Non-Invasive Measurement of Exercise-Induced Oxidative Stress in Response to Physical Activity. A Systematic Review and Meta-Analysis

Abstract

Physical activity may benefit health by modulating oxidative stress and inflammation. However, the selection of suitable exercise-induced oxidative stress biomarkers is still challenging. This study aimed at systematically summarizing the available evidence on exercise-induced oxidative stress measured in urine and/or saliva. Two meta-analyses including the most frequently quantified biomarkers of oxidative stress, namely, urinary isoprostane and DNA oxidation products, were performed. Three electronic databases (PubMed, EMBASE and Cochrane CENTRAL) were interrogated. Among 4479 records, 43 original articles were included in the systematic review and 11 articles were included in meta-analysis I and II, respectively. We observed a pooled trend of increase of urinary isoprostanes in response to physical activity (+0.95, 95% CI: −0.18; 2.09). In comparison with aerobic exercise, anaerobic training determined a greater induction of isoprostanes (+5.21, 95% CI: 2.76; 7.66, p < 0.0001), which were markedly increased after vigorous physical activity (+6.01, 95% CI: 1.18; 10.84, p < 0.001) and slightly decreased in response to exercise interventions protracted over time (e.g., months) (−1.19, 95% CI: −2.25; −0.12, p < 0.001). We recommend the most integrative approach of oxidative stress multi-marker panels in response to physical activity instead of selecting one preferential biomarker to quantify physical activity-induced oxidative stress in humans.

The Double-edged Sword of Antioxidant Supplements on Metabolic Diseases, A Necessity for Quantification of Oxidative Status

“…A simplified and commonly accessible analytical method has been developed and validated for the quantitative determination of blood 3NT to assess oxidative stress clinically [32]. Selection of an integrative panel of multi-markers is also recommended for the quantification of oxidative stress in humans [33) and 3NT should be one of them.…”

Effect of exercise and antioxidant supplementation on cellular lipid peroxidation in elderly individuals: Systematic review and network meta-analysis

“…In recent decades, the impact of physical exercise on oxidative stress has attracted extensive attention, although the understanding of exercise and oxidative stress has advanced, questions remain about whether exercise-induced increases in ROS production are beneficial to health or not ( Powers et al, 2020 ). Several previous studies have given some methodological advice, as well as several pieces of evidence with high-quality, suggesting that whether the effect of exercise-induced oxidative stress is positive or negative depends on the health status of individuals and calling for an integrative approach of multi-markers to measure the oxidative stress in response to physical exercise instead of selecting only one biomarker to quantify physical exercise-induced oxidative stress in humans ( Lu et al, 2021 ; Squillacioti et al, 2021 ).…”