“…We evaluated the longitudinal modulus of the colonoids via Brillouin microscopy using a home-built setup that was described previously. 90 Firstly, general morphology was assessed using a transmitted light microscope. For the Brillouin measurements, the colonoids stayed in colonoid growth medium and were imaged using a 10x/0.28 objective (M Plan Apo, Edmund Optics Inc., Barrington, NJ, US).…”