Non‐invasive management of non‐melanoma skin cancer in patients with cancer predisposition genodermatosis: a role for confocal microscopy and photodynamic therapy

Segura, Sònia; Puig, Susana; Carrera, Cristina; Lecha, M; Borges, VF; Malvehy, Josep

doi:10.1111/j.1468-3083.2010.03871.x

Cited by 46 publications

(37 citation statements)

References 38 publications

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“…In contrast to Evangelou et al [23], who observed a significant decrease in bright dendritic-shaped Langerhans cells already 1 h after PDT, we and others [20] observed a significant increase in Langerhans cells 7 days after treatment. Given that Langerhans cells are important effector cells of the immune system, it has been speculated that their decreasing number or absence may indicate a reduced antitumoral T-cell response, which in turn may also influence the treatment response.…”

Section: Discussioncontrasting

confidence: 51%

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Confocal Microscopy Insights into the Treatment and Cellular Immune Response of Basal Cell Carcinoma to Photodynamic Therapy

et al. 2012

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Background: Photodynamic therapy (PDT) represents an optimal treatment for basal cell carcinoma (BCC). Reflectance confocal microscopy (RCM) is a noninvasive imaging tool that has been applied in skin oncology and for BCC diagnosis. Moreover, RCM is a useful tool to determine noninvasive treatment efficacy of nonmelanoma skin cancer. Objective: We aimed to investigate the role of RCM in assessing the efficacy of PDT in the treatment of BCC and to evaluate the skin changes following the PDT. Methods: Ten patients with 12 BCCs were treated with PDT. Dermoscopy and RCM imaging were performed at baseline as well as 7 days (T1), 30 days and 18 months after PDT. Cytological examination was taken at baseline and in case of BCC persistence. Results: At T1, RCM showed the presence of several dendritic-shaped cells within the epidermis, corresponding to activated Langerhans cells. After 1 month, RCM showed the persistence of 2 BCCs, which escaped the clinical and dermoscopic diagnosis. At the long-term follow-up, none of the tumors revealed signs of persistence or recurrence. Conclusion: RCM is a valuable noninvasive tool for the diagnosis and treatment monitoring of BCC using PDT.

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Section: Discussioncontrasting

confidence: 51%

“…The possibility to assess PDT efficacy with noninvasive techniques has recently been explored. RCM has been applied to monitor PDT efficacy in Gorlin Goltz patients [20] and for other BCC treatment options [21,22], demonstrating as an optimal tool in the hands of clinicians, to detect BCC persistence.…”

Section: Discussionmentioning

confidence: 99%

Confocal Microscopy Insights into the Treatment and Cellular Immune Response of Basal Cell Carcinoma to Photodynamic Therapy

et al. 2012

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“…Its depth sectioning capability and reflection or fluorescence sensitivity can be utilized for cancer diagnosis in thin (~200-300 µm) samples [14][15][16]. CM systems offering cellular resolution have been successfully employed in several clinical investigations to identify nonmelanoma skin lesions and to monitor treatment responses in vivo [17]. However, the potential cellular and subcellular level resolution in lateral direction is accompanied by penetration and axial resolution limitations for optical imaging techniques (Figure 1).…”

Section: Imaging In Dermatologymentioning

confidence: 98%

3D optical coherence tomography for clinical diagnosis of nonmelanoma skin cancers

Alex¹,

Eibl²,

Binder³

et al. 2011

Imaging in Medicine

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High-resolution frequency domain optical coherence tomography (OCT) stands out amongst a range of novel dermatologic imaging technologies, with its good detection sensitivity around -100 dB, high measurement speeds allowing real-time image acquisition and its ability to acquire high definition crosssectional and 3D tomograms of regions greater than 1 cm 2 , providing tissue information comparable to conventional histopathology without the need for any contrast agents. Typical axial and transverse resolutions of state-of-the-art OCT systems range between 1-10 µm and approximately 20 µm, respectively, depending on the employed wavelength region. This review investigates the significant progress accomplished in the field of dermatologic OCT with respect to other in vivo diagnostic methods for preexcisional imaging of nonmelanoma skin cancers and specifically emphasizes state-of-the-art results achieved in different clinical pilot studies. Further technological extensions of OCT, various multimodal imaging approaches as well as potential clinical dermatologic applications are discussed.KEYWORDS: cancer diagnosis n contrast-enhanced imaging n dermatology n functional imaging n microscopy n multimodal imaging n optical coherence tomography n optical imaging n tomography

