Non-invasive photoacoustic screening of blood vasculature during anti-angiogenesis using CAM assay

Khan, Suhel; Pathak, Priyanka; Vasudevan, Srivathsan; Nayak, Debasis

doi:10.1364/osac.432084

Cited by 7 publications

(7 citation statements)

References 34 publications

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“…The effect of the low-frequency region (0 to 1 MHz) and high-frequency region (1 to 10 MHz) at the spectral energy was investigated. The spectral energy at low-frequency region EL and high-frequency region EH was normalized with the Es, then resultant relative energies ELS and EHS were used to monitor the quantitative change at a specific frequency region, which can provide crucial information for tissue characterization 6 , 22 …”

Section: Methodsmentioning

confidence: 99%

“…This theoretical understanding was both confirmed through an experimental investigation involving black rubber and validated via simulation studies, as depicted in Refs. 22 and 23 . These simulation studies revealed that an increase in density leads to a corresponding rise in sound speed, thereby causing a shift in the frequency spectra toward the higher frequency range.…”

Section: Methodsmentioning

confidence: 99%

“…The spectral energy at low-frequency region

and high-frequency region

was normalized with the

, then resultant relative energies

and

were used to monitor the quantitative change at a specific frequency region, which can provide crucial information for tissue characterization. 6 , 22 …”

Section: Methodsmentioning

confidence: 99%

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Development of a cost-effective compact diode-laser-based photoacoustic sensing instrument for breast tissue diagnosis

Khan,

Mukhopadhyay,

Vasudevan

et al. 2024

J. Biomed. Opt.

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The photoacoustic (PA) technique, a noninvasive pump-probe technique, has found interesting applications in biomedical tissue diagnosis over the last decade. To take it a step further to clinical applications, the PA technique needs to be designed as an instrument focusing on a compact design, reducing the cost, and quickly providing a quantitative diagnosis.Aim: This work presents a design and characterization of a cost-effective, compact PA sensing instrument for biomedical tissue diagnosis.Approach: A compact laser diode case design is developed to house several laser diodes for PA excitation, and a pulsed current supply unit is also developed in-house to power the laser diodes to generate a 25 ns current pulse at a frequency of 20 kHz. After PA experimental data acquisition, the signal's frequency spectra were calculated to characterize the tissue quantitatively and correlated with their mechanobiological properties. Results:The corresponding dominant frequency peak in the PA spectral response (PASR) study was low in the fibrofatty normal breast tissue 0.26 AE 0.03 MHz, compared to the dominant frequency peak of 1.60 AE 0.016 MHz in the fibrocystic disease tissue, which had increased glandular and stromal elements, thereby increased tissue density. The histopathological findings correlated with the PASR results, and the fibrocystic breast disease tissue exhibited a higher dominant frequency peak and energy compared to the normal breast tissue. Conclusions:We experimented with an in vitro PASR study of fibrocystic human breast tissues and successfully differentiated different tissue types using quantitative spectral parameters peak frequency, mean frequency, and spectral energy. This gives the potential to take this technique further for cost-effective and quick clinical applications.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Development of a cost-effective compact diode-laser-based photoacoustic sensing instrument for breast tissue diagnosis

Khan,

Mukhopadhyay,

Vasudevan

et al. 2024

J. Biomed. Opt.

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“…The window is closed and retaped, the eggs were placed back into the incubator to allow the vasculature and growth for 5 to 7 days. After the incubation period the reduction in the growth of the veins were recorded and the inhibition of angiogenesis measured (12).…”

Section: Anti-angiogenic Activity Of Synthetic Ampmentioning

confidence: 99%

Characterization and biochemical activities of novel functional antimicrobial peptide (AMP) from Trichogramma chilonis

