Non‐invasive online detection of nitric oxide from plants and some other organisms by mass spectrometry

Abstract

SummaryAs nitric oxide (NO) is a key messenger in many organisms, reliable techniques for the detection of NO are essential. Here, it is shown that a combination of membrane inlet mass spectrometry (MIMS) and restriction capillary inlet mass spectrometry (RIMS) allows for the fast, speci®c, and non-invasive online detection of NO that has been emitted from tissue cultures of diverse organisms, or from whole plants. As an advantage over other NO assays, MIMS/RIMS discriminates nitrogen isotopes and simultaneously measures NO and O 2 (and other gases) from the same sample. MIMS/RIMS technology may thus help to identify the source of gaseous NO in cells, and elucidate the relationship between primary gas metabolism and NO formation. Using RIMS, it is demonstrated that the novel fungicide F 500 j triggers NO production in plants.

Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars

“…NO (approximately 1 nmol) and other gas detection from gaseous and liquid phases (Conrath et al, 2004). Another MS approach, where gaseous .…”

“…NO was passed through a polyethylene membrane into the vacuum of a mass spectrometer. A similar technique was employed by Conrath et al (2004) in the combined membrane inlet mass spectrometry (MIMS)/restriction capillary mass spectrometry (RIMS) approach, where . NO can either diffuse from a culture through a membrane (MIMS) or from the gas phase through a thin restriction capillary (RIMS) directly into a benchtop mass spectrometer (Conrath et al, 2004).…”

“…) could be accurately measured, which were equivalent to thresholds for chemiluminescent detection (Archer, 1993) and greater than a 10-fold increased sensitivity compared to the RIMS approach (Conrath et al, 2004). Measurements of .…”

Impact of Pyraclostrobin (F-500) on Crop Plants

“…Growth stimulation in plants with the strobilurin fungicides has been related to a reduction in the incidence of disease as well as increased nitrate uptake and assimilation in small grains (Köehle et al, 2003). Research has shown that pyraclostrobin was important in stimulating nitric oxide, a key messenger in plants (Conrath, Amoroso, Köhle, & Sultemeyer, 2004).…”

Effects of Pyraclostrobin on Growth and Yield of Curly Red Chili (Capsicum Annum L.)

“…So, the application of pyraclostrobin affected net assimilasion rate. As reported by Conrath et al (2004), several researches shown that pyraclostrobin stimulated an increase in nitrate uptake and assimilation.…”