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“…This technique has been demonstrated to be effective in non-melanoma skin cancers (NMSCs) (1–5) as well as other non-oncogenic skin conditions such as acne (6–8) and warts (9–11). Silicon phthalocyanine (Pc) 4 is a second-generation PDT photosensitizer discovered and developed at Case Western Reserve University (5) that has been recently shown to be safe in Pc 4-PDT-treated patients with skin neoplasms and to produce clinical improvement in cutaneous T cell lymphoma lesions (12–14). Pc 4 offers several advantages for PDT: 1.)…”

Section: Introductionmentioning

confidence: 99%

Activated T cells exhibit increased uptake of silicon phthalocyanine Pc 4 and increased susceptibility to Pc 4-photodynamic therapy-mediated cell death

Soler

Ohtola

Sugiyama

et al. 2016

Photochem Photobiol Sci

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Photodynamic therapy (PDT) is an emerging treatment for malignant and inflammatory dermal disorders. Photoirradiation of the silicon phthalocyanine (Pc) 4 photosensitizer with red light generates singlet oxygen and other reactive oxygen species to induce cell death. We previously reported that Pc 4-PDT elicited cell death in lymphoid-derived (Jurkat) and epithelial-derived (A431) cell lines in vitro, and furthermore that Jurkat cells were more sensitive than A431 cells to treatment. In this study, we examined the effectiveness of Pc 4-PDT on primary human CD3+ T cells in vitro. Fluorometric analyses of lysed T cells confirmed the dose-dependent uptake of Pc 4 in non-stimulated and stimulated T cells. Flow cytometric analyses measuring annexin V and propidium iodide (PI) demonstrated a dose-dependent increase of T cell apoptosis (6.6–59.9%) at Pc 4 doses ranging from 0–300 nM. Following T cell stimulation through the T cell receptor using a combination of anti-CD3 and anti-CD28 antibodies, activated T cells exhibited increased susceptibility to Pc 4-PDT-induced apoptosis (10.6–81.2%) as determined by Pc 4 fluorescence in each cell, in both non-stimulated and stimulated T cells, Pc 4 uptake increased with Pc 4 dose up to 300 nM as assessed by flow cytometry. The mean fluorescence intensity (MFI) of Pc 4 uptake measured in stimulated T cells was significantly increased over the uptake of resting T cells at each dose of Pc 4 tested (50, 100, 150 and 300nM, p<0.001 between 50 and 150nM, n=8). Treg uptake was diminished relative to other T cells. Cutaneous T cell lymphoma (CTCL) T cells appeared to take up somewhat more Pc 4 than normal resting T cells at 100 and 150nm Pc 4. Confocal imaging revealed that Pc 4 localized in cytoplasmic organelles, with approximately half of the Pc 4 co-localized with mitochondria in T cells. Thus, Pc 4-PDT exerts an enhanced apoptotic effect on activated CD3+ T cells that may be exploited in targeting T cell-mediated skin diseases, such as cutaneous T cell lymphoma (CTCL) or psoriasis.

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Non‐invasive management of non‐melanoma skin cancer in patients with cancer predisposition genodermatosis: a role for confocal microscopy and photodynamic therapy

Cited by 46 publications

References 38 publications

Confocal Microscopy Insights into the Treatment and Cellular Immune Response of Basal Cell Carcinoma to Photodynamic Therapy

Confocal Microscopy Insights into the Treatment and Cellular Immune Response of Basal Cell Carcinoma to Photodynamic Therapy

3D optical coherence tomography for clinical diagnosis of nonmelanoma skin cancers

Activated T cells exhibit increased uptake of silicon phthalocyanine Pc 4 and increased susceptibility to Pc 4-photodynamic therapy-mediated cell death

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