Non-invasive image acquisition and advanced processing in optical bioimaging

Farkas, Daniel L.; Du, Congwu; Fisher, Gregory W.; Lau, Carmen; Niu, Wenhua; Wachman, E. S.; Levenson, Richard M.

doi:10.1016/s0895-6111(98)00011-1

Cited by 104 publications

(58 citation statements)

References 34 publications

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“…A number of previous reports have described fluorescence tomographic imaging systems of various sensitivities and resolutions (22,23), whereas a few have focused on high spatial resolution imaging systems for their ultimate use in mouse models (10). Most of the described reports to date have dealt with system optimization (13,24) and/or characterization of imaging systems using phantoms (25).…”

Section: Introductionmentioning

confidence: 99%

Tomographic Fluorescence Mapping of Tumor Targets

et al. 2005

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Methods that allow robust imaging of specific molecular targets and biological processes in vivo should have widespread applications in biology and clinical medicine.Here we use a quantitative, three-dimensional fluorescencemediated tomographic technique (FMT) that enables rapid measurements of fluorochrome-based affinity tags in live xenograft models. We validate the method by showing its sensitivity in quantitating tumor angiogenesis and therapeutic modulation using an anti-vascular endothelial growth factor antibody. Furthermore, we show the feasibility of simultaneous multichannel measurements of distinct biological phenomena such as receptor tyrosine kinase expression and angiogenesis. FMT measurements can be done serially, with short imaging times and within the same live animal. The described method should be valuable for rapidly profiling biological phenomena in vivo. (Cancer Res 2005; 65(14): 6330-6)

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Section: Introductionmentioning

confidence: 99%

Tomographic Fluorescence Mapping of Tumor Targets

et al. 2005

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“…Several modalities of spectral imaging systems adaptable to biomedical studies have been described in the literature. 15 A popular system is comprised of coupling a liquid crystal tunable optical filter (LCTF) with a regular light microscope. 16 The LCTF uses electrically controlled liquid crystal elements that transmit certain wavelengths while blocking others and it can operate in the visible range (420 -720 nanometers [nm]) at intervals as narrow as 1 nm.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic classification of urothelial cells in urine cytology specimens using exclusively spectral information

Jaganath

Angeletti

Levenson

et al. 2004

Cancer

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“…These algorithms utilize the spectral characteristics of the fluorophores and the autofluorescence. 6 However, the measurement procedure can be quite complex since one needs to acquire the emission at multiple wavelengths. To suppress the effects of autofluorescence, several approaches have been suggested.…”

Section: -3mentioning

confidence: 99%

Autofluorescence insensitive imaging using upconverting nanocrystals in scattering media

Svensson

Axelsson

et al. 2008

Applied Physics Letters

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Autofluorescence is a nuisance in the field of fluorescence imaging and tomography of exogenous molecular markers in tissue, degrading the quality of the collected data. In this letter, we report autofluorescence insensitive imaging using highly efficient upconverting nanocrystals (NaYF4:Yb3+∕Tm3+) in a tissue phantom illuminated with near-infrared radiation of 85mW∕cm2. It was found that imaging with such nanocrystals leads to an exceptionally high contrast compared to traditional downconverting fluorophores due to the absence of autofluorescence. Upconverting nanocrystals may be envisaged as important biological markers for tissue imaging purposes.

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Non-invasive image acquisition and advanced processing in optical bioimaging

Cited by 104 publications

References 34 publications

Tomographic Fluorescence Mapping of Tumor Targets

Tomographic Fluorescence Mapping of Tumor Targets

Diagnostic classification of urothelial cells in urine cytology specimens using exclusively spectral information

Autofluorescence insensitive imaging using upconverting nanocrystals in scattering media

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