Non-invasive molecular imaging and reporter genes

Serganova, Inna; Мороз, Е. А.; Moroz, Maxim A.; Pillarsetty, Nagavarakishore; Blasberg, Ronald G.

doi:10.2478/s11535-006-0007-5

Cited by 5 publications

(5 citation statements)

References 145 publications

(154 reference statements)

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“…However, once we solve the abovementioned problems, the development and approval of a multimodality reporter gene-reporter probe system for human studies will progress. This reporter gene system can be applied in many different fields, and will play an important role in exploring human physiology and pathology 217 .…”

Section: Clinical Applications Of Reporter Gene Imagingmentioning

confidence: 99%

Multimodality reporter gene imaging: Construction strategies and application

Li¹,

Wang²,

Liu³

et al. 2018

Theranostics

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Molecular imaging has played an important role in the noninvasive exploration of multiple biological processes. Reporter gene imaging is a key part of molecular imaging. By combining with a reporter probe, a reporter protein can induce the accumulation of specific signals that are detectable by an imaging device to provide indirect information of reporter gene expression in living subjects. There are many types of reporter genes and each corresponding imaging technique has its own advantages and drawbacks. Fused reporter genes or single reporter genes with products detectable by multiple imaging modalities can compensate for the disadvantages and potentiate the advantages of each modality. Reporter gene multimodality imaging could be applied to trace implanted cells, monitor gene therapy, assess endogenous molecular events, screen drugs, etc. Although several types of multimodality imaging apparatus and multimodality reporter genes are available, more sophisticated detectors and multimodality reporter gene systems are needed.

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Section: Clinical Applications Of Reporter Gene Imagingmentioning

confidence: 99%

Multimodality reporter gene imaging: Construction strategies and application

Li¹,

Wang²,

Liu³

et al. 2018

Theranostics

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“…Thus, the targeting of firefly luciferase to the C. albicans cell surface might also provide a means of circumventing this limitation. However, firefly luciferase uses ATP for the conversion of luciferin into light (37), and therefore the efficient monitoring of firefly luciferase activity in vivo might be limited by the availability of ATP in extracellular fluids. Thus, future experiments should be aimed at improving the mode of administration of coelenterazine and its stability in vivo in order to take advantage of the exciting properties of gLUC59 for the real-time monitoring of C. albicans infections in live animals.…”

Section: Vol 77 2009 Luciferase Reporter For Imaging C Albicans Inmentioning

confidence: 99%

“…Pioneering work by Contag et al (6) demonstrated that bioluminescent Salmonella could be localized to specific tissues in live animals, allowing the temporal monitoring of the infection process and of the efficacy of antimicrobial treatment. This approach has now been extended to numerous pathogenic bacteria, virus, and parasites (19), and several luciferases are available for in vivo imaging, including firefly luciferase (fLUC from Photinus pyralis), which catalyzes light production from luciferin and ATP, and sea pansy luciferase (rLUC) and Gaussia princeps luciferase (gLUC), which catalyze light production from coelenterazine in an ATP-independent manner (37,44,47). Recently, Doyle et al (8,9) showed that light emitted by C. albicans strains expressing the firefly luciferase gene under the control of the strong C. albicans ENO1 promoter could be detected in animals with induced vulvovaginal candidiasis that had been subjected to a vaginal lavage with a solution containing luciferin.…”

mentioning

confidence: 99%

A Multifunctional, Synthetic Gaussia princeps Luciferase Reporter for Live Imaging of Candida albicans Infections

et al. 2009

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Real-time monitoring of the spatial and temporal progression of infection/gene expression in animals will contribute greatly to our understanding of host-pathogen interactions while reducing the number of animals required to generate statistically significant data sets. Sensitive in vivo imaging technologies can detect low levels of light emitted from luciferase reporters in vivo, but the existing reporters are not optimal for fungal infections. Therefore, our aim was to develop a novel reporter system for imaging Candida albicans infections that overcomes the limitations of current luciferase reporters for this major fungal pathogen. This luciferase reporter was constructed by fusing a synthetic, codon-optimized version of the Gaussia princeps luciferase gene to C. albicans PGA59, which encodes a glycosylphosphatidylinositol-linked cell wall protein. Luciferase expressed from this PGA59-gLUC fusion (referred to as gLUC59) was localized at the C. albicans cell surface, allowing the detection of luciferase in intact cells. The analysis of fusions to strong (ACT1 and EFT3), oxidative stress-induced (TRX1, TRR1, and IPF9996), and morphogenesis-dependent (HWP1) promoters confirmed that gLUC59 is a convenient and sensitive reporter for studies of gene regulation in yeast or hyphal cells, as well as a flexible screening tool. Moreover, the ACT1-gLUC59 fusion represented a powerful tool for the imaging of disease progression in superficial and subcutaneous C. albicans infections. gLUC59 and related cell surfaceexposed luciferase reporters might find wide applications in molecular biology, cell biology, pathobiology, and high-throughput screens.Candida albicans is responsible for a large fraction of fungal infections in humans (5) and, as such, has received considerable attention from the research community over the last two decades. C. albicans now represents an invaluable model for dissecting the interplay between fungal pathogens and their hosts at the molecular level (31,32,43,45,50). Studies of host-pathogen interactions have been greatly facilitated by the use of ex vivo infection models where isolated microorganisms and host cells or reconstituted tissues are brought into contact and the kinetics of pathogen and host cell responses are monitored (12,14,23,36,45). Yet, animal models remain necessary complements to ex vivo infection models, because none of these models fully reflect the development of clinical infections. Animal models allow researchers to monitor the behavior of mutant microorganisms or the expression of reporter genes in the complex environments of organs and in the presence of a fully functional or debilitated immune system (3,20,24).A current limitation of animal models is the need to sacrifice animals in order to image microorganisms at the site of infection. In particular, studies aimed at evaluating whether conditions known to trigger the expression of a specific C. albicans gene in vitro are encountered at sites of infection have often relied on the detection of a reporter in tissue section...

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“…Based on the three technologies, there have been numerous instrumentations. For instance, nuclear imaging includes positron emission tomography (PET) [15][16][17] and single photon emission computed tomography (SPECT) [18], while optical imaging mainly involves fluorescence molecular tomography (FMT) [14,19] and bioluminescent imaging (BLI) [20][21][22]. The difference between FMT and BLI can be found in [23].…”

Section: Introductionmentioning

confidence: 99%