Non-invasive measurement of glucose uptake of skeletal muscle tissue models using a glucose nanobiosensor

Obregón, Raquel; Ahadian, Samad; Ramón‐Azcón, Javier; Chen, Luyang; Fujita, Takeshi; Shiku, Hitoshi; Chen, Mingwei; Matsue, Tomokazu

doi:10.1016/j.bios.2013.06.020

Cited by 20 publications

(20 citation statements)

References 37 publications

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“…Myocardial ischemia injury results in dysfunctional contraction of the left ventricle, which can be exacerbated by reperfusion. Myosin heavy chain (e.g., Myh7b, Myh6) and α-actinin (Actc1) proteins are closely related to cardiac contractile and sarcomeric function [35]. Our results detected that some causative genes, such as Myh7b, Myl3, Tnni3, Actc1, were up-regulated in I/R hearts and were reversed by EA pretreatment.…”

Section: Resultsmentioning

confidence: 74%

Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion

Huang

Shen

et al. 2014

Molecules

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This study investigated genome-wide gene expressions and the cardioprotective effects of electro-acupuncture pretreatment at the PC6 Neiguan acupoint on myocardial ischemia reperfusion (I/R) injury. Male SD rats were randomly divided into four groups: sham operation (SO), I/R, electro-acupuncture at the PC6 Neiguan acupoint pretreatment (EA) and electro-acupuncture at non-acupoint pretreatment (NA). Compared with the I/R group, the survival rate of the EA group was significantly increased, the arrhythmia score, infarction area, serum concentrations of CK, LDH and CK-Mb and plasma level of cTnT were significantly decreased. RNA-seq results showed that 725 genes were up-regulated and 861 genes were down-regulated under I/R conditions compared to the SO group; both EA and NA reversed some of these gene expression levels (592 in EA and 238 in NA group). KEGG pathway analysis indicated that these genes were involved in multiple OPEN ACCESSMolecules 2014, 19 16159 pathways, including ECM, MAPK signaling, apoptosis, cytokine and leukocyte pathways. In addition, some pathways were uniquely regulated by EA, but not NA pretreatment, such as oxidative stress, cardiac muscle contraction, gap junction, vascular smooth muscle contraction, hypertrophic, NOD-like receptor, and P53 and B-cell receptor pathways. This study was first to reveal the gene expression signatures of acute myocardial I/R injury and electro-acupuncture pretreatment in rats.

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Section: Resultsmentioning

confidence: 74%

Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion

Huang

Shen

et al. 2014

Molecules

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“…C2C12 myotubes were immunostained as detailed elsewhere4748. In brief, the C2C12 myotubes were fixed with 3–4% paraformaldehyde for 12 min, followed by a wash with PBS.…”

Section: Methodsmentioning

confidence: 99%

Hybrid hydrogels containing vertically aligned carbon nanotubes with anisotropic electrical conductivity for muscle myofiber fabrication

Ahadian

Ramón‐Azcón

Estili

et al. 2014

Sci Rep

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222

178

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Biological scaffolds with tunable electrical and mechanical properties are of great interest in many different fields, such as regenerative medicine, biorobotics, and biosensing. In this study, dielectrophoresis (DEP) was used to vertically align carbon nanotubes (CNTs) within methacrylated gelatin (GelMA) hydrogels in a robust, simple, and rapid manner. GelMA-aligned CNT hydrogels showed anisotropic electrical conductivity and superior mechanical properties compared with pristine GelMA hydrogels and GelMA hydrogels containing randomly distributed CNTs. Skeletal muscle cells grown on vertically aligned CNTs in GelMA hydrogels yielded a higher number of functional myofibers than cells that were cultured on hydrogels with randomly distributed CNTs and horizontally aligned CNTs, as confirmed by the expression of myogenic genes and proteins. In addition, the myogenic gene and protein expression increased more profoundly after applying electrical stimulation along the direction of the aligned CNTs due to the anisotropic conductivity of the hybrid GelMA-vertically aligned CNT hydrogels. We believe that platform could attract great attention in other biomedical applications, such as biosensing, bioelectronics, and creating functional biomedical devices.

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“…A non-invasive glucose biosensor was later developed by Obregon et al . based on the cultured muscle tissue using a thin film of nanoporous gold [49]. By measuring the glucose uptake of the engineered skeletal muscle tissue with and without electrical stimulations, it was revealed that electrically stimulated muscle tissues consumed at least 10% more glucose when compared to normal tissues without electrical stimulation [49].…”

Section: Microfabrication Of Gelma Hydrogelsmentioning

confidence: 99%

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

et al. 2015

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Gelatin methacryloyl (GelMA) hydrogels have been widely used for various biomedical applications due to their suitable biological properties and tunable physical characteristics. Three dimensional (3D) GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM) due to the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs, which allow cells to proliferate and spread in GelMA-based scaffolds. GelMA is also versatile from a processing perspective. It crosslinks when exposed to light irradiation to form hydrogels with tunable mechanical properties which mimic the native ECM. It can also be microfabricated using different methodologies including micromolding, photomasking, bioprinting, self-assembly, and microfluidic techniques to generate constructs with controlled architectures. Hybrid hydrogel systems can also be formed by mixing GelMA with nanoparticles such as carbon nanotubes and graphene oxide, and other polymers to form networks with desired combined properties and characteristics for specific biological applications. Recent research has demonstrated the proficiency of GelMA-based hydrogels in a wide range of applications including engineering of bone, cartilage, cardiac, and vascular tissues, among others. Other applications of GelMA hydrogels, besides tissue engineering, include fundamental single-single cell research, cell signaling, drug and gene delivery, and bio-sensing.

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Non-invasive measurement of glucose uptake of skeletal muscle tissue models using a glucose nanobiosensor

Cited by 20 publications

References 37 publications

Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion

Electro-Acupuncture at Neiguan Pretreatment Alters Genome-Wide Gene Expressions and Protects Rat Myocardium against Ischemia-Reperfusion

Hybrid hydrogels containing vertically aligned carbon nanotubes with anisotropic electrical conductivity for muscle myofiber fabrication

Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels

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