Non-invasive multispectral optoacoustic tomography resolves intrahepatic lipids in patients with hepatic steatosis

Fasoula, Nikolina‐Alexia; Karlas, Angelos; Prokopchuk, Olga; Katsouli, Nikoletta; Bariotakis, Michael; Liapis, Evangelos; Goetz, Anna; Kallmayer, Michael; Reber, Josefine; Novotny, Alexander; Friess, Helmut; Ringelhan, Marc; Schmid, Roland M.; Eckstein, Hans‐Henning; Hofmann, Susanna M.; Ntziachristos, Vasilis

doi:10.1016/j.pacs.2023.100454

Cited by 10 publications

(9 citation statements)

References 41 publications

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“…If the spectral window with major hemoglobin absorption can be avoided, secondary chromophores—such as melanin, lipid, and collagen—composing a biological tissue can also be selectively targeted. For instance, there is rich absorption by lipid in the spectral region around 930 nm, and this spectral characteristic was exploited by Fasoula et al to measure lipid accumulation in the livers of patients with hepatic steatosis [ 173 ]. Their investigation began with a pilot study involving 5 participants with steatosis and 5 without, and used a clinical hand-held MSOT/US system.…”

Section: Advances In Functional Photoacoustic Imaging At Different Sc...mentioning

confidence: 99%

“…Comparative normalized ratio spectra, and normalized liver ROI/background mean pixel intensity ratio at 930 nm for c for the whole participant cohort (steatotic, n = 5; healthy, n = 5), and d for the whole animal model cohort (high fat diet, n = 5; regular chow diet, n = 5). The images are reproduced with permission from [ 173 ] …”

Section: Advances In Functional Photoacoustic Imaging At Different Sc...mentioning

confidence: 99%

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Functional photoacoustic imaging: from nano- and micro- to macro-scale

2023

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Functional photoacoustic imaging is a promising biological imaging technique that offers such unique benefits as scalable resolution and imaging depth, as well as the ability to provide functional information. At nanoscale, photoacoustic imaging has provided super-resolution images of the surface light absorption characteristics of materials and of single organelles in cells. At the microscopic and macroscopic scales. photoacoustic imaging techniques have precisely measured and quantified various physiological parameters, such as oxygen saturation, vessel morphology, blood flow, and the metabolic rate of oxygen, in both human and animal subjects. This comprehensive review provides an overview of functional photoacoustic imaging across multiple scales, from nano to macro, and highlights recent advances in technology developments and applications. Finally, the review surveys the future prospects of functional photoacoustic imaging in the biomedical field.

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Section: Advances In Functional Photoacoustic Imaging At Different Sc...mentioning

confidence: 99%

Section: Advances In Functional Photoacoustic Imaging At Different Sc...mentioning

confidence: 99%

Functional photoacoustic imaging: from nano- and micro- to macro-scale

2023

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“…Moreover, the integration of photoacoustic (PA) imaging with clinical US imaging is actively progressing in clinical studies. 11 – 20 This integration enhances the ability to capture subtle pathological signs and improves diagnostic accuracy. 21 – 23 As a hybrid optical and acoustical imaging modality, PA imaging provides unique photochemical contrast capabilities at previously uncharted depths within biological tissue, 24 – 29 surpassing the capabilities of other optical imaging methods.…”

Section: Introductionmentioning

confidence: 99%

Video-rate endocavity photoacoustic/harmonic ultrasound imaging with miniaturized light delivery

Oh,

Kim,

Sung

et al. 2024

J. Biomed. Opt.

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. Significance Endocavity ultrasound (US) imaging is a frequently employed diagnostic technique in gynecology and urology for the assessment of male and female genital diseases that present challenges for conventional transabdominal imaging. The integration of photoacoustic (PA) imaging with clinical US imaging has displayed promising outcomes in clinical research. Nonetheless, its application has been constrained due to size limitations, restricting it to spatially confined locations such as vaginal or rectal canals. Aim This study presents the development of a video-rate (20 Hz) endocavity PA/harmonic US imaging (EPAUSI) system. Approach The approach incorporates a commercially available endocavity US probe with a miniaturized laser delivery unit, comprised of a single large-core fiber and a line beamshaping engineered diffuser. The system facilitates real-time image display and subsequent processing, including angular energy density correction and spectral unmixing, in offline mode. Results The spatial resolutions of the concurrently acquired PA and harmonic US images were measured at and in the radial direction, respectively, and 1.22 deg and 1.50 deg in the angular direction, respectively. Furthermore, the system demonstrated its capability in multispectral PA imaging by successfully distinguishing two clinical dyes in a tissue-mimicking phantom. Its rapid temporal resolution enabled the capture of kinetic dye perfusion into an ex vivo porcine ovary through the depth of porcine uterine tissue. EPAUSI proved its clinical viability by detecting pulsating hemodynamics in the male rat’s prostate in vivo and accurately classifying human blood vessels into arteries and veins based on measurements. Conclusions Our proposed EPAUSI system holds the potential to unveil previously overlooked indicators of vascular alterations in genital cancers or endometriosis, addressing pressing requirements in the fields of gynecology and urology.

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“…Contrary to purely optical techniques, MSOT employs fast light pulses to produce and sense ultrasound waves and is therefore unaffected by light scattering. MSOT, especially after recent image quality improvements [21] , can be employed at depths of up to 3–4 cm and has already been used in the study of tumors and cardiovascular, metabolic, endocrine, neuromuscular and inflammatory diseases [20] , [22] , [23] , [24] , [25] , [26] , [27] , [28] . Particularly relevant for the current study, MSOT has shown great potential for imaging skeletal muscle under disturbed blood flow conditions (arterial and venous occlusion) [29] , [30] , as well as before and after cycling exercise [31] .…”

Section: Introductionmentioning

confidence: 99%