Non‐invasive lung cancer diagnosis by detection of<i><scp>GATA</scp>6</i>and<i><scp>NKX</scp>2‐1</i>isoforms in exhaled breath condensate

Mehta, Aditi; Cordero, Julio; Dobersch, Stephanie; Romero‐Olmedo, Addi J.; Savai, Rajkumar; Bodner, Johannes; Chao, Cho-Ming; Fink, Ludger; Guzman-Diaz, Ernesto; Singh, Indrabahadur; Dobreva, Gergana; Rapp, Ulf R.; Günther, Stefan; Ilinskaya, О. N.; Bellusci, Savério; Dammann, Reinhard; Braun, Thomas; Seeger, Werner; Gattenlöhner, Stefan; Tresch, Achim; Günther, Andreas; Barreto, Guillermo

doi:10.15252/emmm.201606382

Cited by 28 publications

(18 citation statements)

References 41 publications

(47 reference statements)

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“…A recent study suggests that modulating the expression of GATA6 may affect the growth of NSCLC cells ( 27 ). Mehta et al verified GATA6 and NKX2-1 as diagnostic biomarkers of non-invasive lung cancer ( 28 ). BMP4 regulation by GATA6 showed that it can inhibit tumorigenesis and metastasis of lung adenocarcinoma cells ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

miR-196b promotes lung cancer cell migration and invasion through the targeting of GATA6

2018

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MicroRNAs (miRNAs) have been proven to regulate gene expression at the protein translation level. miRNA abnormal expression has been associated with the development of lung cancer. In the present study, we aimed to investigate the mechanism of miR-196 in non-small cell lung cancer (NSCLC). The miR-196b and GATA-6 (GATA6) expression levels were examined in NSCLC by RT-qPCR and western blot analysis. Transwell assay was used to assess cell migration and invasion. Moreover, the specific target of miR-196b in NSCLC was verified by the luciferase reporter assay. The expression of miR-196b was higher in both NSCLC tissues and cells than the normal levels. Specifically, the miR-196b mimic group in NSCLC cells markedly promoted cell migration and invasion, while the miR-196b inhibitor group exhibited the opposite effect. Furthermore, GATA6 was verified as a specific target of miR-196b in NSCLC cells and GATA6 could attenuate the migratory and invasive ability of NSCLC cells regulated by miR-196b. In addition, the relationship between GATA6 and miR-196b expression was negatively correlated in NSCLC tissues. Thus, miR-196b enhanced NSCLC cell migration and invasion via the downregulation of GATA6, indicating its potential application in NSCLC diagnosis and therapy.

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Section: Discussionmentioning

confidence: 99%

miR-196b promotes lung cancer cell migration and invasion through the targeting of GATA6

2018

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“…GATA6 is a member of the GATA transcription regulator family. As a gene that is closely related to the occurrence and development of diseases, it is involved in a variety of biological behaviors of cells and has a significant relationship with the process of different tumors [7][8][9]. Therefore, it is of great importance to fully understand and study the GATA6 gene.…”

Section: Discussionmentioning

confidence: 99%

The expression of the GATA6 gene in oral carcinoma cell lines

Guan

2021

World J Surg Onc

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Background This study aimed to investigate the expression level of the GATA6 gene in different oral cancer cells. Methods In this study, we sub-cultured normal oral epithelial cell lines HOK, human tongue squamous cell carcinoma cell lines CAL-27 and SCC-4, and human salivary gland adenoid cystic carcinoma cell lines SACC-LM and SACC-83. Subsequently, we used reverse transcription-polymerase chain reaction RT-PCR and Western blot methods to detect the mRNA and the protein expressions of GATA6 in normal oral epithelial cells, human tongue squamous cell carcinoma cells, and human salivary gland adenoid cystic carcinoma cells. Results The results of this study showed that the mRNA expression levels of GATA6 in CAL-27, SCC-4, and SACC-LM cells were significantly increased when compared with the HOK cells. However, the mRNA expression level of GATA6 in the SACC-83 cells had no significant difference compared with the HOK cells. The protein expression levels of GATA6 in the SCC-4 and SACC-LM cells were, however, significantly increased whereas the protein expression levels of GATA6 in the CAL-27 and SACC-83 cells had no significant difference when compared with the HOK cells. Conclusion The GATA6 gene may be related to the occurrence and progression of certain oral cancers.

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“…These genes are key regulators of lung development and each one gives rise to two different isoforms: the embryonic isoform (Em) mainly expressed during early developmental stages and the adult (Ad) isoform expressed during the later stages of lung development. These authors proved that Em/Ad expression ratio of GATA6 and NKX2-1 increased in the EBC of lung cancer patients compared with EBC extracted from control subjects, highlighting the potential use of EBC for lung cancer detection [47].…”

Section: Ebc Analysismentioning

confidence: 91%

“…Another type of nucleic acid present in EBC is RNA. There have been no published data on transcriptomics studies on EBC, and very little is known of messenger RNAs present in this biofluid [28] because of the high fragmentation of the RNA isolated from EBC [47].…”

Section: Ebc Analysismentioning

confidence: 99%

Exhaled breath condensate biomarkers for lung cancer

2019

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Lung cancer is the main cause of cancer incidence and mortality worldwide and the identification of clinically useful biomarkers for lung cancer detection at both early and metastatic stage is a pressing medical need. Although many improvements have been made in the treatment and in the early screening of this cancer, most diagnosis are made at a late stage, when a lot of genetic and epigenetic changes have occurred. A promising source of biomarkers reflective of the pathogenesis of lung cancer is exhaled breath condensate (EBC), a biological fluid and a natural matrix of the respiratory tract. Molecules such as DNAs, RNAs, proteins, metabolites and volatile compounds are present in EBC, and their presence/absence or their variation in concentrations can be used as biomarkers. The aims of this review are to briefly describe exhaled breath composition, firstly, and then to document some of the EBC candidate biomarkers for lung cancer by dividing them according to their origin (genome, transcriptome, epigenome, metabolome, proteome and microbiota) in order to demonstrate the potential use of EBC as a helpful tool in cancer diagnostics, molecular profiling, therapy monitoring and screening of high risk individuals.

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Non‐invasive lung cancer diagnosis by detection ofGATA6andNKX2‐1isoforms in exhaled breath condensate

Cited by 28 publications

References 41 publications

miR-196b promotes lung cancer cell migration and invasion through the targeting of GATA6

miR-196b promotes lung cancer cell migration and invasion through the targeting of GATA6

The expression of the GATA6 gene in oral carcinoma cell lines

Exhaled breath condensate biomarkers for lung cancer

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