Non-invasive Investigation of Tumor Metabolism and Acidosis by MRI-CEST Imaging

Consolino, Lorena; Anemone, Annasofia; Capozza, Martina; Carella, Antonella; Irrera, Pietro; Corrado, Alessia; Dhakan, Chetan; Bracesco, Martina; Longo, Dario Livio

doi:10.3389/fonc.2020.00161

Cited by 42 publications

(43 citation statements)

References 129 publications

(130 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indirect measurements of acidic regions in the TME have been obtained in canine OS samples by immunohistochemistry (IHC) analysis (Avnet et al , 2017). Consequently, non‐invasive imaging approaches are needed to provide accurate in vivo measurements of tumor acidosis (Anemone et al , 2019; Consolino et al , 2020). Previous MRS approaches reported intratumoral acidosis in murine fibrosarcoma models, but lacked the ability to assess the spatial distribution (Vaupel et al , 1989, 1994).…”

Section: Epidemiology Of Sarcomamentioning

confidence: 99%

Sarcoma treatment in the era of molecular medicine

et al. 2020

View full text Add to dashboard Cite

Sarcomas are heterogeneous and clinically challenging soft tissue and bone cancers. Although constituting only 1% of all human malignancies, sarcomas represent the second most common type of solid tumors in children and adolescents and comprise an important group of secondary malignancies. More than 100 histological subtypes have been characterized to date, and many more are being discovered due to molecular profiling. Owing to their mostly aggressive biological behavior, relative rarity, and occurrence at virtually every anatomical site, many sarcoma subtypes are in particular difficult‐to‐treat categories. Current multimodal treatment concepts combine surgery, polychemotherapy (with/without local hyperthermia), irradiation, immunotherapy, and/or targeted therapeutics. Recent scientific advancements have enabled a more precise molecular characterization of sarcoma subtypes and revealed novel therapeutic targets and prognostic/predictive biomarkers. This review aims at providing a comprehensive overview of the latest advances in the molecular biology of sarcomas and their effects on clinical oncology; it is meant for a broad readership ranging from novices to experts in the field of sarcoma.

show abstract

Section: Epidemiology Of Sarcomamentioning

confidence: 99%

Sarcoma treatment in the era of molecular medicine

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Another interesting application of CEST is pH imaging in which the endogenous amide proton transfer rate is related to the pH in the tissue of interest (62) . Most applications of CEST pH imaging are done in the brain (63)(64)(65) where the pH is between 6⋅5 and 8⋅5. It would be of great utility if CEST could be used to make 3-D stomach pH maps since pH is an important factor that among others influences pepsin activity.…”

Section: Chemical Exchange Saturation Transfermentioning

confidence: 99%

Monitoring food digestion with magnetic resonance techniques

et al. 2020

View full text Add to dashboard Cite

This review outlines the current use of magnetic resonance (MR) techniques to study digestion and highlights their potential for providing markers of digestive processes such as texture changes and nutrient breakdown. In vivo digestion research can be challenging due to practical constraints and biological complexity. Therefore, digestion is primarily studied using in vitro models. These would benefit from further in vivo validation. NMR is widely used to characterise food systems. MRI is a related technique that can be used to study both in vitro model systems and in vivo gastro-intestinal processes. MRI allows visualisation and quantification of gastric processes such as gastric emptying and coagulation. Both MRI and NMR scan sequences can be configured to be sensitive to different aspects of gastric or intestinal contents. For example, magnetisation transfer and chemical exchange saturation transfer can detect proton (1H) exchange between water and proteins. MRI techniques have the potential to provide molecular-level and quantitative information on in vivo gastric (protein) digestion. This requires careful validation in order to understand what these MR markers of digestion mean in a specific digestion context. Combined with other measures they can be used to validate and inform in vitro digestion models. This may bridge the gap between in vitro and in vivo digestion research and can aid the optimisation of food properties for different applications in health and disease.

show abstract

“…Moreover, the large heterogeneity that we observed in tumour pHe values among the investigated tumour murine models is in agreement with the range of pHe values reported by others using MRI-based approaches and confirmed by invasive microelectrode measurements. [60][61][62][63] Importantly, the relationship between tumour acidosis and metastatic propensity is a valuable clinical issue, since death rate in breast cancer patients is heavily dependent on metastases. In addition to the strong correlation observed between the acidity score and the number of lung metastases, we further investigated whether a causative link exists between tumour acidosis and invasiveness.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tumour acidosis evaluated in vivo by MRI-CEST pH imaging reveals breast cancer metastatic potential

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Tumour acidosis is considered to play a central role in promoting cancer invasion and migration, but few studies have investigated in vivo how tumour pH correlates with cancer invasion. This study aims to determine in vivo whether tumour acidity is associated with cancer metastatic potential. Methods Breast cancer cell lines with different metastatic potentials have been characterised for several markers of aggressiveness and invasiveness. Murine tumour models have been developed and assessed for lung metastases and tumour acidosis has been assessed in vivo by a magnetic resonance imaging-based chemical exchange saturation transfer (CEST) pH imaging approach. Results The higher metastatic potential of 4T1 and TS/A primary tumours, in comparison to the less aggressive TUBO and BALB-neuT ones, was confirmed by the highest expression of cancer cell stem markers (CD44+CD24−), highlighting their propensity to migrate and invade, coinciding with the measurement obtained by in vitro assays. MRI-CEST pH imaging successfully discriminated the more aggressive 4T1 and TS/A tumours that displayed a more acidic pH. Moreover, the observed higher tumour acidity was significantly correlated with an increased number of lung metastases. Conclusions The findings of this study indicate that the extracellular acidification is associated with the metastatic potential.

show abstract

Non-invasive Investigation of Tumor Metabolism and Acidosis by MRI-CEST Imaging

Cited by 42 publications

References 129 publications

Sarcoma treatment in the era of molecular medicine

Sarcoma treatment in the era of molecular medicine

Monitoring food digestion with magnetic resonance techniques

Tumour acidosis evaluated in vivo by MRI-CEST pH imaging reveals breast cancer metastatic potential

Contact Info

Product

Resources

About