Non-invasive diffuse correlation tomography reveals spatial and temporal blood flow differences in murine bone grafting approaches

Han, Songfeng; Proctor, Ashley R.; Vella, Joseph; Benoit, Danielle S. W.; Choe, Regine

doi:10.1364/boe.7.003262

Cited by 19 publications

(24 citation statements)

References 68 publications

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“…This limitation resulted in the lack of blood flow information during the tumor growth prior to treatment. To address these limitations, a non-contact scanning diffuse correlation tomography system [72,73] will be adapted for animal cancer monitoring. This will enable measurements of tumors at any time during their development and provide reliable three-dimensional blood flow imaging with spatially dense datasets from the scanning system.…”

Section: Limitations and Future Studiesmentioning

confidence: 99%

Chemotherapeutic drug-specific alteration of microvascular blood flow in murine breast cancer as measured by diffuse correlation spectroscopy

Ramirez¹,

Proctor²,

Jung³

et al. 2016

Biomed. Opt. Express

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Abstract:The non-invasive, in vivo measurement of microvascular blood flow has the potential to enhance breast cancer therapy monitoring. Here, longitudinal blood flow of 4T1 murine breast cancer (N=125) under chemotherapy was quantified with diffuse correlation spectroscopy based on layer models. Six different treatment regimens involving doxorubicin, cyclophosphamide, and paclitaxel at clinically relevant doses were investigated. Treatments with cyclophosphamide increased blood flow as early as 3 days after administration, whereas paclitaxel induced a transient blood flow decrease at 1 day after administration. Early blood flow changes correlated strongly with the treatment outcome and distinguished treated from untreated mice individually for effective treatments. characterization and therapeutic relevance," Int.

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Section: Limitations and Future Studiesmentioning

confidence: 99%

Chemotherapeutic drug-specific alteration of microvascular blood flow in murine breast cancer as measured by diffuse correlation spectroscopy

Ramirez¹,

Proctor²,

Jung³

et al. 2016

Biomed. Opt. Express

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“…In our laboratory, a noncontact DCT technique has been developed for assessing blood flow in a murine femoral graft model. The DCT technique has been verified and applied in previous tissue phantom and longitudinal bone graft monitoring studies . In our weekly bone graft measurements, blood flow in grafts increased at one week after graft surgery.…”

Section: Introductionmentioning

confidence: 60%

“…Estimation of the blood flow distribution αD b ( r ) based on the measured g 2 on tissue surface is determined by minimizing the objective function

χ^{2} = \frac{1}{2} {false\sum}_{i = 1}^{N_{sd}} {(||, \ln ()(, \frac{g_{1, m} ({bold-italicr}_{italicsi}, {bold-italicr}_{italicdi}, τ)}{g_{1, c} ({bold-italicr}_{italicsi}, {bold-italicr}_{italicdi}, τ)}))}^{2}

. Here, g 1,c is the calculated autocorrelation function based on an estimate of blood flow distribution, g 1,m is the experimentally obtained autocorrelation function, N sd is the total number of the source‐detector pairs, and r si and r di are the locations of the source and detector.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal 3D Blood Flow Distribution Provided by Diffuse Correlation Tomography during Bone Healing in a Murine Fracture Model

Ren

Han

Proctor

et al. 2020

Photochem & Photobiology

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Noninvasive monitoring of vascularization can potentially diagnose impaired bone healing earlier than current radiographic methods. In this study, a noncontact diffuse correlation tomography (DCT) technique was employed to measure longitudinal blood flow changes during bone healing in a murine femoral fracture model. The three‐dimensional distribution of the relative blood flow was quantified from one day pre‐fracture to 48 days post‐fracture. For three mice, frequent DCT measurements were performed every other day for one week after fracture, and then weekly thereafter. A decrease in blood flow was observed in the bone fracture region at one day post‐fracture, followed by a monotonic increase in blood flow beyond the pre‐injury baseline until five to seven days post‐fracture. For the remaining 12 mice, only weekly DCT measurements were performed. Data collected on a weekly basis show the blood flow for most mice was elevated above baseline during the first two post‐fracture weeks, followed by a subsequent decrease. Torsional strength of the excised femurs was measured for all 15 mice after 7 weeks of healing. A metric based on the early blood flow changes shows a statistically significant difference between the high strength group and the low strength group.

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“…DCT has been studied in both preclinical and clinical settings to monitor blood flow changes in mouse and rat brains [ 14 , 15 ], and in human breast [ 16 ]. We have developed a non-invasive DCT system to monitor 3-dimensional blood flow changes in the mouse femur [ 17 , 18 ]. With the DCT system, we have revealed that blood flow changes are different among three groups of autografts, allografts, and T.E.…”

Section: Introductionmentioning

confidence: 99%

“…Weekly DCT measurements were performed on three groups of mice for 9 weeks, adhering to the same protocol reported previously [ 17 ]. To independently quantify graft healing, we utilized an established biomechanical testing method to measure maximum bone torque after 9-week healing, which has been widely used as an indicator for long-term healing [ 6 , 8 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Temporal blood flow changes measured by diffuse correlation tomography predict murine femoral graft healing

et al. 2018

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Blood flow changes during bone graft healing have the potential to provide important information about graft success, as the nutrients, oxygen, circulating cells and growth factors essential for integration are delivered by blood. However, longitudinal monitoring of blood flow changes during graft healing has been a challenge due to limitations in current techniques. To this end, non-invasive diffuse correlation tomography (DCT) was investigated to enable longitudinal monitoring of three-dimensional blood flow changes in deep tissue. Specific to this study, longitudinal blood flow changes were utilized to predict healing outcomes of common interventions for massive bone defects using a common mouse femoral defect model. Weekly blood flow changes were non-invasively measured using a diffuse correlation tomography system for 9 weeks in three types of grafts: autografts (N = 7), allografts (N = 6) and tissue-engineered allografts (N = 6). Healing outcomes were quantified using an established torsion testing method 9 weeks after transplantation. Analysis of the spatial and temporal blood flow reveals that major differences among the three groups were captured in weeks 1–5 after graft transplantation. A multivariate model to predict maximum torque by relative blood flow changes over 5 weeks after graft transplantation was built using partial least squares regression. The results reveal lower bone strength correlates with greater cumulative blood flow over an extended period of time (i.e., 1–5 weeks). The current research demonstrates that DCT-measured blood flow changes after graft transplantation can be utilized to predict long-term healing outcomes in a mouse femoral graft model.

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Non-invasive diffuse correlation tomography reveals spatial and temporal blood flow differences in murine bone grafting approaches

Cited by 19 publications

References 68 publications

Chemotherapeutic drug-specific alteration of microvascular blood flow in murine breast cancer as measured by diffuse correlation spectroscopy

Chemotherapeutic drug-specific alteration of microvascular blood flow in murine breast cancer as measured by diffuse correlation spectroscopy

Longitudinal 3D Blood Flow Distribution Provided by Diffuse Correlation Tomography during Bone Healing in a Murine Fracture Model

Temporal blood flow changes measured by diffuse correlation tomography predict murine femoral graft healing

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