortical and noncortical areas [1][2][3] . It also has a 41 canonical organization with established microcircuits for communication both within and across layers 4 . 42Finally, it has a topographic representation of visual space and for generation of gaze shifts to those 43 locations 5 . Thus, the SC is ideally suited to study the neural correlates of sensorimotor transformation. 44Despite a wealth of knowledge about the anatomical organization of the SC and the functional 45 properties of individual SC neurons, current understanding about the link between structural and functional 46 organization at the population level is limited. For instance, it is unclear whether the properties of individual 47 neurons exhibit systematic spatiotemporal organization during the sensorimotor transformation, and how 48 such organization is linked to the microarchitecture of the SC network. Bridging structure with function helps 49 to not only understand the computations underlying the transformation but also to build biologically-inspired 50 network models of sensorimotor learning and behavior 6,7 . 51Linear microelectrodes...

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Non-invasive primate head restraint using thermoplastic masks

Cited by 28 publications

References 37 publications

Sensorimotor transformation elicits systematic patterns of activity along the dorsoventral extent of the superior colliculus in the macaque monkey

Sensorimotor transformation elicits systematic patterns of activity along the dorsoventral extent of the superior colliculus in the macaque monkey

A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments

Time-course of population activity along the dorsoventral extent of the superior colliculus during delayed saccade tasks

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