Non-invasive monitoring of tissue scaffold degradation using ultrasound elasticity imaging

Kim, Kang; Jeong, Claire G.; Hollister, Scott J.

doi:10.1016/j.actbio.2008.02.010

Cited by 118 publications

(98 citation statements)

References 46 publications

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“…Further, imaging could make the processes of tissue replacement and regeneration more effective and less invasive than conventional implants. Increased imaging characterization of both natural and engineered tissues would lead to improved design of tissue engineering therapies (9). It is widely recognized that twodimensional cell culture systems are artificial and that cells raised in these systems are phenotypically different from those grown in a three-dimensional environment.…”

Section: Imaging Of Engineered Tissues: Opportunitiesmentioning

confidence: 99%

Bioengineering and Imaging Research Opportunities Workshop V: Summary of Findings on Imaging and Characterizing Structure and Function in Native and Engineered Tissues

et al. 2008

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and 2006) (1-4) was to identify and characterize opportunities for scientific research and engineering development in biomedical engineering and imaging.Given the topic of BIROW V, "Imaging and Characterizing Structure and Function in Native and Engineered Tissues," the meeting focused on four areas of scientific research that offer opportunities for major developments in biomedical engineering and imaging. These four areas were (a) heterogeneous single-cell measurements and their integration into tissue and organism models; (b) functional, molecular, and structural imaging of engineered tissue in vitro and in vivo; (c) new technologies for characterizing cells and tissues in situ; and (d) imaging for targeted cell, gene, and drug delivery.Each area was addressed in a plenary session, where several speakers presented their analysis of the subject and the research opportunities and challenges it presents. Speaker presentations were then followed by audience discussion. Each area was then the focus of discussion at one of four concurrent breakout sessions. Each breakout session provided a forum for discussion of research opportunities from the perspective of the objectives of the Roadmap program of the National Institutes of Health (5). These objectives are as follows: (a) Does it deepen understanding of fundamental biology? (b) Does it promote collaboration of multidisciplinary teams? (c) Does it reshape clinical research and promote discovery? and (d) Does it improve people's health?

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Section: Imaging Of Engineered Tissues: Opportunitiesmentioning

confidence: 99%

Bioengineering and Imaging Research Opportunities Workshop V: Summary of Findings on Imaging and Characterizing Structure and Function in Native and Engineered Tissues

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Further, imaging could make the processes of tissue replacement and regeneration more effective and less invasive compared with conventional implants. Increased imaging characterization of both natural and engineered tissues would lead to improved design of tissue engineering therapies (9).…”

Section: Imaging Of Engineered Tissues: Opportunitiesmentioning

confidence: 99%

Bioengineering and Imaging Research Opportunities Workshop V: A summary on Imaging and Characterizing Structure and Function in Native and Engineered Tissues

et al. 2008

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The Fifth Bioengineering and Imaging Research Opportunities Workshop (BIROW V) was held on January [18][19] 2008. As with previous BIROW meetings, the purpose of BIROW V was to identify and characterize research and engineering opportunities in biomedical engineering and imaging. The topic of this BIROW meeting was Imaging and Characterizing Structure and Function in Native and Engineered Tissues. Under this topic, four areas were explored in depth: 1) Heterogeneous single-cell measurements and their integration into tissue and organism models; 2) Functional, molecular, and structural imaging of engineered tissue in vitro and in vivo; 3) New technologies for characterizing cells and tissues in situ; 4) Imaging for targeted cell, gene, and drug delivery. The topic of BIROW V was Imaging and Characterizing Structure and Function in Native and Engineered Tissues. The meeting focused on four areas of scientific research that offer opportunities for major developments in biomedical engineering and imaging. The four areas are as follows:1. Heterogeneous single-cell measurements and their integration into tissue and organism models. 2. Functional, molecular, and structural imaging of engineered tissue in vitro and in vivo.

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“…A variety of biomedical imaging strategies such as fluorescence microscopy, magnetic resonance imaging, and ultrasound (US) imaging, in particular for tissue engineering applications, have been investigated. [1][2][3] These imaging modalities have been used to provide noninvasive, real-time evaluation of scaffold stability, 4 biomacromolecule accumulation, 5 and cellular activities. 2,6,7 Among a number of biomedical imaging modalities, US imaging has been widely utilized in the clinic as well as the biomedical research field due to its simplicity and noninvasive operation.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of gold nanotracers to track adipose-derived stem cells in a PEGylated fibrin gel for dermal tissue engineering applications

Chung¹,

Nam²,

Ricles³

et al. 2013

IJN

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Evaluating the regenerative capacity of a tissue-engineered device in a noninvasive and synchronous manner is critical to determining the mechanisms for success in clinical applications. In particular, directly tracking implanted cells in a three-dimensional (3D) scaffold is desirable in that it enables the monitoring of cellular activity in a specific and localized manner. The authors' group has previously demonstrated that the PEGylation of fibrin results in a 3D scaffold that supports morphologic and phenotypic changes in mesenchymal stem cells that may be advantageous in wound healing applications. Recently, the authors have evaluated adipose-derived stem cells (ASCs) as a mesenchymal cell source to regenerate skin and blood vessels due to their potential for proliferation, differentiation, and production of growth factors. However, tracking and monitoring ASCs in a 3D scaffold, such as a PEGylated fibrin gel, have not yet been fully investigated. In the current paper, nanoscale gold spheres (20 nm) as cell tracers for ASCs cultured in a PEGylated fibrin gel were evaluated. An advanced dual-imaging modality combining ultrasound and photoacoustic imaging was utilized to monitor rat ASCs over time. The ASCs took up gold nanotracers and could be detected up to day 16 with high sensitivity using photoacoustic imaging. There were no detrimental effects on ASC morphology, network formation, proliferation, and protein expression/secretion (ie, smooth muscle α-actin, vascular endothelial growth factor, matrix metalloproteinase-2, and matrix metalloproteinase-9) associated with gold nanotracers. Therefore, utilization of gold nanotracers can be an effective strategy to monitor the regenerative process of a stem cell source in a 3D gel for vascular and dermal tissue engineering applications.

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Non-invasive monitoring of tissue scaffold degradation using ultrasound elasticity imaging

Cited by 118 publications

References 46 publications

Bioengineering and Imaging Research Opportunities Workshop V: Summary of Findings on Imaging and Characterizing Structure and Function in Native and Engineered Tissues

Bioengineering and Imaging Research Opportunities Workshop V: Summary of Findings on Imaging and Characterizing Structure and Function in Native and Engineered Tissues

Bioengineering and Imaging Research Opportunities Workshop V: A summary on Imaging and Characterizing Structure and Function in Native and Engineered Tissues

Evaluation of gold nanotracers to track adipose-derived stem cells in a PEGylated fibrin gel for dermal tissue engineering applications

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