Sunil¹,

Kumar²,

Pramod³

et al. 2022

Biomedicine

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Introduction and Aim: The antimicrobial peptides (AMPs) are generally found in invertebrates, mammals, birds, plants and insects. AMPs produced by insect parasitoids contribute to innate immunity to resist infection due to lack of adaptive immunity. T. chilonis is one of the most effective endoparasitoid wasps for controlling lepidopterous insects. Several attempts have been made to isolate, characterize and develop a commercially viable product of AMPs from various insect sources. The present study aimed to characterize AMP from T. chilonis for potential antimicrobial and anti-cancer properties. Methods: AMP was identified through T. chilonis transcriptome sequence and designed in silico and synthesized. Its purity was quantified using RP-HPLC, and the mass identified by mass spectrophotometry. LC/MS-MS was employed to predict the sequence and the BLAST program used to compare the sequence. AMP was tested for haemolytic activity and antimicrobial activity. Two pathogenic bacteria and fungal strains were used and IC50 values and MIC values were predicted against microbial strains. Results: Synthetic peptide was found to be 95% homogenous with molecular weight of 3.48 kD. The peptide was identified to be a novel antimicrobial peptide consisting of 33 amino acid residues, and has a low computed instability index of -0.1.55 with high hydrophobic ratio of 27.27%. The antimicrobial activity revealed that T. chilonis antimicrobial peptide (TC-AMP) strongly inhibits the growth of selected human bacterial and fungal pathogens. While the haemolytic assay showed that the peptide did not obliterate human RBC in vitro. TC-AMP also showed an efficient inhibition of angiogenesis by in vivo model as evident by inhibition of vascularization. Conclusions: AMP derived from the parasitoid has a potent antibiotic and anti-angiogenesis property. The peptide can be used as a potential antimicrobial and anticancer drug in near future with more detailed studies on its targeted applications.

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“…[22][23][24] Yet, it is crucial to note that spectral parameters contain valuable mechano-biological information that can be extracted. By harnessing this information, the technique can not only serve as a screening tool but also provide vital insights from a diagnostic perspective [24][25][26] in a costeffective manner. For instance, the relaxation time of the PA time-domain signal is associated with the elasticity of the sample.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic Spectral Response using Ultrasound and Interferometric Sensors: A Correlation Study for a High Bandwidth Real-Time Blood Vasculature Monitoring Application in a Chick-Embryo Chorioallantoic Membrane (CAM) Model

Khan,

Nayak,

Vasudevan

2023

Appl Spectrosc

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Photoacoustic (PA) spectral response technique has shown good promise in efficient preclinical tissue diagnosis by depicting mechano-biological properties due to high spatial resolution and penetration depth. The conventional PA-based system is a pump–probe technique that utilizes neodymium-doped yttrium aluminum garnet pulsed laser as a pump and an ultrasound sensor as a probe. For biomedical studies, high-speed PA signals need to be acquired, requiring higher bandwidth ultrasound sensors. While the bandwidth increases, they exhibit a very low signal-to-noise ratio that inhibits acquiring PA signals of biomedical samples. An interferometer-based probe has recently been investigated as a potential ultrasound probe for obtaining PA signals as an alternative. This optical PA detection technique offers high sensitivity by combining low acoustic impedance with high electromechanical coupling. However, there is a lack of exploration of the same for real-time biomedical studies. This work shows the development of a homodyne Mach-Zehnder interferometer-based PA spectral response (PASR) followed by a correlation study between the conventional ultrasound sensor and the interferometer-based sensor. Further, this study demonstrates the capability of continuous monitoring of vascular growth and the effect of an antidrug (Cisplatin) on the vasculature tested on a chick-embryo chorioallantoic membrane model. PASR was able to monitor growth changes within one day, which was not possible with conventional methods. This opens up potential possibilities for using this technique in biomedical applications.

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Non-invasive photoacoustic screening of blood vasculature during anti-angiogenesis using CAM assay

Cited by 7 publications

References 34 publications

Development of a cost-effective compact diode-laser-based photoacoustic sensing instrument for breast tissue diagnosis

Development of a cost-effective compact diode-laser-based photoacoustic sensing instrument for breast tissue diagnosis

Characterization and biochemical activities of novel functional antimicrobial peptide (AMP) from Trichogramma chilonis

Photoacoustic Spectral Response using Ultrasound and Interferometric Sensors: A Correlation Study for a High Bandwidth Real-Time Blood Vasculature Monitoring Application in a Chick-Embryo Chorioallantoic Membrane (CAM) Model

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