Non-invasive nuclear magnetic resonance analysis of male and female embryo metabolites during in vitro embryo culture

Rubessa, M.; Ambrosi, Andrea; González-Peña, Dianelys; Polkoff, Kathryn M.; Wheeler, Matthew B.

doi:10.1007/s11306-018-1414-0

Cited by 13 publications

(16 citation statements)

References 39 publications

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“…cannot be used in ART procedures. Initial studies on oocytes, spermatozoa and embryos using NMR spectroscopy focused on using 31 P NMR to understand energy metabolism and related physiological conditions like intracellular pH, motility and growth [80][81][82][83]. The application of NMR spectroscopy in assisted reproduction on a variety of experimental samples collected during various stages of ART procedures is schematically represented in figure 3.…”

Section: Nmr Spectroscopy In Assisted Reproductionmentioning

confidence: 99%

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

et al. 2020

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Infertility affects approximately 15–20% of individuals of reproductive age worldwide. Over the last 40 years, assisted reproductive technology (ART) has helped millions of childless couples. However, ART is limited by a low success rate and risk of multiple gestations. Devising methods for selecting the best gamete or embryo that increases the ART success rate and prevention of multiple gestation has become one of the key goals in ART today. Special emphasis has been placed on the development of non-invasive approaches, which do not require perturbing the embryonic cells, as the current morphology-based embryo selection approach has shortcomings in predicting the implantation potential of embryos. An observed association between embryo metabolism and viability has prompted researchers to develop metabolomics-based biomarkers. Nuclear magnetic resonance (NMR) spectroscopy provides a non-invasive approach for the metabolic profiling of tissues, gametes and embryos, with the key advantage of having a minimal sample preparation procedure. Using NMR spectroscopy, biologically important molecules can be identified and quantified in intact cells, extracts or secretomes. This, in turn, helps to map out the active metabolic pathways in a system. The present review covers the contribution of NMR spectroscopy in assisted reproduction at various stages of the process.

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Section: Nmr Spectroscopy In Assisted Reproductionmentioning

confidence: 99%

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

et al. 2020

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“…More recently, Rubessa et al 49 conducted a study on embryonic metabolism using bovine culture medium samples throughout the days of the cycle by using 1 H NMR spectroscopy. They divided the study into two parts: in the first one they found that, in general, on D + 3 pre‐embryos that developed correctly experienced increased consumption of some of the amino acids referred to above (alanine, leucine, isoleucine, valine, histidine, tyrosine, lysine, methionine, and phenylalanine), the concentration of lactate increased, and concentrations of myoinositol and citrate decreased on D + 1 to D + 3.…”

Section: Resultsmentioning

confidence: 99%

Solution NMR in human embryo culture media as an option for assessment of embryo implantation potential

et al. 2021

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NMR offers the potential to holistically screen hundreds of metabolites and has already proved to be a powerful technique able to provide a global picture of metabolic changes in a wide range of biological systems underlying complex and multifactorial matrixes. This review covers the literature until May 2020 centered on the early prediction of the viability of in vitro developed embryos using several analytical techniques, including NMR. Nowadays, the predominant non‐invasive technique for selecting viable embryos is based on morphology, where variables associated with the rate of cleavage and blastocyst formation are evaluated by the embryologist following standardized criteria that are somewhat subjective. This morphological approach is therefore inadequate for the prediction of embryo quality, and several studies have focused on developing new non‐invasive methods using molecular approaches based particularly on metabolomics. This review outlines the potential of NMR as one of these non‐invasive in vitro methods based on the analysis of spent embryo culture media.

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“…Metabolic behaviour between male and female bovine embryos differs during the in vitro development. For example, amino acid consumption differs between male and female embryos 44 .…”

Section: Discussionmentioning

confidence: 99%

Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Idrissi

Bourhis

Lefèvre

et al. 2021

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Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with lipid accumulation in the cytoplasm mainly due to in vitro culture conditions. The objective of this study was to evaluate the lipid composition of biopsied and sexed embryos, produced either in vivo or in vitro from the same Holstein heifers before and after a slow freezing protocol. Lipid extracts were analysed by liquid chromatography-high resolution mass spectrometry, which enabled the detection of 496 features. Our results highlighted a lipid enrichment of IVP embryos in triglycerides and oxidised glycerophospholipids and a reduced abundance in glycerophospholipids. The slow freezing process affected the lipid profiles of IVP and IVD embryos similarly. Lysophosphatidylcholine content was reduced when embryos were frozen/thawed. In conclusion, the embryonic lipid profile is impacted by IVP and slow freezing protocols but not by sex. Lysophosphatidylcholine seemed highly sensitive to cryopreservation and might contribute to explain the lower quality of frozen embryos. Further studies are required to improve embryo freezability by modulating the lipidome.

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Non-invasive nuclear magnetic resonance analysis of male and female embryo metabolites during in vitro embryo culture

Cited by 13 publications

References 39 publications

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

Solution NMR in human embryo culture media as an option for assessment of embryo implantation potential

Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

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